Preparation method of glucose precursor for improving dairy cow rumen flora
A technology of rumen flora and glucose, which is applied in the field of glucose precursors in dairy cows, can solve the problems of untargeted supplementation, palliative treatment but not the root cause, and poor treatment effect of primary ketosis
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Embodiment 1
[0101] The preparation of embodiment 1 microbial preparation
[0102] The preparation method of described microbial preparation comprises the steps:
[0103] (1) Activation of Aspergillus niger CGMCC NO.7927 and Clostridium rumenus CGMCC NO.2125
[0104] Inoculate the well-preserved slant strain of Aspergillus niger CGMCC NO.7927 on the slant medium, culture at 30°C for 24 hours to activate the strain, and activate it 3 times;
[0105] Inoculate the well-preserved slant strain of Clostridium rumenensis CGMCC NO.2125 on the slant medium, culture at 39°C for 24-36h to activate the strain, and activate it twice;
[0106] The Aspergillus niger CGMCC NO.7927 slant medium consists of: 200g of potatoes (boiled juice), 20g of glucose, 20g of agar, 10g of Chinese herbal medicine extract, 1000mL of distilled water, pH value 5.8, sterilized at 121°C for 20min;
[0107] The slant medium of Clostridium rumenum CGMCC NO.2125 consists of: beef extract 3g, sodium chloride 5g, peptone 10g, a...
Embodiment 2
[0141] The preparation of embodiment 2 Chinese herbal medicine extract
[0142] The Chinese herbal medicine extract is prepared from the following raw materials in parts by weight: 60 parts of angelica, 60 parts of Chuanxiong, 55 parts of Amomum, 55 parts of Radix Paeoniae Rubra, 55 parts of Rehmannia glutinosa, 50 parts of Divine Comedy, 50 parts of malt, 50 parts of motherwort, Guangmu 42 parts of incense, 42 parts of codonopsis, 42 parts of herb, 42 parts of astragalus, 42 parts of honeysuckle, 40 parts of Houttuynia cordata, 40 parts of Poria cocos, 40 parts of jujube kernel, 40 parts of Fushen, 35 parts of polygala, 35 parts of fried fennel, Atractylodes macrocephala 35 parts, Magnolia officinalis 35 parts, Citrus aurantium 30 parts, Licorice 30 parts, Hawthorn 30 parts, Chenpi 30 parts.
[0143]The preparation method of the Chinese herbal medicine extract is as follows: respectively pulverize the above-mentioned Chinese herbal medicines to a particle size of less than 2 ...
Embodiment 3
[0146] The composite of embodiment 3 complex enzymes
[0147] The compound enzyme is formed by uniformly mixing the following solid enzyme preparations in parts by weight: 25 parts of neutral protease, 25 parts of acid protease, 25 parts of alkaline xylanase, 25 parts of acid xylanase, 25 parts of cellulase 18 parts of β-glucanase, 18 parts of pectinase, 18 parts of amylase, 12 parts of mannase, and 12 parts of phytase.
[0148] Using the microbial preparations prepared in Examples 1-3, Chinese herbal medicine extracts and compound enzymes as raw materials, and commercially available propylene glycol, glycerin, calcium propionate, aluminosilicate, nicotinamide, nicotinic acid, sodium lactate, sodium chloride, lemon Acid, rumen-passed glucose, rumen-passed amino acid, rumen-passed choline, multivitamins, and compound minerals are compounded to prepare glucose precursors that can improve the rumen flora of dairy cows. The specific raw material formula is shown in Table 1, and th...
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