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Freshwater Chlorella sorokiniana GS30 and application thereof

A technology of chlorella, CCTCCM2013607, applied in the field of biomass energy, can solve the problem of high production cost of microalgae biodiesel, achieve high-concentration carbon dioxide fixation efficiency, biomass yield and oil yield, high-efficiency utilization, and reduce production cost effect

Inactive Publication Date: 2014-07-30
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The purpose of the present invention is to overcome the defects of the prior art, to provide a strain of freshwater chlorella with advantages, which can convert industrial flue gas CO 2 The raw material for biodiesel preparation, this invention can effectively solve the problem of the current treatment of air pollutants from industrial sources while coupling the production of microalgae oil to reduce the high cost of microalgae biodiesel production

Method used

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  • Freshwater Chlorella sorokiniana GS30 and application thereof
  • Freshwater Chlorella sorokiniana GS30 and application thereof
  • Freshwater Chlorella sorokiniana GS30 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Under sterile conditions, single algae were picked from a solid plate and dropped into a 100ml Erlenmeyer flask containing 30ml sterilized BG-11 culture solution, and cultured statically in a light culture rack at a temperature of 28°C and a light intensity of 7500lux for 12 days. Grow to the end of the index and expand the culture with an inoculation ratio of 1:10.

[0051] Prepare 2.4L of BG-11 medium, insert the expanded cultured cells, and inoculate the cells with optical density OD 680 =1.2. The initial pH is 4.0, the temperature is 15°C, the light-to-dark ratio is 8:16, and under the light intensity of 15000lux, 0.5vvm of air (CO 2 The content is 0.03% (v / v)), cultivated for 14 days.

[0052] The algal cells were collected by centrifugation, dried in a vacuum freeze dryer, and the algal powder was weighed and the dry weight was calculated. The biomass concentration of algal powder is 680mg / L, the biomass production rate is 70mg / L / d, CO 2 The fixation efficienc...

Embodiment 2

[0054] Pick a single algae on a solid plate under sterile conditions and drop it into a 100ml Erlenmeyer flask containing 30ml of sterilized culture solution, and place it in a light culture rack for static culture. The temperature is 28°C, and the light intensity is 7500lux. Cultivate for 12 days and grow to an index. At the end of the period, the culture was expanded with a 5% inoculation ratio.

[0055] Prepare 3.1L of BG-11 medium and insert it into the expanded cultured cells. The initial pH is 7.0, the temperature is 45°C, the light-to-dark ratio is 24:0, under the light intensity of 2500lux, 1vvm of 30% CO is introduced 2 (v / v), cultured for 10 days.

[0056] The algal cells were collected by centrifugation, dried in a vacuum freeze dryer, and the dry weight of the algal powder was weighed and calculated. The biomass concentration of algae powder is 2400mg / L, the biomass production rate is 360mg / L / d, CO 2 The fixation efficiency is 660mg / L / d, the oil content determin...

Embodiment 3

[0058] Pick a single algae on a solid plate under sterile conditions and drop it into a 100ml Erlenmeyer flask containing 30ml of sterilized culture solution, and place it in a light culture rack for static culture. The temperature is 28°C, and the light intensity is 7500lux. Cultivate for 12 days and grow to an index. In the final stage, the inoculation ratio was 1:10 to expand the culture.

[0059] Prepare 3.1L of BG-11 medium and insert it into the expanded cultured cells. The initial pH is 11.0, the temperature is 10°C, the light-to-dark ratio is 12:12, and 0.2vvm of 45% CO is introduced under the light intensity of 9500lux. 2 (v / v), cultured for 14 days.

[0060] The algal cells were collected by centrifugation, dried in a vacuum freeze dryer, and the dry weight of the algal powder was weighed and calculated. The biomass concentration of algae powder is 2500mg / L, the biomass production rate is 260mg / L / d, CO 2 The fixation efficiency is 480mg / L / d, the oil content determ...

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Abstract

The invention relates to the technical field of biomass energy, and in particular to a freshwater chlorella strain (i) Chlorella sorokiniana ( / i) GS03 and application thereof to industrial flue gas CO2 fixation and production of microalgae biodiesel. The chlorella strain (i) C.sorokiniana ( / i) GS03 provided by the invention has a preservation number of CCTCC M2013607, and higher CO2 tolerance, higher fixation efficiency of high-concentration carbon dioxide and higher biomass yield and oil yield compared with the reported microalgae strains. Therefore, the invention can greatly reduce microalgal oil production cost, and provides a good production strain for algae for efficient fixation of CO2 in typical industrial flue gas environment and biodiesel production from microalgae oil, and shows great application prospect in the field of biomass energy.

Description

technical field [0001] The invention relates to the technical field of biomass energy, in particular to a freshwater chlorella Chlorella sorokiniana GS03 and its use in industrial flue gas CO 2 Application of immobilization and production of microalgae to biodiesel. Background technique [0002] Due to the extensive use of fossil fuels in the world, the "greenhouse effect" is increasing, and the CO caused by the burning of fossil fuels 2 It accounts for more than 65% of the total "greenhouse effect" gases. Therefore, petrochemical, smelting, electric power and other industries CO 2 Emission reduction has become the focus of sustainable development. [0003] In recent years, some developed countries have used various physical, chemical and biological methods to study CO 2 collection, concentration, fixation and transformation. But compared to traditional physical and chemical CO 2 The sequestration method has the characteristics of high cost, high energy consumption, an...

Claims

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Application Information

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IPC IPC(8): C12N1/12C12P7/64C11B1/00C10L1/02C12R1/89
CPCY02E50/10
Inventor 缪晓玲巩三强
Owner SHANGHAI JIAO TONG UNIV
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