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A kind of rapid determination method of coumarin and safrole in essence and fragrance

A rapid detection technology for flavors and fragrances, which is applied in the field of analysis to achieve the effects of fast analysis, protection of chromatographic columns, and guaranteed extraction efficiency

Active Publication Date: 2015-11-18
CHINA TOBACCO GUANGXI IND
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  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is no report on the simultaneous determination of safrole and coumarin in flavors and fragrances by ultra-high performance liquid chromatography-tandem mass spectrometry

Method used

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  • A kind of rapid determination method of coumarin and safrole in essence and fragrance
  • A kind of rapid determination method of coumarin and safrole in essence and fragrance
  • A kind of rapid determination method of coumarin and safrole in essence and fragrance

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Embodiment 1

[0029] Instruments and reagents: Agilent1290 ultra-high performance liquid chromatography (Agilent, USA), API3200 triple quadrupole tandem mass spectrometer (AB, USA), multifunctional automatic oscillator, analytical balance (sensitivity 0.1 mg).

[0030] Coumarin, Coumarin-d 4 Purchased from Dr. Ehrenstorfer GmbH, purity ≥ 98%; safrole was purchased from Dima Company, purity ≥ 97%; acetonitrile was chromatographically pure, water was deionized water, and other reagents were analytically pure.

[0031] Weigh 0.01g of coumarin and 0.01g of safrole, accurate to 0.0001g, dilute to 500mL with 60% ethanol aqueous solution, and then prepare a mixed standard solution containing 5 μg / mL of safrole and coumarin by dilution; weigh a certain amount Coumarin-d 4 , Prepare an internal standard solution with a concentration of 125 μg / mL with 60% ethanol aqueous solution.

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Abstract

The invention discloses a quick measurement method for coumarin and safrole in essence and flavor. The quick measurement method comprises the steps of performing oscillation extraction on an essence and flavor sample by taking ethyl alcohol aqueous solution as a solvent, filtering an extracting solution through an organic filtering film, and measuring the coumarin and the safrole through ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS / MS). The quick measurement method has the characteristics that difficulties in quantified separation and purification of a complicated mixture system are avoided through quantification by an isotope dilution internal standard method; the ethyl alcohol aqueous solution is used as the sample extraction solvent, so that the extraction efficiency is guaranteed, and the amount of organic impurities, which enter a chromatographic column, in small samples is reduced; furthermore, the chromatographic column is effectively protected; compared with the conventional method, the UPLC-MS / MS is high in analysis speed, high in repetitiveness and high in recycling rate, and is suitable for analysis of a large batch of samples.

Description

technical field [0001] The invention belongs to the technical field of analysis methods, in particular to a method for determining the content of coumarin and safrole in flavors and fragrances for tobacco. Background technique [0002] The U.S. Food and Drug Administration (FDA) banned the addition of coumarin to food as early as 1954, and the British Food Standardization Committee also listed coumarin as an additive prohibited from being used in food in 1965. It is used as a food additive. Safrole can form safrole-deoxyribonucleic acid (DNA) adducts in liver tissue. When the content of safrole in food is more than 0.5%, it will cause hepatoma and has carcinogenic effect. It is a strong carcinogen of digestive system, blood system and urinary system. substances, the use of which is prohibited in countries all over the world. In the production process of flavors and fragrances, it is possible to introduce components such as safrole and coumarin from natural plant essential ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/88
Inventor 范忠黄善松黄世杰朱静冯守爱王维刚周艳枚陆冰琳蒋宏霖周奕
Owner CHINA TOBACCO GUANGXI IND
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