Method for preparing thrombin/tannic acid multilayer film compound chitosan hemostatic sponge
A technology of hemostatic sponge and chitosan, which is applied in medical science, bandages, absorbent pads, etc., can solve the problems of limited immobilization of porous sponges, and achieve the effects of good clinical application prospects, improved hemostasis performance, and improved storage stability.
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Embodiment 1
[0025] 1) Dissolve chitosan powder in acetic acid to prepare a chitosan solution with a mass concentration of 1%. After suction filtration, add excess sodium hydroxide aqueous solution until the gel is completely precipitated, and stir to obtain a suspension of gel particles. Rinse with water until neutral, add glycerol solution to plasticize, then centrifuge, pour into a mold, let stand for defoaming, freeze and then freeze-dry to obtain chitosan porous sponge;
[0026] 2) Sterilize the chitosan sponge obtained by freeze-drying by ultraviolet irradiation;
[0027] 3) Dissolve BPEI in phosphate buffered solution PBS, and prepare a BPEI solution with a mass concentration of 1 mg / mL, which is called solution A; dissolve tannic acid in PBS, and prepare a tannic acid solution with a mass concentration of 1 mg / mL, which is called Liquid B: Dissolve thrombin in PBS to prepare a thrombin solution with a mass concentration of 1 mg / mL, called liquid C.
[0028] 4) Soak the chitosan sp...
Embodiment 2
[0036] 1) Dissolve chitosan powder in acetic acid to prepare a chitosan solution with a mass concentration of 3%. After suction filtration, add excess sodium hydroxide aqueous solution until the gel is completely precipitated. Stir to obtain a suspension of gel particles. Rinse with water until neutral, add glycerol solution to plasticize, then centrifuge, pour into a mold, let stand for defoaming, freeze and then freeze-dry to obtain chitosan porous sponge;
[0037] 2) Sterilize the chitosan sponge obtained by freeze-drying by ultraviolet irradiation;
[0038] 3) Dissolve BPEI in PBS, and prepare a BPEI solution with a mass concentration of 2 mg / mL, which is called solution A; dissolve tannic acid in PBS, and prepare a tannic acid solution with a mass concentration of 2 mg / mL, which is called solution B; Dissolve thrombin in PBS, and prepare a thrombin solution with a mass concentration of 2 mg / mL, which is called solution C.
[0039] 4) Soak the chitosan sponge in step 2) i...
Embodiment 3
[0046]1) Dissolve chitosan powder in acetic acid to prepare a chitosan solution with a mass concentration of 5%. After suction filtration, add excess sodium hydroxide aqueous solution until the gel is completely precipitated, and stir to obtain a suspension of gel particles. Rinse with water until neutral, add glycerol solution to plasticize, then centrifuge, pour into a mold, let stand for defoaming, freeze and then freeze-dry to obtain chitosan porous sponge;
[0047] 2) Sterilize the chitosan sponge obtained by freeze-drying by ultraviolet irradiation;
[0048] 3) Dissolve BPEI in PBS, and prepare a BPEI solution with a mass concentration of 3 mg / mL, which is called liquid A; dissolve tannic acid in PBS, and prepare a tannic acid solution with a mass concentration of 3 mg / mL, which is called liquid B; Dissolve thrombin in PBS, and prepare a thrombin solution with a mass concentration of 3 mg / mL, which is called solution C.
[0049] 4) Soak the chitosan sponge in step 2) in...
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