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Preparation and application of sandwich-type multilayer immobilized bioactive filler

A biologically active, sandwich-type technology, applied in the field of water treatment, can solve the problems of weakened mechanical strength, easy falling off of embedded bodies, shortened service life of fillers, etc., to reduce the formation of dense films, high substrate mass transfer efficiency, and effective The effect of large specific surface area

Active Publication Date: 2014-07-30
湖南汨罗循环经济产业园区科技创新服务中心
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  • Abstract
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Problems solved by technology

This method is simple to operate, but has great limitations. The prepared microspheres and embedded blocks are prone to water-soluble swelling. During use, the mechanical strength of the microspheres and embedded blocks will be greatly weakened due to water-soluble swelling, and even broken. Phenomenon, thus shortening the service life of the filler, which cannot be ideally applied to engineering practice
Experimental studies have shown that the fillers prepared by combining traditional carriers such as Raschig rings, Pall rings, stepped rings, and suspended modified fillers with embeddings through adhesion have poor overall stability and embeddings are easy to Problems such as shedding and easy loss of bacteria

Method used

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  • Preparation and application of sandwich-type multilayer immobilized bioactive filler
  • Preparation and application of sandwich-type multilayer immobilized bioactive filler
  • Preparation and application of sandwich-type multilayer immobilized bioactive filler

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Embodiment Construction

[0020] The present invention is now further described by taking nitrifying bacteria as an example. This embodiment is intended to further illustrate the immobilization, preparation and use of biologically active fillers for nitrifying bacteria, but the embodiments of the present invention are not limited thereto.

[0021] (1) Centrifuge and concentrate the domesticated nitrifying bacteria suspension to obtain a concentrated solution of nitrifying bacteria, take 45L of centrifuged and the bacteria content is 7.5x10 8 The concentrated solution of nitrifying bacteria per mL is ready for use;

[0022] (2) Take 30.0kg of polyvinyl alcohol in a water bath and heat it to 90°C and dissolve it in 200L of water to obtain a polyvinyl alcohol solution;

[0023] (3) Cool the polyvinyl alcohol solution in step (2) to 30°C and fully mix it with the bacterial concentrate obtained in step (1), add water to make the volume to 300L to obtain an embedding solution, and the polyvinyl alcohol in th...

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Abstract

The invention discloses preparation and an application of a sandwich-type multilayer immobilized bioactive filler, and belongs to the technical field of water treatment. The bioactive filler is composed of two parts: embedding bodies and non-woven carriers; the embedding bodies are obtained by boric acid secondary cross-linking curing of an embedding liquid; the embedding bodies and the non-woven carriers are in alternative arrangement; fiber yarns of the non-woven carriers and the embedding bodies are combined into a stable organic whole; the embedding liquid formed by mixing a bacteria concentrated solution and a polyvinyl alcohol solution evenly coats the non-woven carriers, multiple layers of carriers are overlaid together, a layered filler having a sandwich-like structure is formed after boric acid secondary cross-linking curing, and then the granular bioactive filler is prepared through cutting the layered filler. The problems that bacteria in a traditional biochemical method are low in concentration, easy to lose and the like are solved, and moreover, the sandwich-like layered structure formed by the non-woven fabrics and the embedding bodies improves the stability of the bioactive filler, and the bioactive filler has a good application prospect.

Description

technical field [0001] The invention relates to the preparation and application of a sandwich structure immobilized bioactive filler in water treatment, belonging to the field of water treatment. Background technique [0002] Microbial cell immobilization technology can greatly increase the concentration of microorganisms, make it difficult for microorganisms to lose, shorten the start-up time of the reactor, purify and create the ecological advantages of characteristic bacteria, and the immobilized bioactive filler has the ability of anti-toxicity, acid and alkali resistance, and salt resistance It is obviously enhanced and has a good application prospect. The commonly used immobilization methods of microbial cells include adsorption, cross-linking and embedding. Among them, the embedding method is the most commonly used, and the materials that have been successfully used for microbial cell embedding include: polyvinyl alcohol, agar, K-carrageenan, gelatin, sodium alginate...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F3/10C02F3/34C12N11/08C12N11/04
CPCY02W10/10
Inventor 杨宏管清坤尚海源吴城锋王猛陈伟鄢琳王小乐孟婷陶慕翔胡希佳赵月兰王玉洁姚仁达
Owner 湖南汨罗循环经济产业园区科技创新服务中心
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