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Preparation method of flat bamboo root antidiabetic polypeptide

An anti-diabetic and Radix radix technology is applied in the field of preparation of anti-diabetic polypeptides from Radix Radix et Rhizoma, which can solve the problems of few researches, no researches yet reported, few researches on hypoglycemic activity, etc., and achieves strong α-glucosidase Inhibitory effect

Inactive Publication Date: 2016-06-22
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Flat bamboo root (Irisjaponica Thunb.) is the rhizome of the iris herb pansies, mainly distributed in East China, South China, Southwest and Hebei, Shaanxi, Gansu and other places. Plants are also traditional Chinese medicinal materials in my country. They are used in traditional medicine for digestion and insecticide, heat-clearing and laxative, and abdominal distension. At present, there are not many studies on the active ingredients of the root of bamboo, and they mainly focus on the research of anti-cancer activity. There are very few studies on its hypoglycemic activity, especially the research on biologically active peptides has not been reported

Method used

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  • Preparation method of flat bamboo root antidiabetic polypeptide
  • Preparation method of flat bamboo root antidiabetic polypeptide
  • Preparation method of flat bamboo root antidiabetic polypeptide

Examples

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Effect test

Embodiment 1

[0022] The preparation method of flat bamboo root antidiabetic polypeptide, the steps are as follows:

[0023] (1) Accurately weigh 300g of bamboo root powder, grind it with liquid nitrogen, add 3L of phosphate buffer and soak for 24 hours, then filter the residue with gauze to obtain the extract of bamboo root, and centrifuge to remove the precipitate. Ammonium sulfate is added to the supernatant to a mass percentage concentration of 40%, overnight, centrifuged again, and the supernatant is removed to obtain a protein extract. Dissolve the protein extract with buffer (the amount of buffer only needs to be enough to completely dissolve the protein extract), put it into a dialysis bag with a molecular weight cut-off range of 6000, dialyze for three days, and then freeze-dry to obtain protein powder.

[0024] (2) Weigh 0.5 g of the protein powder obtained in step (1), make it into a 1% rhizome protein solution, add alkaline protease (purchased from Sigma (P4860)) Alcalase 2.4L t...

Embodiment 2

[0028] The preparation method of flat bamboo root antidiabetic polypeptide, the steps are as follows:

[0029] (1) Accurately weigh 400g of bamboo root powder, grind it with liquid nitrogen, add 1L of phosphate buffer and soak for 24 hours, then filter the residue with gauze to obtain the extract of bamboo root, and centrifuge to remove the precipitate. Ammonium sulfate is added to the supernatant to a mass percentage concentration of 60%, overnight, centrifuged again, and the supernatant is removed to obtain a protein extract. Dissolve the protein extract with buffer (the amount of buffer only needs to be enough to completely dissolve the protein extract), put it into a dialysis bag with a molecular weight cut-off range of 8000, dialyze for three days, and then freeze-dry to obtain protein powder.

[0030] (2) Weigh 1.5 g of the protein powder obtained in step (1), make it into a 2% bamboo root protein solution, add alkaline protease (purchased from Sigma (P4860)) Alcalase 2....

Embodiment 3

[0033] The preparation method of flat bamboo root antidiabetic polypeptide, the steps are as follows:

[0034] (1) Weigh 200g of bamboo root powder, grind it with liquid nitrogen, add 5L of buffer solution and soak for 24 hours, filter the residue with gauze to obtain the extract of bamboo root, and centrifuge to remove the precipitate. Ammonium sulfate is added to the supernatant to a mass percentage concentration of 50%, overnight, centrifuged again, and the supernatant is removed to obtain a protein extract. Dissolve the protein extract with buffer (the amount of buffer only needs to be enough to completely dissolve the protein extract), put it into a dialysis bag with a molecular weight cut-off range of 4000, dialyze for three days, and then freeze-dry to obtain protein powder.

[0035] (2) Weigh 2 g of the protein powder obtained in step (1), make it into a 0.8% concentration of bamboo root protein solution, add alkaline protease (purchased from Sigma (P4860)) Alcalase 2....

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Abstract

The invention provides a preparation method of the anti-diabetic polypeptide of bamboo root. Firstly, leaching with phosphate buffer solution, precipitation with ammonium sulfate, centrifugal dialysis, and separation and purification with Sephadex G-25 column of gel chromatography to obtain the root of bamboo Antidiabetic Peptides. The anti-diabetic polypeptide of the bamboo root obtained in the present invention is mainly a short peptide, the molecular weight ranges from 265 to 700 Da, and has strong α-glucosidase inhibitory activity, with an IC50 of 37.3±3.2 μg / ml. Therefore, the anti-diabetic polypeptide of the bamboo root obtained in the present invention is beneficial to the development and utilization of medicine and other fields.

Description

technical field [0001] The invention relates to a preparation method of an anti-diabetic polypeptide of flat bamboo root, which belongs to the field of plant deep processing and biotechnology. Background technique [0002] Diabetes is a common disease second only to tumors and cardiovascular and cerebrovascular diseases, and more than 95% of them are type 2 diabetes. In the early treatment of type 2 diabetes, controlling postprandial blood glucose levels is the key to treatment; and glucosidase inhibitors have the effect of delaying the absorption of monosaccharides, reducing postprandial hyperglycemia and preventing polysaccharides, so research and development of glucosidase inhibitors It has become a hot spot in diabetes drug research in recent years. The α-glucosidase inhibitors that have been used clinically include doxopin, voglibose, and miglitol, etc., and all of them have achieved good curative effects. [0003] Biologically active peptides are a class of peptide c...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/06C07K1/16A61P3/10
Inventor 张学武戚平白露郑秋平
Owner SOUTH CHINA UNIV OF TECH
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