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Yeast strain for lactic acid production by using pentose and hexose

A technology for Saccharomyces cerevisiae, lactic acid, applied in bulk chemical production, microorganism-based methods, enzymes, etc., can solve problems such as limitations

Inactive Publication Date: 2014-10-01
FAR EASTERN NEW CENTURY COPRRATION
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

To sum up, although the production rate or production process of the lactic acid production technology developed by the conventional technology using strain improvement has been improved, the available carbon source is still limited when it is used in mass production, so a kind of lactic acid production technology has been developed. Strains that can produce higher lactate production, as well as low-cost production methods that can produce higher lactate production are still in demand

Method used

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  • Yeast strain for lactic acid production by using pentose and hexose
  • Yeast strain for lactic acid production by using pentose and hexose
  • Yeast strain for lactic acid production by using pentose and hexose

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example

[0046] Cloning of Xylose Metabolism Related Genes and Construction of Recombinant Plastids

[0047] In this example, the selection and colonization of genes related to xylose metabolism is carried out by using polymerase chain reaction (Polymerase chain reaction, PCR). First, use the primer combination of SEQ ID NO.1 / SEQ ID NO.2, SEQ ID NO.3 / SEQ ID NO.4, and use the gene library extracted from Saccharomyces cerevisiae BCRC22743 as a template to select and clone the pGK promoter and pGK terminator; with the primer combination of SEQ ID NO.5 / SEQ ID NO.6, SEQ ID NO.7 / SEQ ID NO.8, xylose reductase (XR), wood Sugar alcohol dehydrogenase (XDH) gene; with the primer combination of SEQ ID NO.9 / SEQ ID NO.10, and using the gene library extracted from Saccharomyces cerevisiae BCRC22743 as a template, xylulokinase (XKS) was selected by PCR Gene, construct the above nucleic acid fragments into the pAUR101 pAUR101 plastid with appropriate restriction enzymes, that is, complete the construc...

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Abstract

The present invention provides a biologically pure culture of Saccharomyces cerevisiae, which culture has a characteristic nature capable of highly producing lactic acid. A method for preparing the biologically pure culture and a method for production of lactic acid are also provided.

Description

technical field [0001] The present invention relates to the production technology of lactic acid, in detail, relates to Saccharomyces cerevisiae and its method for high-volume production of lactic acid. Background technique [0002] Traditional lactic acid production is obtained by fermentation of lactic acid bacteria (Lactobacillus spp.), but the cultivation of lactic acid bacteria requires the use of a compound medium. The cost of the compound medium is high. In addition to increasing the cost of the fermentation process, it is also increased from the fermentation broth The cost of separating and purifying lactic acid. However, if polylactic acid polymerized with lactic acid as a monomer is to be used in daily-use plastics, the cost of producing lactic acid must be reduced. Therefore, many studies have begun to try to improve strains, such as the use of Saccharomyces cerevisiae for microbial Fermented to produce lactic acid. The original strain of Saccharomyces cerevisia...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/19C12P7/56C12R1/865
CPCC12N15/81C12P7/56C12N9/0006C12P2203/00C12Y101/01027C12N1/185C12R2001/865Y02P20/52
Inventor 吕立婷黄德仁
Owner FAR EASTERN NEW CENTURY COPRRATION
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