Method for screening monoclonal antibody hybridoma by utilization of visual antigen chip

A hybridoma, monoclonal antibody technology, applied in the direction of material analysis by observing the effect on chemical indicators, analysis by chemical reaction of the material, measuring device, etc., can solve the problem of monoclonal antibody hybridoma cell screening technology The problems of long cycle and high cost can achieve the effect of shortening the preparation cycle, high affinity and good stability

Inactive Publication Date: 2014-09-24
UNIV OF SHANGHAI FOR SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] Aiming at the defects existing in the above-mentioned existing monoclonal antibody hybridoma cell screening technology, the technical problem to be solved by the present invention is to provide a method for scre...

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  • Method for screening monoclonal antibody hybridoma by utilization of visual antigen chip
  • Method for screening monoclonal antibody hybridoma by utilization of visual antigen chip

Examples

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Embodiment 1

[0023] Example 1 Screening of shigella monoclonal antibody hybridoma cells

[0024] The 17 Shigella monoclonal antibody hybridoma cells were screened by using a method for screening monoclonal antibody hybridoma cells provided by the present invention using a visualized antigen chip. The specific method is as follows:

[0025] (1) Preparation of antigen chip

[0026] combine figure 1 Shown, 39 kinds of Shigella and other pathogenic bacteria (antigen analogs) in Table 1 (concentration is 10 8 CFU / mL), as well as the positive control and negative control were spotted on the nitrocellulose membrane with a biochip spotter, and then stored at 4°C for later use.

[0027] The size of the substrate is 16mm×16mm, and the fixed sample point array is as follows: figure 1 As shown, the first row is the pathogenic bacteria No. 1-7; the second row is the pathogenic bacteria No. 8-14; the third row is the pathogenic bacteria No. 15-21; the fourth row is the pathogenic bacteria No. 22-28 ...

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Abstract

The invention discloses a method for screening monoclonal antibody hybridoma by the utilization of a visual antigen chip. Target antigens or target antigen analogues needed for screening the monoclonal antibody hybridoma are fixed to a substrate in a sample application mode, the prepared antigen chip is soaked into confining liquid for confining, then the antigen chip is soaked into cell supernatant which is generated by the positive hybridoma obtained at the early stage of monoclonal antibody preparation, then the antigen chip is sequentially soaked into enzyme-labeled secondary antibody and a developing solution for developing, and if brown or black blue spots develop on sampling points, it is judged that specific immune response exists between the hybridoma and the target antigens or the target antigen analogues fixed to the sampling points; if no spots develop on the sampling points, it is judged that no specific immune response exists between the hybridoma and the target antigens or the target antigen analogues. The screening period of the monoclonal antibody hybridoma is shortened, and production cost is reduced.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for screening monoclonal antibody hybridoma cells, in particular to a method for screening monoclonal antibody hybridoma cells by using a visualized antigen chip. Background technique [0002] Biochip technology is a high-throughput, large-scale parallel analysis and detection technology developed in the early 1990s. It has been rated as one of the top ten scientific and technological breakthroughs of the year by Science magazine twice. It utilizes specific interactions between molecules The principle is an analysis and detection technology that integrates multiple technologies (such as biochemistry, microprocessing, microelectronics, computers, etc.). Different from traditional analysis and detection methods, the microarray formed by it makes the biochemical analysis reaction process highly integrated on a certain carrier, and high-throughput analysis and detect...

Claims

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Application Information

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IPC IPC(8): G01N33/577G01N21/78
CPCG01N33/543G01N33/577
Inventor 刘箐邱实
Owner UNIV OF SHANGHAI FOR SCI & TECH
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