Model making method of bacterial biofilm-related nasosinusitis animal model
A bacterial biofilm and correlation technology, applied in the field of biomedicine, can solve the problems of unsuitable scientific research, difficult operation, high price, etc., achieve little impact on normal structure and function, strong operability, and improve the success rate of modeling Effect
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0046] (1) Screening for BBF-positive Staphylococcus aureus:
[0047] Collect clinical specimens, sort and identify Staphylococcus aureus by conventional methods, pick a single colony and inoculate into TSB culture solution (TSBg) containing 0.5% glucose, shake overnight at 37°C and dilute 200 times with TSBg, add polystyrene material In a 96-well plate, 200uL per well, cultured at 37°C for 24 hours, washed with PBS to remove planktonic bacteria, fixed with methanol for 15 minutes, air-dried for 30 minutes after aspirating the methanol, added 200uL of 1% crystal violet solution, stained for 15 minutes, and rinsed thoroughly with PBS Add 200 uL of 95% ethanol to each well of the unbound crystal violet staining solution after drying, place it on a shaker at room temperature for 1 hour, and measure the absorbance (OD value) at 490 nm with a microplate reader. OD value ≥ 0.12 is considered to be a BBF-positive strain, and the larger the OD value, the stronger the ability to form B...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com