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Leech pepsin enzymolytic product and application thereof

A technology of pepsin and enzymatic hydrolyzate, applied in the field of leech pepsin enzymolyzate and its application, can solve the problems of microbial pollution, unclear active ingredients, large dosage, etc.

Inactive Publication Date: 2014-09-24
HEBEI YILING MEDICINE INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It has the functions of dispelling blood, dredging menstrual flow, dispelling blood stasis and eliminating symptoms. It is mainly used clinically to prevent and treat cardiovascular and cerebrovascular diseases and anti-cancer. Studies have shown that the main active ingredient of living leeches is hirudin, and the active ingredients in medicinal materials are still unclear. Therefore, at present Most of them are used as whole powder, which has the disadvantages of large dosage and easy to cause microbial contamination.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0076] The concrete preparation steps of leech pepsin hydrolyzate are as follows:

[0077] A, net selection leech 5g, be ground into fine powder;

[0078] B. Place the leech fine powder obtained in step A in a 250ml round-bottomed flask, then add 100ml of artificial gastric juice to the round-bottomed flask, then seal it in a constant temperature water bath at 43°C for 30 minutes, then add 2.5% pepsin for hydrolysis for 4 hours, then Put the enzymolysis solution in a constant temperature water bath at 85°C for 15 minutes, cool it to room temperature under running water, centrifuge at 4000r / min for 15 minutes, take the supernatant and dry it to obtain the leech pepsin hydrolyzate.

Embodiment 2

[0080] The concrete preparation steps of leech pepsin hydrolyzate are as follows:

[0081] A, net select leech 8g, be ground into fine powder;

[0082] B. Place the leech fine powder obtained in step A in a 250ml round bottom flask, then add 160ml of artificial gastric juice to the round bottom flask, then seal it in a constant temperature water bath at 43°C for 30 minutes, then add 2.5% pepsin for hydrolysis for 4 hours, then Put the enzymolysis solution in a constant temperature water bath at 85°C for 15 minutes, cool it to room temperature under running water, centrifuge at 4000r / min for 15 minutes, take the supernatant and dry it to obtain the leech pepsin hydrolyzate.

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PUM

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Abstract

The invention discloses a leech pepsin enzymolytic product and an application thereof. The leech pepsin enzymolytic product is obtained by selecting leeches, crushing into fine powder; adding the leech fine powder into artificial gastric juice, and adding pepsin for hydrolysis to obtain the leech pepsin enzymolytic product. The leech pepsin enzymolytic product has anticoagulation or thrombolytic effect.

Description

technical field [0001] The present invention relates to the anticoagulant and thrombolytic active ingredient of leech and its application. Background technique [0002] The leech (Hirudo) is the dried whole body of the leech Whitmania pigra Whitman, the leech Hirudo nipponica Whitman or the willow leaf leech Whitmania acranulata Whitman. Salty in nature, bitter, flat in taste, slightly poisonous, and returns to Liver Meridian. It has the functions of dispelling blood, dredging menstrual flow, dispelling blood stasis and eliminating symptoms. It is mainly used clinically to prevent and treat cardiovascular and cerebrovascular diseases and anti-cancer. Studies have shown that the main active ingredient of living leeches is hirudin, and the active ingredients in medicinal materials are still unclear. Therefore, at present The whole powder is mostly used as medicine, which has the disadvantages of large dosage and easy to cause microbial contamination. In view of the fact that...

Claims

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Application Information

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IPC IPC(8): A61K35/62A61P7/02
Inventor 李晓燕赵韶华王鑫国王玉蓉
Owner HEBEI YILING MEDICINE INST
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