Immobilized microorganism oil-spilling repairing agent and preparation method

A technology for immobilizing microorganisms and repairing agents, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of difficulty in forming polyvinyl alcohol balls, reducing microbial activity, and low mechanical strength of preparations, achieving high Microbial activity and cell capacity, enhanced tolerance, good stability and reusability

Inactive Publication Date: 2014-08-27
ZHEJIANG OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Compared with sodium alginate-CaCl2 embedding method, polyvinyl alcohol-H 3 BO 3 Although the prepared immobilized preparation has high mechanical strength and long service life, it is difficult for polyvinyl alcohol to form into balls due to strong agglomeration, and the saturated boric acid solution used for cross-linking polyvinyl alcohol is highly toxic to microorganisms and will Significantly reduces the activity of microorganisms after immobilization
Sodium Alginate-CaCl 2 Although the embedding method has mild reaction conditions, the embedding material is non-toxic to organisms, and the embedded cells have high activity, the mechanical strength of the preparation is low and the mass transfer is poor.

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0040] A preparation method of an immobilized microbial oil spill restoration agent using sodium alginate-calcium chloride-modified mussel shells as an embedded and immobilized material, the specific steps are:

[0041] 1. Preparation of modified mussel shells

[0042] 1) Cleaning, sieving, drying and pulverizing the raw mussel shells, passing through an 80-mesh sieve, and setting aside;

[0043] 2) Weigh the mussel shells crushed above, and add the citric acid solution with a mass fraction of 2.5%, wherein the mass ratio of the mussel shells to the citric acid solution is 1:2, stir and mix, and mix the mixed feed solution in Immerse at room temperature for about 1.5h;

[0044] 3) Put the impregnated material liquid into a high-temperature muffle furnace, raise it from room temperature to the required activation temperature of 550° C., and keep the temperature at a rate of 50° C. / min for 30 minutes.

[0045] 4) The activated sample is taken out from the muffle furnace and co...

Embodiment 2

[0056] A preparation method of an immobilized microbial oil spill restoration agent using sodium alginate-calcium chloride-modified mussel shells as an embedded and immobilized material, the specific steps are:

[0057] 1. Preparation of modified mussel shells

[0058] 1) Cleaning, screening, drying and pulverizing the raw mussel shells, passing through a 40-mesh sieve, and setting aside;

[0059] 2) Take the mussel shells that have been crushed, and add them to a citric acid solution with a mass fraction of 5%, wherein the mass ratio of the mussel shells to the citric acid solution is 1:1, stir and mix, and put the mixed feed solution in Immerse at room temperature for about 2.0h;

[0060] 3) Put the impregnated material liquid into a high-temperature muffle furnace, raise it from room temperature to the required activation temperature of 300° C., and keep the temperature at a rate of 60° C. / min for 30 minutes.

[0061] 4) The activated sample is taken out from the muffle f...

Embodiment 3

[0072] A preparation method of an immobilized microbial oil spill restoration agent using sodium alginate-calcium chloride-modified mussel shells as an embedded and immobilized material, the specific steps are:

[0073] 1. Preparation of modified mussel shells

[0074] 1) Cleaning, sieving, drying and pulverizing the raw mussel shells, passing through an 80-mesh sieve, and setting aside;

[0075] 2) Take the crushed mussel shells and add them to a citric acid solution with a mass fraction of 2%, wherein the mass ratio of the mussel shells to the citric acid solution is 1:3, stir and mix, and put the mixed feed solution in Immerse at room temperature for about 1.0h;

[0076] 3) Put the impregnated material liquid into a high-temperature muffle furnace, raise it from room temperature to the required activation temperature of 600° C., and keep the temperature at a rate of 50° C. / min for 15 minutes.

[0077] 4) The activated sample is taken out from the muffle furnace and cooled...

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PUM

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Abstract

The invention discloses an immobilized microorganism oil-spilling repairing agent. The immobilized microorganism oil-spilling repairing agent has an embedded microorganism concentration of 5*10<7> to 2*10<8> cell / g, and has carrier ingredients of mussel shell, sodium alginate and CaCl2. The immobilized microorganism oil-spilling repairing agent has a petroleum hydrocarbon degradation rate of 82-94% under the conditions of salinity of 5-55 parts per thousand, a temperature of 10-30 DEG C and a pH value of 6.5-9.0. Compared with free microorganisms, the immobilized microorganism oil-spilling repairing agent has a degradation rate improved by 39-47% and has improved environmental tolerance.

Description

technical field [0001] The invention relates to a bioremediation technology for petroleum product pollution. Background technique [0002] With the development of offshore oil development and marine transportation, oil spill accidents caused by blowouts, oil spills from transport ships, shipwrecks, and oil pipeline leaks have occurred frequently. After the oil spill accident, although some surface oil can be removed by physical methods (such as fence recovery, etc.) and chemical methods (spraying dispersant, etc.), the remaining petroleum hydrocarbons remain in the marine environment for a long time, which is harmful to marine ecosystems and Human health will cause huge lasting damage (Pelletier E, Delille D, Delille B. Crude oil bioremediation in sub-Antarctic intertidal sediments: chemistry and toxicity of oiled residues [J]. Mar. Environ. Res., 2004, 57( 4): 311-327.). [0003] At present, the biological remediation technology of oil pollution is mainly based on microbi...

Claims

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Application Information

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IPC IPC(8): C12N11/14C12N11/10C02F3/34C01B31/08C12R1/01C02F101/32C02F103/08C01B32/324
Inventor 陈庆国刘梅孙静亚穆军
Owner ZHEJIANG OCEAN UNIV
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