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A preparation method of bone graft material coated with adiponectin

A technology of adiponectin and bone grafting, applied in the field of medical biomaterial preparation, can solve the problems of refractory degradation, poor biological activity, poor bone powder strength, etc., and achieve the effects of easy degradation, high biological activity, and increased bone strength

Active Publication Date: 2015-08-05
PEKING UNIV SCHOOL OF STOMATOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

To solve the shortcomings of the existing bone meal such as poor strength, difficult to degrade, and poor biological activity

Method used

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  • A preparation method of bone graft material coated with adiponectin
  • A preparation method of bone graft material coated with adiponectin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] The preparation method of the bone graft material coated with adiponectin comprises the following steps:

[0030] Step 1, prepare modified calcined bone material; select the joint head of newborn calf as the bone raw material, fully wash with deionized water and basically remove collagen by physical or biochemical methods; then use diammonium hydrogen phosphate to balance according to the chemical reaction Modify the bone matrix (bone salt) with the formula ratio, so that the Ca / P atomic ratio of the bone matrix is ​​reduced to 1.55; then the modified bone material is calcined at a temperature of 1000°C to completely remove the immune antigenicity of the bone material , to obtain a modified calcined bone material; in the obtained modified calcined bone material, hydroxyapatite (HA):β tricalcium phosphate (βTCP)=1:3.

[0031] Step 2, take 2500mg molecular weight at 2.5x10 5 Carboxymethyl chitosan, the carboxymethyl chitosan is dissolved in 60ml volume fraction of 2% ace...

Embodiment 2

[0036] The preparation method of the bone graft material coated with adiponectin comprises the following steps:

[0037] Step 1, prepare modified calcined bone material; select the epiphysis of the tibia as the bone material, fully wash with deionized water and basically remove collagen by physical or biochemical methods; then use ammonium dihydrogen phosphate to balance according to the chemical reaction Modification of the bone matrix (bone salt) by formula matching, so that the Ca / P atomic ratio of the bone matrix is ​​reduced to 1.5; then the modified bone material is calcined at a temperature of 800°C to completely remove the immunoantigenicity of the bone material , to obtain modified calcined bone material.

[0038] Step 2, take 2000mg molecular weight in 2x10 5 Carboxymethyl chitosan, said carboxymethyl chitosan is dissolved in 70ml volume fraction is 2% acetic acid solution to form carboxymethyl chitosan solution; Said solution is mixed with 1000mg modified calcined ...

Embodiment 3

[0043] The preparation method of the bone graft material coated with adiponectin comprises the following steps:

[0044] Step 1, prepare modified calcined bone material; select the joint head of newborn calf as the bone material, thoroughly wash with deionized water and basically remove collagen by physical or biochemical methods; then use phosphoric acid to formulate according to the chemical reaction balance equation Modified the bone matrix (bone salt) to reduce the Ca / P atomic ratio of the bone matrix to 1.66; then calcined the modified bone material at a temperature of 1100°C to completely remove the immunoantigenicity of the bone material, and obtained Modification of calcined bone material.

[0045] Step 2, take 3000mg molecular weight in 3x10 5 Carboxymethyl chitosan, the carboxymethyl chitosan is dissolved in 80ml volume fraction of 2% acetic acid solution to form a carboxymethyl chitosan solution; the solution is mixed with 1000mg modified calcined bone material to ...

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Abstract

The invention relates to a preparation method of a bone grafting material coated with adiponectin. The preparation method comprises the following steps: step 1, preparing a modified calcined bone material; step 2, taking 2-3 parts by weight of carboxymethyl chitosan having the molecular weight of 2*10<5> to 3*10<5>, and dissolving the carboxymethyl chitosan in an acidic solution to form a carboxymethyl chitosan solution; and mixing the solution with 1 part by weight of the modified calcined bone material to form a mixture; and step 3, carrying out Co60 irradiation sterilization of the mixture of the step 2, then adding human recombinant adiponectin into the mixture, uniformly mixing, molding, carrying out freeze drying, and thus obtaining the bone grafting material coated with adiponectin. The bone grafting material coated with adiponectin and prepared by the method is large in bone strength, easy to degrade and large in biological activity, and besides has a bone induction characteristic.

Description

technical field [0001] The invention relates to the technical field of preparation of medical biological materials, in particular to a preparation method of a high-activity adiponectin-coated bone graft material loaded with biological factors and sustained release. The prepared material can be applied to the bone defect of the body repair. Background technique [0002] There are many methods for bone defect repair and reconstruction in the prior art, but repair and reconstruction after bone defect, especially repair and reconstruction after large bone defect has always been a difficult problem for orthopedics. Seeking ideal substitute materials for bone repair has always been a key issue to be solved in orthopedics and related disciplines. [0003] Years of research results have shown that among the members of the apatite ceramic family, the crystal structure of nano-hydroxyapatite (HA) is basically the same as the structure of mineral particles in bone tissue, and calcium ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K38/17A61L27/34A61L27/58A61L27/54
Inventor 唐志辉毋育伟李箐
Owner PEKING UNIV SCHOOL OF STOMATOLOGY
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