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Decellularized method for cornea

A technology of decellularization and cornea, applied in the field of medical devices, can solve the problems of surfactant residue, failure to achieve biocompatibility, toxicity and other problems, and achieve the effect of mild reagents

Active Publication Date: 2014-08-27
SHENZHEN AINEAR CORNEA ENG
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Problems solved by technology

[0003] However, in the decellularization process of the acellular corneal stroma produced by the prior art, solutions such as trypsin, EDTA (ethylenediaminetetraacetic acid) and Triton-X100 (polyethylene glycol octylphenyl ether), deoxycholorin Sodium dodecyl sulfate, SDS (sodium dodecylsulfonate) and other surfactants, although these methods can remove cell components, but the reaction is violent, and the damage to the corneal stroma is serious. Among them, the use of trypsin treatment can easily lead to the degradation of the corneal stroma. Active agents can easily lead to surfactant residues, resulting in cytotoxicity, which can easily cause toxic reactions after transplantation, and cannot achieve good biocompatibility

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Embodiment Construction

[0024] It should be understood that the specific implementation manner described here is the best implementation manner for realizing the purpose of the present invention, and it is only used to explain the present invention, and is not intended to limit the present invention.

[0025] The present invention provides a method for decellularization of the cornea. In the first embodiment, the method for decellularization of the cornea comprises the following steps:

[0026] A. Place the extracted animal cornea in water, then shake it in a constant temperature oscillator for 5 hours (hours) at a speed of 200 rpm (rotation per minute), during which the water is changed every 1 hour to make the epithelial layer come off. The animals include pigs, cattle, sheep and other animals, and the water used includes injection water, pure water, physiological saline and the like. During the shaking treatment, the collagen in the corneal stroma absorbs water and fluffs up, the cornea gradually ...

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Abstract

The invention discloses a decellularized method for cornea. The decellularized method includes the following steps that (A), the picked animal cornea is placed in water, vibration treatment is carried out, and an epithelial layer falls off accordingly; (B), the cornea is placed in decellularized reagents for vibration treatment; (C), the cornea is placed in water for vibration treatment; (D), the step B and the step C are repeated alternately so that stroma cells and endothelial cells can fall off, wherein the decellularized reagents contain NaCl and EDTA. According to the decellularized method, the epithelial layer of the cornea and immunogen cellular constituents can be removed rapidly and tenderly, and therefore undamaged decellularized cornea ground substances can be obtained and have good biocompatibility and anti-degradation capacity.

Description

technical field [0001] The invention relates to the field of medical devices, in particular to a corneal decellularization method. Background technique [0002] Corneal disease is the second leading cause of blindness in the world, and the number of cases is increasing at a rate of 1.5 to 2 million per year. Corneal transplantation is currently the only effective way to treat corneal blindness, but the extreme scarcity of donor corneas restricts the development of corneal transplantation. Corneal stroma The corneal stroma of animals, such as animals, has similar tissue structure, biophysical properties and optical properties to human corneal stroma, but the strong rejection after xenotransplantation hinders the clinical application of xenogeneic corneas. Studies in recent years have found that the stromal cells in the corneal stroma are the main antigens that cause stromal rejection, and the collagen fibers that are the corneal stroma structure are highly conserved among sp...

Claims

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Application Information

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IPC IPC(8): A61L27/38A61L27/24
Inventor 王维博张斌
Owner SHENZHEN AINEAR CORNEA ENG
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