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Preparation method of tubular silk fibroin/keratin composite nanofiber material

A composite nanofiber, silk fibroin technology, applied in cellulose/protein conjugated rayon, fiber processing, filament/thread forming, etc., can solve the problem of low molecular weight of keratin, application limitation, low mechanical strength, etc. problem, to achieve the effect of good biocompatibility, lower mechanical properties, and adjustable inner diameter

Inactive Publication Date: 2014-09-03
DONGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, existing studies have proved that due to the low molecular weight of keratin, the membrane material made of a single keratin material is usually brittle and has low mechanical strength, which limits its application.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] The keratin was extracted from wool by reduction method. The specific method was: weigh 5g of wool, use petroleum ether as solvent to remove the oil on the surface of wool by Soxhlet extraction, then wash the wool with ethanol and air-dry it. Add the above-mentioned wool into 100ml, 1mol / l mercaptoethanol, adjust the pH to about 10.0 with NaOH, and then react at 40°C for 12h. After the reaction was finished, the reaction solution was collected (referred to as filtrate 1), and the wool solid remaining in the reaction was collected by filtration, and continued to be treated with 100ml, 0.1mol / l Tris lye at 40°C for 2h, and then filtered to remove the solid. The filtrate was collected (designated as filtrate 2). .The filtrates 1 and 2 were combined, centrifuged at 6000rpm for 5 minutes, and then dialyzed with a dialysis bag with a molecular weight cut-off of 40,000 for 36 hours. Finally, the dialysate was added to liquid nitrogen for quick freezing, and then dried in a fre...

Embodiment 2

[0029] Using human hair as raw material to extract keratin by reduction method, the specific method is: weigh 5g of human hair, use petroleum ether as solvent to remove human hair surface oil by Soxhlet extraction, then wash human hair with ethanol and air-dry. Add the above-mentioned human hair into a mixed solution of 200ml of water and 5g of sodium metabisulfite, adjust the pH to about 10.0 with NaOH, and then react at 60°C for 5h. After the reaction, collect the reaction solution (referred to as filtrate 1), and collect the remaining human hair solid by filtration at the same time, continue to treat it with 100ml, 0.1mol / l Tris lye at 40°C for 2h, and then filter to remove the solid , collect the filtrate (referred to as filtrate 2). .The filtrates 1 and 2 were combined, centrifuged at 6000rpm for 5 minutes, and then dialyzed with a dialysis bag with a molecular weight cut-off of 8,000 for 36 hours. Finally, the dialysate was added to liquid nitrogen for quick freezing, an...

Embodiment 3

[0033]The keratin is extracted from wool by oxidation method. The specific method is: weigh 5g of wool, use petroleum ether as solvent to remove the oil on the surface of wool by Soxhlet extraction, then wash the wool with ethanol and air-dry it. 100ml of deionized water and 5ml of 30% hydrogen peroxide were added to the wool, and then reacted at 100°C for 2h. After the reaction was finished, the reaction solution was collected (referred to as filtrate 1), and the wool solid remaining in the reaction was collected by filtration, and continued to be treated with 100ml, 0.1mol / l Tris lye at 40°C for 2h, and then filtered to remove the solid. The filtrate was collected (designated as filtrate 2). .The filtrates 1 and 2 were combined, centrifuged at 6000rpm for 5 minutes, and then dialyzed with a dialysis bag with a molecular weight cut-off of 8,000 for 36 hours. Finally, the dialysate was added to liquid nitrogen for quick freezing, and then dried in a freeze dryer to obtain dry ...

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PUM

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Abstract

The invention relates to a preparation method of a tubular silk fibroin / keratin composite nanofiber material. The preparation method comprises the steps of adding silk fibroin into a solvent and stirring until dissolving completely to obtain silk fibroin solution with the concentration of 5-20mg / ml; adding keratin into the solvent and stirring until dissolving completely to obtain keratin solution with the concentration of 10-20mg / ml; mixing the silk fibroin solution with the keratin solution in a mass ratio of 95:5-5:95, stirring to obtain a spinning solution, then performing electrostatic spinning and using a cylindrical collecting roller as a receiver. The tubular material has the advantages of adjustable inside dimension, good biocompatibility and in-vivo degradation, and is expected to be used in the field of biological medical materials such as artificial blood vessels and urethra substitutions.

Description

technical field [0001] The invention belongs to the field of preparation of nanofiber tubular materials, in particular to a preparation method of silk fibroin / keratin composite nanofiber tubular materials. Background technique [0002] Keratin is an insoluble fibrous animal protein, a structural protein of ectodermal cells, widely present in animal skin and skin appendages, such as hair, hooves, shells, claws, horns, scales, etc. A large number of studies in recent years have shown that keratin is a high-quality biomedical material with good biocompatibility and not rejected by the body's immune system, and has broad application prospects. Most prominently, the amino acid sequence of keratin derived from wool and other sources was found to contain the same Arg-Gly-Asp (RGD) tripeptide sequence. This tripeptide sequence is recognized as an effective binding site for cell binding in the extracellular matrix, and has the function of promoting cell adsorption. Therefore, a lar...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): D01F8/02D01D5/00
Inventor 杨光
Owner DONGHUA UNIV
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