Fluorescent quantitative PCR detection kit for infectious spleen and kidney necrosis virus and Fluorescent quantitative PCR detection method of infectious spleen and kidney necrosis virus
A spleen and kidney necrosis virus, detection kit technology, applied in the directions of microorganism-based methods, biochemical equipment and methods, microbial determination/inspection, etc., to achieve the effect of saving costs and simplifying operating procedures
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment
[0038] 1. Design of specific primers and probes
[0039] According to the ORF007 protein nucleic acid sequence (NC_003494) retrieved from Genbank, primers and probes were designed for the detection of Siniperca ISPKV in the conserved gene fragment by blast comparison. The sequence is as follows:
[0040] ISKNV - F: 5'-CGAGGCCACATCCAACATC-3' (SEQ ID NO: 1);
[0041] ISKNV - R: 5'-CGCCTTTAACGTGGGATATATTG-3' (SEQ ID NO: 2);
[0042] ISKNV-P: 5'-FAM-CACCAAACTGACCGCGGACTCGT-Eclipse-3' (SEQ ID NO: 3);
[0043] The fluorescent probe reporter group is FAM, and the quencher group is Eclipse.
[0044] 2. Preparation of positive standard
[0045] Extract the nucleic acid sequence of mandarin fish infectious spleen and kidney necrosis virus ORF007 protein, and insert it into the T vector by conventional methods, which is the positive standard.
[0046] 3. Extraction of viral DNA
[0047] According to the instructions of Qiagen Blood Tissue DNA Extraction Kit (purchased from Qiagen, ...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com