Cell separating micro-structural system

Abstract

The invention relates to the technical field of biological and medical in vitro detection, and discloses a cell separating micro-structural system. The cell separating micro-structural system comprises a microfluidic chip, wherein the microfluidic chip comprises a sample inlet, a sample outlet, a cell capturing region, a side flushing inlet, a recycling outlet and a cleaning inlet; a plurality of microcolumns are formed inside the cell capturing region to form a microcolumn array; the microcolumn array is distributed in a gradient manner, thus showing microcolumn distance gradient change and microcolumn dimension gradient change; each layer of the microcolumn array is distributed in the form of a regular trapezoid gradually dispersing from the top layer to the bottom layer; the horizontal cross section shape of each microcolumn is divided into an upper part and a lower part; the upper part close to the sample inlet is similar to a normal isosceles triangle, wherein the two isosceles sides are in an symmetrical inner-concave circular arc shape; and the lower part close to the sample outlet is in an inverted isosceles triangle shape. The cell separating micro-structural system disclosed by the invention is used for separating, gathering, counting and recycling CTC or UETC, wherein detecting efficiency exceeds 90%; and moreover, damages of the microcolumn array on captured cells when a fluid impacts the captured cells are lowered.

Description

technical field [0001] The invention relates to the technical field of biological and medical in vitro detection, in particular to a microstructure system that can be used to separate and enrich rare cells from biological fluids, and the system can be used for circulating tumor cells (Circulating Tumor Cell, CTC) and Separation, enrichment, enumeration and recovery of Urine Exfoliated Tumor Cell (UETC) in urine. Background technique [0002] Rare cells refer to the rare cells that exist in biological samples and do have important biological functions or clinical detection significance. For example, circulating tumor cells, circulating endothelial cells, and virus-infected cells in human peripheral blood, tumor stem cells in blood vessels of solid tumors, exfoliated tumor cells in human lymph, exfoliated tumor cells in human urine, etc. [0003] 1. CTC isolation and enrichment and subsequent detection technology. [0004] Circulating tumor cells in human blood are a represe...

AI Technical Summary

Problems solved by technology

Urinary exfoliation cytology detection is currently considered the "gold standard" for non-invasive detection of bladder cancer. Although it has high specificity (nearly 100%) and high sensitivity for high-grade bladder cancer, it is not effective for low-grade bladder cancer. The sensitivity of diagnosis of early or early bladder cancer is low (only 30-50%), and the accuracy of urine cytology detection also depends on the experience of pathologists, which is more subjective, and the perfusion of chemical drugs in the bladder is also difficult. May cause false positives

[0018] At present, the UETC-based separation, enrichment and subsequent detection technology has not yet come out

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