A method for rapid detection of antioxidant components in rhubarb seeds
An anti-oxidant ingredient, the technology of rhubarb, applied in the direction of measuring devices, material separation, and analysis of materials, can solve the problems of inability to compare the antioxidant activity and the inability to know the antioxidant active ingredients, etc., to achieve easy promotion, short detection time, and sensitivity and highly specific effects
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Embodiment 1
[0020] Embodiment 1 A method for rapidly detecting antioxidant components in rhubarb seeds, comprising the following steps:
[0021] (1) After crushing rhubarb seeds to 40 mesh, rhubarb seed powder is obtained.
[0022] (2) The rhubarb seed powder was ultrasonically extracted with 50 times its mass of methanol with a volume concentration of 50% under the condition of a power of 200W for 50 minutes, and the rhubarb seed extract was obtained after filtration.
[0023] (3) The rhubarb seed extract is separated by a high-performance liquid chromatography column, and the separated components first flow through the first ultraviolet detector as a sample. The mass concentration of the sample and the second pump as the mobile phase is 10 mg / After the antioxidant 1,1-diphenyl-2-trinitrophenylhydrazine of L is derivatized in the reaction loop, it enters the second UV detector, and then the first UV detector and the second UV detector The recorded chromatograms are compared, and when a...
Embodiment 2
[0028] Embodiment 2 A method for rapidly detecting antioxidant components in rhubarb seeds, comprising the following steps:
[0029] (1) After crushing rhubarb seeds to 60 mesh, rhubarb seed powder is obtained.
[0030] (2) The rhubarb seed powder was ultrasonically extracted for 30 min with 100 times its mass of methanol with a volume concentration of 80% under the condition of a power of 300 W, and the rhubarb seed extract was obtained after filtration.
[0031] (3) The rhubarb seed extract is separated by a high-performance liquid chromatography column, and the separated components first flow through the first ultraviolet detector as a sample. The mass concentration of the sample and the second pump as the mobile phase is 100 mg / After the antioxidant 1,1-diphenyl-2-trinitrophenylhydrazine of L is derivatized in the reaction loop, it enters the second UV detector, and then the first UV detector and the second UV detector The recorded chromatograms are compared, and when an...
Embodiment 3
[0036] Embodiment 3 A method for rapidly detecting antioxidant components in rhubarb seeds, comprising the following steps:
[0037] (1) After crushing rhubarb seeds to 40 mesh, rhubarb seed powder is obtained.
[0038] (2) The rhubarb seed powder was ultrasonically extracted with 100 times its mass of 100% methanol at a power of 300W for 30 minutes, and filtered to obtain rhubarb seed extract.
[0039] (3) The rhubarb seed extract is separated by a high-performance liquid chromatography column, and the separated components first flow through the first ultraviolet detector as a sample. The mass concentration of the sample and the second pump as the mobile phase is 200 mg / After the antioxidant 1,1-diphenyl-2-trinitrophenylhydrazine of L is derivatized in the reaction loop, it enters the second UV detector, and then the first UV detector and the second UV detector The recorded chromatograms are compared, and when an inverted peak compound appears in the chromatogram recorded b...
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