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Breeding method of hybrid rice two-line sterile line with leaves carrying white midribs

A hybrid rice and sterile line technology, applied in the field of agriculture, can solve the problems of reducing the photosynthetic efficiency of rice, having a great impact on rice photosynthesis, affecting the growth period of rice sterile lines, and agronomic traits, and achieving good plant and leaf shape and agronomic traits Excellent, the effect of improving the purity of sterile lines and hybrids

Active Publication Date: 2014-09-24
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] However, it is worth noting that the existing leaf color markers such as green-turning albino leaf color, purple leaf, yellow leaf, light green and striped vein markers are expressed in large areas in leaves, which have a great impact on the photosynthesis of rice and reduce the The photosynthetic efficiency of rice, which more or less affects the growth period and agronomic traits of rice and even the cultivated sterile lines

Method used

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  • Breeding method of hybrid rice two-line sterile line with leaves carrying white midribs
  • Breeding method of hybrid rice two-line sterile line with leaves carrying white midribs
  • Breeding method of hybrid rice two-line sterile line with leaves carrying white midribs

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Embodiment Construction

[0028] The present invention will be further explained below in conjunction with specific examples.

[0029] Breeding material:

[0030] Y58S: The leaf midrib selected by the Hunan Hybrid Rice Research Center is a green two-line male sterile line Y58S (authorized number Xiangshendao 2005044, which can be purchased from Professor Deng Qiyun of Hunan Agricultural University).

[0031] Zhejiang white-veined rice: NG-BM-01, a mutant rice variety with white midribs selected from the mutagenic japonica rice "Ningjing 3", the application number is 20140427.5.

[0032] The breeding process of Zhejiang white veined rice is as follows:

[0033] (1) Get 3000 dry seeds of "Ningjing No. 3" with a moisture content of 13%, carry out cobalt-60 gamma ray mutagenesis, dose 250Gy, dose rate 10Gy / min, get mutagenic seeds (M 0 ).

[0034] (2) direct seeding mutation seeds, mixed with the seeds of all mutation plants (M 1 ).

[0035] (3) The seeds obtained in step (2) are planted and transplan...

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Abstract

The invention discloses a breeding method of a hybrid rice two-line sterile line with leaves carrying white midribs. The breeding method comprises the following steps: with Zhe midrib rice as a male parent and a rice variety of the two-line sterile line as a female parent, hybridizing to obtain F1-generation seeds; selfing the F1-generation seeds; performing back-crossing on fertile rice plants with excellent characters and white midribs, which are selected from an F2-generation plant, and the rice variety of the two-line sterile line to obtain BC1F1-generation seeds; performing back-crossing on an excellent fertile plant which is selected from the BC1F1-generation seeds, and the rice variety of the two-line sterile line to obtain BC2F1-generation seeds; repeating selfing and back-crossing, performing one-time selfing after continuous back-crossing for twice, and performing back-crossing for the last time; and selfing later generations obtained after the last-time back-crossing, selecting sterile single plants with leaves carrying white midribs and excellent characters from the selfed later generations, and developing breeding of a system to obtain the hybrid rice two-line sterile line. In the sterile line obtained by the invention, the marked characters are expressed in the midribs of the leaves only, without influencing the economical characters; hybrids can be simply rejected, and the purity can be identified.

Description

technical field [0001] The invention belongs to the field of agricultural technology, and in particular relates to a method for breeding two-line male sterile lines of hybrid rice with leaves carrying white midribs. Background technique [0002] The annual planting area of ​​hybrid rice in my country accounts for about half of the total planting area of ​​rice, especially the rapid development of two-line hybrid rice. However, it is worth noting that during the seed production of two-line hybrid rice, if there is continuous low temperature and rainy weather, there will often be a phenomenon of fertility conversion, resulting in a decrease in the purity of two-line hybrid rice seeds, which will bring hidden dangers to agricultural production safety. For this reason, breeders have designed to combine leaf color markers with breeding, introduce leaf color markers into sterile lines, and breed sterile lines with leaf color markers. strain. [0003] At present, the most widely ...

Claims

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Application Information

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IPC IPC(8): A01H1/02
Inventor 吴殿星舒小丽孙健王寅柳蕾
Owner ZHEJIANG UNIV
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