Method for simultaneously extracting and separating chlorogenic acid, solanesol, alkaloid and rutin in tobacco

Abstract

The invention relates to a method for simultaneously extracting and separating chlorogenic acid, solanesol, alkaloid and rutin in tobacco. The method comprises the following steps: 1, extracting simultaneously, namely, crushing a tobacco raw material, extracting by using 80% ethanol once under the conditions that the temperature is 40-50 DEG C, the ultrasonic frequency is 45KHz and the ratio of material to liquid is 10:1, and performing suction filtration or centrifugation, respectively extracting the residue by using 80% and 95% ethanol once; combining the extract solutions of three times, thereby obtaining crude extract of chlorogenic acid, solanesol, alkaloid and rutin in tobacco, wherein the extraction rates of chlorogenic acid, solanesol, alkaloid and rutin in tobacco are 100%; and 2, separating, namely, separating solanesol, separating chlorogenic acid and rutin, and separating alkaloid. By adopting the method, simultaneous and efficient extraction on chlorogenic acid, solanesol, alkaloid and rutin in tobacco is achieved, the extraction rates are all 100%, the four components are effectively separated through simple steps, the utilization rate of the tobacco resource is improved, at the same time the operation is simple, the organic solvent is saved, and the energy consumption is reduced.

Description

technical field [0001] The invention relates to a comprehensive extraction and separation technology of tobacco functional components, in particular to a method for simultaneous extraction and separation of tobacco chlorogenic acid, solanesol, nicotine and rutin. Background technique [0002] The medicinal value of tobacco has been discussed in Chinese herbal medicine and traditional Chinese medicine literature. Modern analytical chemistry has proved that tobacco is rich in a variety of biologically active functional components. Tobacco is the most abundant plant solanesol (1-4%) resource; the content of chlorogenic acid (0.2-5.4%) in tobacco leaves is equivalent to that of honeysuckle; The functional components are comparable; nicotine and its related substances in tobacco are very promising as future drug candidates for the treatment of Alzheimer's disease. Nicotine pesticide is a traditional "green pesticide" with high efficiency and low toxicity. Tobacco also contains ...

AI Technical Summary

Problems solved by technology

However, at present, the research and utilization of tobacco functional components are mainly based on the extraction and utilization technology of a single functional component. Although the comprehensive extraction technology of different functional components has begun to receive attention, it is mainly the comprehensive extraction technology of solanesol and nicotine. There are few reports on the comprehensive extraction technology of substances. Ai Xinxin of Jiangnan University reported the technology of simultaneously extracting tobacco solanesol, chlorogenic acid, and alkaloids with 95% ethanol ultrasonic assistance, and the extraction rates were 82% and 94% respectively. , 95%, the extraction efficiency of the three substances, especially solanesol, is low, and the extraction temperature required for the test is as high as 80°C, and the energy consumption of the test is high

[0004] It can be seen that most of the current technologies are single or a few extractions, and the extraction process is not ideal or the extraction rate is not high. At present, the comprehensive extraction of chlorogenic acid, solanesol, nicotine, and rutin from tobacco is efficiently extracted at the same time, and the realization of Solanesol, chlorogenic acid, rutin, and nicotine are effectively separated and the operation is simple, solvent-saving, and energy-saving technologies have not been reported yet

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