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Method for extraction of heparin sodium and co-production of polypeptide protein powder and amino acid protein powder by utilizing porcine and bovine lungs

A technology of protein powder and heparin sodium, which is applied to the preparation method of peptides, chemical instruments and methods, peptides, etc., can solve the problems of incapable of large-scale production, substandard quality, and low product yield, and achieve short production cycle and high production yield. High efficiency and simple operation

Active Publication Date: 2014-07-30
GUIZHOU HUIJING BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In view of this, the purpose of the present invention is to provide a method for extracting sodium heparin from pig and bovine lungs and co-producing polypeptide protein powder and amino acid protein powder, so as to solve the problem of simple production technology of crude product sodium heparin in my country and low product yield. , the quality is not up to the standard, the benefit is low, and there are deficiencies in the inability to produce on a large scale

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] The method of extracting heparin sodium from pig and bovine lungs and co-producing polypeptide protein powder and amino acid protein powder adopts the following steps:

[0032] (1) pulping: get fresh pig lungs and mince them into a 500um slurry, then add 9% Triton X-100 to the slurry to a weight of 600g, stir intermittently for 35min, press filter to obtain filtrate I and Filter residue Ⅰ;

[0033] (2) Salt solution: first add 1500g of nanofiltration waste salt solution to the filter residue I, control the content of sodium chloride to 4%, then adjust its pH to 9, and control the temperature to 60°C and stir for 5h, press filter to extract Obtain filtrate II and filter residue II;

[0034] (3) Enzymolysis: After mixing the filtrate I and the filtrate II, the filtrate mixed solution is obtained, then add 3 g of trypsin, 2709 alkaline protease and animal protease mixed enzyme in the filtrate mixed solution, and control it at a temperature of 52 ° C. Under the condition ...

Embodiment 2

[0040] The method of extracting heparin sodium from pig and bovine lungs and co-producing polypeptide protein powder and amino acid protein powder adopts the following steps:

[0041] (1) pulping: get the frozen pig lungs and mince them into a 500um slurry, then add 9% Triton X-100 to the slurry with a weight of 400g, stir intermittently for 25min, press filter to obtain the filtrate I and Filter residue Ⅰ;

[0042] (2) Salt solution: first add 500g of nanofiltration waste salt solution to the filter residue I, control the content of sodium chloride to 2%, then adjust its pH to 8, and control the temperature to 40°C and stir for 3h, press filter to extract Obtain filtrate II and filter residue II;

[0043] (3) Enzymolysis: Mix the filtrate I and the filtrate II to obtain a filtrate mixture, then add 1 g of trypsin, 2709 alkaline protease and animal proteolytic enzyme mixture to the filtrate mixture, and control the temperature at 48°C. Under the condition of pH 8.5, the enzy...

Embodiment 3

[0049] The method of extracting heparin sodium from pig and bovine lungs and co-producing polypeptide protein powder and amino acid protein powder adopts the following steps:

[0050] (1) Pulping: get fresh bovine lung and mince it into a 500um slurry, then add 9% Triton X-100 to the slurry with a weight of 500g, stir intermittently for 30min, press filter to obtain filtrate I and Filter residue Ⅰ;

[0051] (2) Salt solution: first add 1000g of nanofiltration waste salt solution to the filter residue I, control the content of sodium chloride to 3%, then adjust its pH to 8.5, and control the temperature to 50°C and stir for 4 hours, press filter to extract Obtain filtrate II and filter residue II;

[0052] (3) Enzymolysis: Mix the filtrate I and the filtrate II to obtain the filtrate mixed solution, then add 2 g of trypsin, 2709 alkaline protease and animal protease mixed enzyme to the filtrate mixed solution, and control it at a temperature of 50 ° C. Under the condition of ...

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Abstract

The invention discloses a method for extraction of heparin sodium and co-production of polypeptide protein powder and amino acid protein powder by utilizing porcine and bovine lungs, belonging to the technical field of manufacturing of biomedicines. The method adopts the steps of (1) pulping; (2) salt solution; (3) enzymolysis; (4) precipitation and adsorption; (5) elution and drying; (6) preparation of the polypeptide protein powder; (7) preparation of the amino acid protein powder. The method disclosed by the invention has the advantages of reasonable process design, no emission of waste gas, waste water and waste residue and no environmental pollution and is suitable for large-scale production; waste liquid and the waste residue produced in the production process are recycled and turned into treasure, so that economic benefits are improved; the operation is simple, the production period is short, the production yield is high and the method is suitable for popularization and application.

Description

technical field [0001] The invention relates to the technical field of biomedicine production, in particular to a method for extracting heparin sodium from pig and bovine lungs and co-producing polypeptide protein powder and amino acid protein powder. Background technique [0002] Heparin was discovered in the liver of dogs in 1916 and is widely found in the liver, lungs, intestines and other organs of animals. Heparin has the function of preventing blood coagulation and dissolving thrombus, and is an excellent natural anticoagulant drug. At present, heparin is the most effective and clinically used anticoagulant drug in the world. European and American countries were the first to extract a large amount of heparin from bovine lung and bovine intestine , the extraction of heparin was stopped due to the prevalence of mad cow disease. Later, relevant experts found a large amount of heparin in the small intestine of pigs, and because my country is a big pig-raising country, the r...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/10C12P21/06C07K1/36C07K1/34C07K1/30C12P13/04
Inventor 李能强
Owner GUIZHOU HUIJING BIOTECH
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