Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Detection and application of a long non-coding RNA

A long-chain non-coding technology for detection reagents, which can be used in the determination/inspection of microorganisms, biochemical equipment and methods, etc., and can solve the problem of less functional research.

Active Publication Date: 2018-05-15
江苏万成生物医学研究院有限公司
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, following the literature, it is found that except for the inventor's research group, there are still few studies on the lncRNA expression profile of esophageal squamous cell carcinoma and the functionality of related indicators.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Detection and application of a long non-coding RNA
  • Detection and application of a long non-coding RNA
  • Detection and application of a long non-coding RNA

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Example 1 lncRNA chip expression analysis of human esophageal squamous cell carcinoma and paired normal tissues

[0050] 1. Materials and methods

[0051] 1. Materials

[0052] Tissue samples were obtained from inpatient surgical resection samples of 6 pairs of patients with esophageal squamous cell carcinoma, each pair containing esophageal tumor tissue and paired normal esophageal tissue.

[0053] 2. Method

[0054] 2.1 Extraction of total RNA from tumor tissue and paired normal tissue

[0055] According to Qiagen's RNA extraction kit (RNeasy Micro Kit, Cat. No. 74004), the total RNA was extracted from the tumor tissues of esophageal phosphocarcinoma patients and the paired normal tissues. The kit contains RNase-Free DNase I (lyophilized), which can effectively remove the impurities of genomic DNA. The purity and concentration of the extracted RNA were quantified by NanoDrop ND-1000 Nucleic Acid Quantifier (NanoDrop Technologies, Wilmington, Delaware), and the qua...

Embodiment 2

[0078] Example 2 Preliminary verification of differential expression of HDESCC-lncRNA4 in cancer tissue and normal tissue of esophageal squamous cell carcinoma by qRT-PCR

[0079] 1. Experimental materials

[0080] Another 30 pairs (different from the samples tested by the microarray) of human esophageal squamous cell carcinoma tissues and paired normal tissues were selected, and the difference in expression of HDESCC-lncRNA4 was initially verified by qRT-PCR.

[0081] 2. Experimental methods and results

[0082] 1 Identification of primer specificity

[0083] 1.1 Screening of specific primers

[0084] (1) Extract the transcript sequence related to HDESCC-lncRNA4 from the Ensemble database, and use the primer design tool (Primer-BLAST) of NCBI to design primers according to the sequence of the transcript;

[0085] (2) The designed primers were evaluated by Oligo7, and 3 pairs of primers were designed for each;

[0086] Pair1 upstream primer: SEQ ID NO.2

[0087] Pair1 dow...

Embodiment 3

[0130] Example 3 qRT-PCR further verified the differential expression of HDESCC-lncRNA4 in cancer tissues and normal tissues of esophageal squamous cell carcinoma

[0131] 1. qRT-PCR kit composition

[0132] 1.1 Composition of dye-based HDESCC-lncRNA4qRT-PCR kit:

[0133] (1) Upstream primer: SEQ ID NO.2

[0134] (2) Downstream primer: SEQ ID NO.3;

[0135] Other reagents refer to SYBR Premix Ex Taq TM II (Tli RNaseH Plus) fluorescence quantitative kit (Code No.RR820A).

[0136] 2. Detection of HDESCC-lncRNA4qRT-PCR

[0137] 2.1 Preparation of total RNA

[0138] Select another 80 pairs of cancer tissues and paired normal tissues of patients with esophageal squamous cell carcinoma, according to the LifeTechnologies company's Reagents (Product No. 15596026) Reagents and steps required to extract total RNA, please refer to the instructions for details. The purity and concentration of the extracted RNA were quantified by NanoDrop ND-1000 Nucleic Acid Quantifier (NanoDrop ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to long non-coding RNA and application thereof. According to the sequence of the long non-coding RNA, specific qRT-PCR (Quantitative Real-time Polymerase Chain Reaction) primers and probes are designed and synthesized and reagents used for esophageal carcinoma auxiliary diagnosis or curative effect prediction are prepared. The qRT-PCR reagents are utilized for detecting the expression level of the long non-coding RNA in specimens of esophageal carcinoma clinical cases and a substantial decrease of the long non-coding RNS expression is found in esophageal carcinoma. The long non-coding RNA can be applied to preparing reagents used for esophageal carcinoma auxiliary diagnosis, curative effect prediction or prognosis judgment.

Description

technical field [0001] The invention belongs to the field of tumor molecular biology, and specifically relates to a long-chain non-coding RNA and its application. Specifically, the present invention relates to the application of a long-chain non-coding RNA in the preparation of preparations for auxiliary diagnosis or prognosis of esophageal cancer. Background technique [0002] Esophageal cancer (Esophageal Carcinoma, EC) is a malignant disease that seriously endangers the health of all human beings. Its morbidity and mortality rank the eighth and sixth respectively in the world. There are two main pathological types of EC: Esophageal Squamous Cell Carcinoma (ESCC) and Esophageal Adenocarcinoma (EAC). In my country, squamous cell carcinoma (90%) is the main pathological type. According to the latest data released by the World Health Organization, there are more than 250,000 new ESCC patients in my country every year, and about 200,000 patients die from esophageal cancer. , f...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6886
CPCC12Q1/6886C12Q2600/158C12Q2600/178
Inventor 曹秀峰李苏卿史卫红仝宇梭庹磊汪春梅谢海伟刘子豪杨同昕吕进纪律朱斌王和明李义生
Owner 江苏万成生物医学研究院有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com