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Novel applications of materials for detecting nucleotides of rs7597593 site and rs1344706 site

A nucleotide and site technology, applied in the field of biomedicine where the invention belongs to, can solve the problem of unclear exact function of the ZNF804A gene, and achieve the effect of preventing heroin addiction diseases and great application value

Active Publication Date: 2014-08-27
PEKING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Currently, the exact function of the ZNF804A gene is unknown

Method used

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  • Novel applications of materials for detecting nucleotides of rs7597593 site and rs1344706 site
  • Novel applications of materials for detecting nucleotides of rs7597593 site and rs1344706 site
  • Novel applications of materials for detecting nucleotides of rs7597593 site and rs1344706 site

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1. Detecting the nucleotides at the rs7597593 site and the rs1344706 site in a subject, and obtaining the joint genotype of the subject population based on the rs7597593 site and the rs1344706 site

[0031] 1. Extract the genomic DNA from the peripheral venous blood of the subjects.

[0032] 2. Using the genomic DNA extracted in step 1 as a template, PCR amplification was performed using a specific primer pair composed of rs7597593-upper and rs7597593-lower to obtain a PCR amplification product.

[0033] 3. Sequencing the PCR amplification product in step 2, so as to know the genotype of the subject based on the rs7597593 site. The sequencing results showed that the PCR amplification products were all the target sequences in the ZNF804A gene, and there was no other non-specific amplification, that is, for the specific primer pair, there were no other amplified targets in the human genome, and the specificity good.

[0034] 4. Using the genomic DNA extracted in...

Embodiment 2

[0046]Example 2, Edward Gambling Test (Iowa Gambling Task, IGT)

[0047] Using the IGT classic card method, there are four groups of cards (A, B, C, D), each group has 40 cards, stacked according to certain rules. Among them, Group A and Group B represent high-risk cards, with high scores and higher loss points. The more you choose these two groups of cards, the lower the final total score; Group C and D are low-risk cards with low scores. There are fewer points lost, and the more cards you choose from these two groups, the higher your final total score. In the case of not knowing the score of each card in advance, the subject chooses one card from the four groups of cards at a time according to the instruction to maximize the total score, and stops when a total of 100 cards are selected. Finally, the result of selecting the total number of low-risk Group C and D cards minus the total number of high-risk Group A and B cards ((C+D)-(A+B)) represents the impulsive decision-maki...

Embodiment 3

[0053] Example 3. Combining genotypes with changes in brain images of subjects

[0054] In the Department of Imaging, Zhongshan Hospital of Traditional Chinese Medicine, Guangdong Province, a magnetic resonance scanner was used to scan the subjects' heads. Fix the subject's head with a matching foam pad, plug the ears with rubber plugs to reduce noise interference, and keep the subject at rest throughout the scanning process, lying quietly on his back with his eyes closed. A total of 2 different MR sequences (3D-T1; T2) were scanned, and each sequence was repeated. First complete the scan of the positioning image, and then perform the axial 3D-T1 spoiled gradient echo sequence (SPGR) scan, the scan parameters are: slice thickness = 1 mm, repetition time (TR) = 7.816 milliseconds, echo time (TE) = 2.984 milliseconds, inversion time = 450ms, inversion angle = 13°, matrix = 256×256, field of view (FOV) = 256×256mm 2 , the average number of times = 2. During the scanning proces...

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Abstract

The invention discloses novel applications of materials for detecting nucleotides of a rs7597593 site and a rs1344706 site in ZNF804A genes. The applications of the material for detecting the nucleotide of the rs7597593 and the material for detecting the nucleotide of the rs1344706 site in preparation of a kit for evaluating the risk of heroin addiction need to be protected. The combined genes of the rs7597593 site and the rs1344706 site are divided into risk-type genes, intermediate genes and protective genes. Based on the combined genes of the rs7597593 site and the rs1344706 site, a tested person with the risk-type genes has the high risk of the heroin addiction; the tested person with the protective genes has the low risk of the heroin addiction. The applications of the materials, which are disclosed by the invention, have significant application values favorable for prevention of heroin addiction diseases, prediction and evaluation of disease processes of heroin addicts, effective intervention of the heroin addicts and development of novel medicines aiming at the heroin addicts.

Description

technical field [0001] The invention belongs to the field of biomedicine, and relates to a new application of substances for detecting nucleotides of rs7597593 and rs1344706 in the ZNF804A gene, specifically for detecting rs7597593 and rs1344706 in the ZNF804A gene Nucleotide substances are used in the preparation of kits for evaluating the risk of heroin addiction and / or the degree of impulsive decision-making behavior impairment of heroin addicts and / or the gray matter volume of the left sensorimotor cortex of the brain of heroin addicts new use. Background technique [0002] Substance addiction, also known as substance dependence, refers to a strong and irresistible state of looking for the substance psychologically and physically after long-term abuse of a substance, in order to experience the euphoria of repeated use of the substance. At the same time avoid physical discomfort caused by withdrawal. Substance addiction disorder is a recurrent chronic recurrent encephal...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6883C12Q2600/156
Inventor 时杰孙艳胡蝶梁洁陆林王贵彬朱维莉
Owner PEKING UNIV
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