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Atractylis lancea tissue culturing and rapid propagating method

A technology of tissue culture rapid propagation and herb atractylodes, applied in horticultural methods, botany equipment and methods, horticulture, etc., can solve the problems of easy degradation of quality, poor seed vitality, low seed reproduction coefficient, etc., and achieve the effect of alleviating the contradiction between supply and demand

Active Publication Date: 2014-08-06
JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Artificial propagation of Atractylodes atractylodis can be propagated by both seeds and rhizomes, but the propagation coefficient of seed propagation is too low, and the vitality of seeds is poor; propagation by rhizomes requires a lot of rhizome materials, and the quality is easy to degrade

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Embodiment 1: a kind of method for tissue culture and rapid propagation of Atractylodes atractylodes, comprising the following steps:

[0025] 1. Seed treatment and disinfection

[0026] Put a filter paper of appropriate size in the petri dish, and select the large and plump wild Atractylodes atractylodis harvested in autumn ( Atractylodes lancea (Thunb.)DC.) seeds, washed with washing powder, and then soaked in appropriate amount of water (just below the seeds) for 0.5h; after soaking, rinsed repeatedly with running water, and then rinsed twice with distilled water; The seeds were soaked in 70% ethanol by volume for 30 seconds, then soaked in 0.1% mercuric chloride solution by mass for 15 minutes, rinsed three times with sterile water, and dried on sterile filter paper for later use.

[0027] 2. Establishment of sterile culture system

[0028] Put the above-mentioned sterilized seeds into the germination medium, 1 grain per bottle; germinate after 14 days, and grow i...

Embodiment 2

[0041] Embodiment 2: basically the same as Embodiment 1, the difference is:

[0042] The set culture conditions involved in steps 2 to 6 are as follows: light intensity: 2500lx; light time: 15h / d; culture temperature: 24°C.

[0043]The disinfection and sterilization treatment method in step (1) is: soak the cleaned seeds in clean water for 1 hour. After soaking, rinse them repeatedly with running water, then rinse them twice with distilled water, and then soak them in an ethanol solution with a volume percentage of 70%. 20s, then soaked in 0.1% mercuric chloride solution for 18 minutes, and finally rinsed with sterile water 5 times, blotted dry on sterile filter paper for later use.

[0044] In step (2), germinate under the set culture conditions for 10 days, and the pH of the germination medium is 6.2.

[0045] The callus induction and differentiation medium in step (3) is: MS+0.5mg / L 6-benzylaminoadenine+0.05mg / L naphthaleneacetic acid+3% sucrose+mass percent 0.75% Agar, p...

Embodiment 3

[0049] Embodiment 3: basically the same as Embodiment 1, the difference is:

[0050] The set culture conditions involved in steps 2 to 6 are as follows: light intensity: 2500lx; light time: 13h / d; culture temperature: 22°C.

[0051] The disinfection and sterilization treatment method in step (1) is: soak the cleaned seeds in clean water for 0.7h, after soaking, rinse repeatedly with running water, then rinse twice with distilled water, and then soak in 70% ethanol solution by volume For about 25 seconds, soak in 0.1% mercuric chloride solution for 17 minutes, rinse with sterile water for 5 times, and blot dry on sterile filter paper for later use.

[0052] In step (2), germinate under the set culture conditions for 13 days, and the pH of the germination medium is 6.0.

[0053] The callus induction and differentiation medium in step (3) is: MS+1.5mg / L 6-benzylaminoadenine+0.15mg / L naphthalene acetic acid+mass percentage of 3% sucrose+mass percentage of 0.75% Agar, pH 6.0.

...

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Abstract

The invention discloses an atractylis lancea tissue culturing and rapid propagating method which comprises the following steps: seed processing, disinfecting and sterilizing; building a bacteria-free culturing system; callus inducting, splitting callosity into seedlings; sterile seedling propagating and culturing; sterile seedling root taking and culturing; seedlings acclimatizing and transplanting. According to the method, a large number of the sterile seedlings can be acquired in a short period through the tissue culturing and rapid propagating technology, the problem of atractylis lancea seedlings is solved, the efficient path for large-scale cultivation and production as well as seedling propagation is provided. Through the tissue culturing, the atractylis lancea seedlings with high quality are provided, on this basis, new species with high yield and high quality are bred, novel officinal resources are developed, the imbalance between supply and demand is alleviated and significance for protecting wild atractylis lancea is achieved.

Description

technical field [0001] The invention relates to a method for tissue culture and rapid propagation system, in particular to a method for tissue culture and rapid propagation of Atractylodes atractylodis. Background technique [0002] Cangzhu ( Atractylodes lancea (Thunb.) DC.) is a perennial herbaceous plant of the genus Atractylodes genus in the family Asteraceae, and its rhizome is used as medicine. Mao Atractylodes is pungent, bitter, and warm in nature, and returns to the spleen, stomach, and liver channels. Clinically, it is mainly used for the treatment of abdominal fullness, edema, beriberi, rheumatic arthralgia, wind-cold cold, night blindness and so on. In recent years, the comprehensive development of Atractylodes atractylodes has expanded the use of Atractylodes atractylodes. In addition to medicinal purposes, it can be used as an additive for various beverages and sachets for processing crafts. [0003] Atractylodes atractylodes is mainly distributed in hilly a...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 宋刚徐银曹正杨士虎宋金耀谢正林黄小忠韦颖
Owner JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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