A kind of tetrodotoxin positive quality control sample and its preparation method and application
A technology of tetrodotoxin and positive quality control, applied in the field of food safety testing, to achieve the effect of long storage time and simple preparation
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Embodiment 4
[0035] The additive formula is C 18 Powder, additive formula is C 18 , The ratio of graphitized carbon and diatomaceous earth is 2:2;1, but not limited thereto.
Embodiment 1
[0037] Sample pretreatment
[0038] 1) Live pufferfish were killed by freezing, peeled off the skin, and the muscle part was taken to remove tendons and thoroughly pulverized by a homogenizer.
[0039] 2) Weigh (1.00±0.01) g of the sample into a 50 mL polypropylene centrifuge tube, accurately add 5 mL of 1% acetic acid methanol (1:99, v / v) solution, homogenize for 30 s, and ultrasonically extract at 50 ° C for 25 min.8000r Centrifuge at / min*5min, transfer the supernatant to another clean centrifuge tube; repeat the extraction of the residue once with 4mL 1% acetic acid methanol solution, combine the supernatant and centrifuge at 12000r / min at 4°C for 5min, the obtained supernatant is to be purified ;
[0040] 3) Take 1.0mL supernatant to a 2mL EP (Eppendorf) tube, add 100mg graphitized carbon black (GCB) and 100mg C 18 The powder was vigorously vortexed for 30s, centrifuged at 10000r / min for 5min, and the supernatant was filtered through a 0.22μm polytetrafluoroethylene-nyl...
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