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Preparation method of natural marine compound polypeptide moisturizing agent

A technology of moisturizing agent and compound enzyme preparation, which is applied in skin care preparations, pharmaceutical formulations, cosmetic preparations, etc., can solve the problem that hydrolase cannot be recycled, achieve a wide range of products, improve transdermal absorption effect, no The effect of three industrial wastes

Active Publication Date: 2014-07-30
SOUTH CHINA UNIV OF TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, the relevant research on enzymatic hydrolysis of marine protein is mainly used in food and health products, and the hydrolytic enzymes used cannot be recycled, and there are few reports on the industrial scale production of marine compound polypeptide moisturizers through enzymatic modification

Method used

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  • Preparation method of natural marine compound polypeptide moisturizing agent
  • Preparation method of natural marine compound polypeptide moisturizing agent
  • Preparation method of natural marine compound polypeptide moisturizing agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Preparation of marine animal and plant protein powder: wash, pulverize and enzymolyze the crab scraps (the compound enzyme preparation is alkaline protease and neutral protease, the ratio by weight is 1:0.5. The addition amount of the compound enzyme preparation is 1.5%, hydrolysis The temperature is 40°C, the pH is 6.5, the hydrolysis time is 4h), the centrifugal speed is 3000rpm, the pH is adjusted to 5, filtered, decolorized, and finally spray-dried.

[0027] Weigh 100kg of marine animal and plant protein powder, mix it with 2500kg of 50°C warm water in a reaction kettle, adjust the pH to 6.5, and then add compound protease preparations (alkaline protease, neutral protease, papain, flavor protease (all purchased from Novi Xin (China) Biotechnology Co., Ltd.) with a mass ratio of 1:0.3:0.2:0.2), so that its mass percentage concentration in the solution is 1.5%, hydrolyzed at a temperature of 40 ° C for 4 hours, and then the fixed Decolorization, filtration, and decolo...

Embodiment 2

[0035] Preparation of marine animal and plant protein powder: wash, crush and enzymatically hydrolyze the leftovers of crab and fish (mixed according to the mass fraction of 1:1) (the compound enzyme preparation is alkaline protease and neutral protease, and the weight ratio is 1:1 The addition of compound enzyme preparation is 1.5%, the hydrolysis temperature is 40°C, the pH is 6.5, and the hydrolysis time is 4h), centrifuged (speed 4000rpm), adjusted to pH 6, filtered, decolorized, and finally spray-dried.

[0036]Weigh 100kg of marine animal and plant protein powder, mix it with 3000kg of 40°C warm water in a reaction kettle, adjust the pH to 8.0, and then add compound protease preparations (alkaline protease, neutral protease, papain, flavor protease (all purchased from Novi Xin (China) Biotechnology Co., Ltd.) with a mass ratio of 1:0.5:0.1:0.1), so that its mass percentage concentration in the solution is 0.5%, hydrolyzed at a temperature of 40°C for 6 hours, and then the...

Embodiment 3

[0044] Preparation of marine animal and plant protein powder: wash, pulverize and enzymatically hydrolyze crab scraps (the compound enzyme preparation is alkaline protease and neutral protease, the ratio by weight is 1:0.8. The addition of compound enzyme preparation is 1.5%, hydrolysis Temperature 40°C, pH 6.5, hydrolysis time 4h), centrifugation (speed 5000rpm), adjust pH to 7, filter, decolorize, and finally spray dry.

[0045] Weigh 100kg of marine animal and plant protein powder, mix it with 4200kg of warm water at 50°C in a reaction kettle, adjust the pH to 7.0, and then add compound protease preparations (alkaline protease, neutral protease, papain, flavor protease (all purchased from Nuo Weixin (China) Biotechnology Co., Ltd.) was compounded at a mass ratio of 1:0.3:0.2:0.2), so that its mass percentage concentration in the solution was 0.5%, hydrolyzed at a temperature of 50°C for 8 hours, and then separated Immobilized enzyme, filtration, decolorization (activated ca...

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Abstract

The invention discloses a preparation method of a natural marine compound polypeptide moisturizing agent, which can be used as a functional additive for daily chemicals. An enzyme immobilization method is employed; a marine animal and plant protein powder and water in a weight ratio of 1:5-1:50 are added with a compound enzyme prepared through an immobilized enzyme technology, wherein the mass concentration of compound enzyme in the solution is 0.1-5%, and a reaction is carried out at the temperature of 30-80 DEG C, under pH value of 5-10 for a hydrolysis time of 1-13 h; and then the immobilized enzyme is separated out, and the reaction products are filtered, decolored and desalinized to obtain the marine compound polypeptide moisturizing agent. Compared with a traditional method, the method has the advantages of multitime recycling of the immobilized compound enzyme, mild hydrolysis conditions, high hydrolysis efficiency and no generation of industrial wastes, and is in line with the new trend of requirements of naturalness, environment-friendliness, safety and non-chemical resource on functional additives for daily chemicals.

Description

technical field [0001] The invention relates to the field of comprehensive development and application of marine proteins, in particular to a preparation method of a natural marine composite polypeptide moisturizer, which belongs to the technical field of production of daily chemical additives. Background technique [0002] About 400,000 species of organisms live in the ocean, which accounts for 71% of the earth's area, accounting for 80% of the total number of species on the earth. Marine organisms growing in the special environment of the ocean (high salt, high pressure, lack of oxygen, lack of sunlight, etc.) produce and accumulate a large number of substances with special chemical structures and special physiological activities and functions during their growth and metabolism. Substances are important resources for the development of new marine moisturizers. The research and development of marine moisturizing agent is of great significance to make full use of abundant m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/64A61Q19/00
Inventor 程建华王丽杰杨敏
Owner SOUTH CHINA UNIV OF TECH
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