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A kind of liquid state fermentation production method of probiotic preparation mainly producing cellulase for feeding

A technology of probiotic preparation and cellulase, which is applied in application, animal feed, animal feed, etc., can solve the problems of joint application of microbial cells, unfavorable product quality control, and extensive cultivation process, etc., to increase the number and The effect of product flavor, improving economic and social benefits, and reducing recovery and purification costs

Active Publication Date: 2016-06-08
阳江安辰蛋白生物科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Bacillus can produce various enzymes such as cellulase (a method for preparing cellulase using Bacillus, Chinese patent: CN102766615B; cellulase-producing strain, cellulase and its production and fermentation method, Chinese patent: CN102586133A), but the above-mentioned documents are all methods of producing cellulase by using a single bacterium, and there is no mention of the effective method of combined application of microbial cells and cellulase after fermentation, especially as a feed additive
Invention patent applications 201110389177.1 and 201110268139.0 involve multi-bacteria fermentation to produce feed probiotics, but they both use relatively simple solid-state accumulation fermentation process, the cultivation process is extensive, easy to contaminate miscellaneous bacteria, which is not conducive to product quality control, and does not focus on cellulose Enzyme products
Invention patent application 201310422513.7 relates to the production method of probiotic feed containing various enzymes, but the enzymes are derived from artificial additions in the production process, rather than produced by the strain itself in the fermentation process, which is equivalent to a disguised increase in production costs

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] A liquid fermentation production method mainly producing cellulase feed probiotic preparations, comprising the following steps:

[0030] (1) Preparation of fermentation strains: the strains are Bacillus subtilis, Bacillus licheniformis and Saccharomyces cerevisiae. The strains stored on the slant of the test tube were transferred to the activation medium, and cultured on a rotary shaker at 28°C for 35 hours to the logarithmic growth phase of the strains. Bacillus activation medium (g / L): yeast powder 5, peptone 10, sodium chloride 10, pH7.0; Saccharomyces cerevisiae activation medium (g / L): yeast powder 2, peptone 10, glucose 20, pH6.5; the activated medium was sterilized at 115°C for 20 minutes before use;

[0031] (2) Liquid medium for fermentation (g / L): 10 dried crushed beer grains, 50 cornmeal, 20 glucose, 12 peptone, 6 yeast powder, 4 dipotassium hydrogen phosphate, 4 sodium dihydrogen phosphate, and sulfuric acid heptahydrate Magnesium 1, pH 7.3;

[0032] (3) ...

Embodiment 2

[0039] A liquid fermentation production method mainly producing cellulase feed probiotic preparations, comprising the following steps:

[0040] (1) Preparation of fermentation strains: the fermentation strains are Bacillus pumilus, Bacillus licheniformis and Saccharomyces cerevisiae. The strains stored on the slant of the test tube were transferred to the activation medium, and cultivated on a rotary shaker at 32°C for 20 hours to the logarithmic growth phase of the strains. Bacillus activation medium (g / L): yeast powder 6, peptone 12, sodium chloride 8, pH7.0; Saccharomyces cerevisiae activation medium (g / L): yeast powder 4, peptone 10, glucose 25, pH6.5; the activated medium was sterilized at 115°C for 20 minutes before use;

[0041] (2) Liquid medium for fermentation (g / L): 20 dry crushed brewer's grains, 40 cornmeal, 10 glucose, 15 peptone, 5 yeast powder, 5 dipotassium hydrogen phosphate, 5 sodium dihydrogen phosphate, and sulfuric acid heptahydrate Magnesium 1.2, pH 7....

Embodiment 3

[0049] A liquid fermentation production method mainly producing cellulase feed probiotic preparations, comprising the following steps:

[0050] (1) Preparation of fermentation strains: the fermentation strains are Bacillus subtilis, Bacillus pumilus and Saccharomyces cerevisiae. The strains stored on the slant of the test tube were transferred to the activation medium, and cultivated on a rotary shaker at 30°C for 25 hours to the logarithmic growth phase of the strains. Bacillus activation medium (g / L): yeast powder 8, peptone 12, sodium chloride 6, pH7.0; Saccharomyces cerevisiae activation medium (g / L): yeast powder 6, peptone 12, glucose 25, pH6.5; the activated medium was sterilized at 115°C for 20 minutes before use;

[0051] (2) Liquid medium for fermentation (g / L): 12 dried crushed beer grains, 55 cornmeal, 15 glucose, 10 peptone, 8 yeast powder, 3 dipotassium hydrogen phosphate, 3 sodium dihydrogen phosphate, and heptahydrate sulfuric acid Magnesium 2, pH 6.5;

[00...

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PUM

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Abstract

The invention discloses a fermentation production method of a feed probiotic preparation which is mainly used for producing cellulose. The liquid-state fermentation production method comprises the steps of with a liquid-state fermentation process, carrying out mixed flora combined fermentation by bacillus and brewer yeast to produce the feed probiotic preparation, and adding auxiliary materials and drying in the later period of fermentation. The feed probiotic preparation prepared by the method is provided with high-activity cellulase, and is endowed with activity of various digestive enzymes, the two functions of a feed cellulose preparation and a probiotic preparation are combined, environmental pollution is avoided, and the cost for subsequent recovery and purification of enzymic preparations is lowered; the efficiency is high, the palatability of animals for a feed product is improved, the absorption of animals for the nutrition of the feed is promoted, large-scale production and popularization are promoted, and a function of effectively promoting the healthful breeding in the livestock and poultry industry is achieved.

Description

Technical field: [0001] The invention belongs to the technical field of microbial feed additives in the field of biological feed, and relates to a liquid-state fermentation production method of a probiotic preparation for feed with the main purpose of producing cellulase. Background technique: [0002] As a green feed additive, cellulase can degrade cellulose in the feed into digestible and absorbable reducing sugars (such as disaccharides or glucose) to improve feed utilization. Adding cellulase can supplement the deficiency of endogenous enzymes in rearing animals, dissolve plant cell walls to promote absorption; reduce the viscosity of the gastrointestinal tract of animals, eliminate anti-nutritional factors; reduce the attachment and growth of harmful microorganisms; maintain the integrity of small intestinal villi, etc. Therefore, cellulase, as a highly active biological additive, has broad application prospects in the field of animal feed. [0003] Probiotics refer to...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23K20/189A23K10/18
Inventor 程仕伟崔海洋
Owner 阳江安辰蛋白生物科技有限公司
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