A fermented bacterium agent for promoting ripening and increasing flavor of bean chili sauce and its preparation method
A technology of bean chili sauce and fermented bacteria agent, which is applied in the direction of biochemical equipment and methods, methods based on microorganisms, bacteria, etc., can solve problems such as difficult product quality assurance, difficult control of hygienic indicators, unstable koji quality, etc., and achieve improvement Product quality, shortened production cycle, and easy operation
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Embodiment 1
[0022] A kind of preparation method of fermented bacterium agent for promoting ripening and increasing flavor of watercress chili sauce, comprises the steps:
[0023] (1) Propagation and cultivation:
[0024] Bacillus pumilus (Bacillus pumilus) ACCC10615 (from the China Agricultural Microorganism Culture Collection and Management Center) was inoculated in the nutrient broth medium at 37°C for 24 hours, and the colony count reached 10 6 -10 9 cfu / mL, centrifuge to take the precipitate, and distilled water to elute the precipitate 2-4 times to prepare resting cells;
[0025] Lactobacillus fermentum (L. fermentum) CCTCCM87079 (from China Center for Type Culture Collection) and Lactobacillus bifermentum (L. bifermentans) CGMCC1.1876 (from JCM Japan Culture Collection of Microorganisms), After passing the activation and pure inspection respectively, they were inoculated in MRS liquid medium at 42°C, cultivated for 36 hours, and the colony count reached 10 6 -10 9 cfu / mL, centri...
Embodiment 2
[0035] A kind of preparation method of fermented bacterium agent for promoting ripening and increasing flavor of watercress chili sauce, comprises the steps:
[0036] (1) Propagation and cultivation:
[0037] Bacillus pumilus (Bacillus pumilus) ACCC10615 (sourced from China Agricultural Microorganism Culture Collection and Management Center) was inoculated in nutrient broth medium at 35°C for 24 hours, and the colony count reached 10 6 -10 9 cfu / mL, centrifuge to take the precipitate, and distilled water to elute the precipitate 2-4 times to prepare resting cells;
[0038]Lactobacillus fermentum (L. fermentum) CCTCCM87079 (from China Center for Type Culture Collection) and Lactobacillus bifermentum (L. bifermentans) CGMCC1.1876 (from JCM Japan Culture Collection of Microorganisms), After passing the activation and pure test respectively, they were inoculated in MRS liquid medium at 40°C, cultured for 36 hours, and the colony count reached 10 6 -10 9 cfu / mL, centrifuge to t...
Embodiment 3
[0048] A kind of preparation method of fermented bacterium agent for promoting ripening and increasing flavor of watercress chili sauce, comprises the steps:
[0049] (1) Propagation and cultivation:
[0050] Bacillus pumilus (Bacillus pumilus) ACCC10615 (sourced from China Agricultural Microorganism Culture Collection and Management Center) was inoculated in nutrient broth medium at 36°C for 24 hours, and the colony count reached 10 6 -10 9 cfu / mL, centrifuge to take the precipitate, and distilled water to elute the precipitate 2-4 times to prepare resting cells;
[0051] Lactobacillus fermentum (L. fermentum) CCTCCM87079 (from China Center for Type Culture Collection) and Lactobacillus bifermentum (L. bifermentans) CGMCC1.1876 (from JCM Japan Culture Collection of Microorganisms), After passing the activation and pure test respectively, inoculate in MRS liquid medium at 41°C, cultivate for 36 hours, and the colony count reaches 10 6 -10 9 cfu / mL, centrifuge to take the p...
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