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Jojoba extract useful in improving skin barrier functions

A technology of skin barrier, jojoba, applied in the field of skin care, which can solve the problems of indigestible, non-caloric, etc.

Inactive Publication Date: 2014-07-30
I B R ISRAELI BIOTECH RES LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Like sucrose, jojoba oil is edible, but non-caloric and indigestible

Method used

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  • Jojoba extract useful in improving skin barrier functions
  • Jojoba extract useful in improving skin barrier functions
  • Jojoba extract useful in improving skin barrier functions

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0114] Example 1: Manufacture of jojoba extract

[0115] Vigorous and unpolluted jojoba plants are dark green to brown in color. Therefore, plant parts collected for extraction were checked for their color. Bright yellow leaves or plant parts with spots, especially white spots, were removed. Then, the plant parts are partially or completely dried. The dry plant parts are added to water in a ratio of 1:2 to 1:5, usually 1:2 (plant part: water) at room temperature. The mixture was heated at 100°C for 2 hours and allowed to cool overnight. Solids were removed by centrifugation at 4500 rpm for 30 min (Sigma Laborzentrifugen 3-103645g). The supernatant was collected and filtered through a 1.2 micron filter, then through a 0.45 micron filter and finally through a 0.22 micron filter and the filtrate collected. 1% ascorbic acid 1% and 50% glycerin were added to the filtrate to form the final composition.

[0116] The composition specifications are as follows: dry weight: 36-41...

Embodiment 2

[0117] Example 2: Effect of Jojoba Extract on Gene Expression Profile of Keratinocytes

[0118] With 0.03% Jojoba extract (hBA15m-NHEK batch 15 / 10 / 07, prepared as described in Example 1 above with a leaf:water ratio of 1:2; phenol content 7617.7 mg / L gallic acid equivalent ; Trolox equivalent 108.6 mM; no added preservatives) for cDNA array gene expression studies. Dilutions of source extracts were performed with assay medium (see below).

[0119] Materials and methods

[0120] biological model

[0121] Cell type: normal human epidermal keratinocytes (NHEK) using K at passage 3 074

[0122] Culture conditions: 37°C, 5% CO 2

[0123] Medium: Keratinocyte-SFM (Invitrogen 17005-034) supplemented with epidermal growth factor (EGF) 0.25 ng / ml-pituitary extract (PE) 25 μg / ml (Invitrogen 3700015), gentamicin 25 μg / ml (Sigma G1397) )

[0124] Assay medium: Keratinocyte-SFM (Invitrogen 17005-034) supplemented with gentamicin 25 μg / ml (Sigma G1397)

[0125] Preliminary ...

Embodiment 3

[0185] Example 3: Toxicity Evaluation of Jojoba Extract

[0186] Skin tolerance to jojoba extract was measured by a single application of the extract under an occlusive patch to the dorsal skin of healthy adult volunteers for 48 h.

[0187] Twenty-four volunteers, aged 18-70, female or male, without dermatological lesions in the study area, and able to meet the language requirements of the study participated in this study. Jojoba extract prepared as described in Example 1 above (leaf:water ratio 1:2, phenol content 13, 110 mg / L gallic acid equivalent; Trolox equivalent 92.5 mM) was diluted X2. 0.02ml under the patch was applied to the back skin of each volunteer. Examination of the contact area was performed immediately prior to the study so that the product was applied to a surface free from visible irritation marks, scars, or any abnormalities that might interfere with the reading of the results. Empty patches were applied in parallel as negative controls.

[0188] Afte...

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Abstract

The present invention provides compositions comprising extracts of Jojoba (Simmondsia chinensis) in a polar solvent that are effective in promoting the barrier functions of the skin, useful for improving skin integrity, health and appearance and as anti-aging agent.

Description

field of invention [0001] The present invention relates to the field of skin care, in particular to Jojoba (Simmondsia chinensis) which is effective in promoting the barrier function of the skin, can be used to improve the integrity, structure and strength of the skin and as an anti-aging agent of the skin ) extract. Background of the invention [0002] The skin provides many functions, but its main function is as a protective layer or barrier. The most important role of the skin for terrestrial animals is to protect the water-rich internal organs from a dry external environment. Furthermore, the skin protects the internal tissue from harmful chemical and physical forces and from the invasion of pathogens. [0003] The skin is a structurally complex thick membrane. The skin is composed of the epidermis, dermis, subcutaneous tissue (hypodermis), and adnexal structures (epidermal appendages). The epidermis, the outermost epithelial tissue of the skin, is itself composed of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/92A61K9/06A61Q17/00A61K36/18
CPCA61K8/97A61Q17/00A61Q19/00A61K36/185A61Q19/08A61K8/9789A61P1/16A61P17/00A61P17/02A61P17/04A61P17/06A61P17/16A61P29/00A61P43/00A61K9/0014
Inventor 伊天尼·苏丹特伊拉娜·卡奇科-切尔涅茨基法比安·哈瓦斯奥尔加·本-齐特里特利亚-凡奥本-比撒列伊诺·费里亚历克斯·亚里鲁科
Owner I B R ISRAELI BIOTECH RES LTD
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