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Paper-based microfluidic immunosensor chip and on-site immunoassay platform for timely detection

An immunosensor and microfluidic technology, applied in the field of detection and analysis, can solve the problems of low measurement sensitivity and achieve the effects of short analysis time, favorable fixation and low consumption

Active Publication Date: 2016-01-20
KINGA SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The microfluidic paper chip given in Chinese patent application CN201110270213.2 can only perform qualitative detection
The sensitivity of enzyme-linked immunoassay depends on the sensitivity of the enzyme-catalyzed color reaction. Usually, an antibody can only bind to one molecule of horseradish peroxidase (HRP), and the sensitivity of the assay is low.

Method used

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  • Paper-based microfluidic immunosensor chip and on-site immunoassay platform for timely detection
  • Paper-based microfluidic immunosensor chip and on-site immunoassay platform for timely detection
  • Paper-based microfluidic immunosensor chip and on-site immunoassay platform for timely detection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0095] see figure 1 , the paper-based microfluidic immunosensor chip 10 shown in the present invention prints channel patterns on the paper base with a wax spray printer, puts the paper base into a 90-degree Celsius thermostat and heats it for 5 minutes, so that the surface wax lines penetrate into the paper In the base, a hydrophobic barrier 11 is formed, and the wax-free area in two adjacent wax lines forms a hydrophilic channel 12 for liquid to flow through. The center of the paper-based microfluidic immunosensor chip 10 is provided with a central area 14, The central area 14 radiates to form several hydrophilic channels 12 , and in this embodiment, eight hydrophilic channels 12 are provided. The hydrophilic channel 12 includes a non-closed reaction area 13 for immune reaction.

Embodiment 2

[0097] see figure 2 , the paper-based microfluidic immunosensor chip 20 shown in the present invention prints channel patterns on the paper base with a wax spray printer, puts the paper base into a 90-degree Celsius thermostat and heats it for 5 minutes, so that the surface wax lines penetrate into the paper In the base, several hydrophobic barriers 21 are formed, and the plurality of hydrophobic barriers 21 are arranged in a rectangular array, any one of the hydrophobic barriers 21 is circular, and the area surrounded by any one of the hydrophobic barriers 21 is a closed reaction area for immune reaction 23, the reaction zone 23 is capable of flowing liquid.

[0098] In the above-mentioned embodiment 1 and embodiment 2, in addition to paraffin wax, other hydrophobic polymer materials such as perchlorethylene resin can also be used, and the same effect can also be achieved.

Embodiment 3

[0100] see image 3 , the paper-based microfluidic immune sensor chip 30 shown in the present invention is made by using a small numerically controlled engraving machine after drawing the graphics that need to be engraved with drawing software. The center of the paper-based microfluidic immune sensor chip 30 is provided with a The central area 31 , the central area 31 radiates to form several hydrophilic channels 32 , in this embodiment, there are eight hydrophilic channels 32 . A hydrophobic barrier 33 is provided between any two adjacent hydrophilic channels 32 , and a non-enclosed reaction area 34 for immune reaction is provided adjacent to the central area of ​​the hydrophilic channel 32 .

[0101] The above examples 1-3 are mainly to explain the specific method and structure of the paper-based microfluidic immunosensor chip to construct the microfluidic channel. In addition to the above methods, other methods can also be used, such as inkjet printing, Laser treatment, et...

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Abstract

The invention discloses a paper-based micro-fluidic immunosensor chip and a timely field detection immunoassay platform. According to the paper-based micro-fluidic immunosensor chip, a micro-fluidic channel is constructed on a paper base, and a reaction area in the channel is subjected to surface modification, so that chemical crosslinking is carried out on the channel and an antibody, and the antibody is fixed on the surface of the paper base. The chip washing is needed to be combined with a ring oven, the washing and flow velocity are controlled by the ring temperature, and the problems of the conventional paper-based micro-fluidic chips at home and abroad on washing and flow velocity control are solved. Another cluster marker labeled antibody which is specifically combined with an antigen and takes mesoporous silica or metallic oxide-silica composite nanoparticles as a matrix is detected by adopting manners of mobile phone photographing, image scanning and collecting or laser-induced fluorescence, so that the content of antigen to be detected is detected. According to the paper-based micro-fluidic immunosensor chip, the detection sensitivity is obviously improved.

Description

technical field [0001] The invention belongs to the field of detection and analysis, and in particular relates to a paper-based microfluidic immune sensor chip and an on-site timely detection immune analysis platform. Background technique [0002] Point-of-care-testing (POCT) is a new concept of medical examination in recent years. The detection platform can reduce the number of times patients visit the hospital, reduce the pressure on the hospital, reduce the cost of medical treatment and medical insurance for patients, and increase patient satisfaction. The establishment of POCT is especially important in developing countries, where people living in these countries either lack basic medical resources or cannot afford expensive medical tests. According to the recommendations of the World Health Organization, for developing countries, medical diagnostic equipment should be ASSURED, that is, inexpensive (Affordable), sensitive (Sensitive), special effects (Specific), user-fr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/552B01L3/00
CPCB01L3/502707G01N33/532G01N33/54366G01N33/587
Inventor 章竹君付爱华沈晖林湔章娴君
Owner KINGA SCI & TECH
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