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Method for rapidly detecting antioxidant ingredients in rhubarb seeds

An anti-oxidant ingredient, the technology of rhubarb, applied in the direction of measuring devices, material separation, and analysis of materials, can solve the problems of inability to compare the antioxidant activity and the inability to know the antioxidant active ingredients, etc., to achieve easy promotion, short detection time, and sensitivity and highly specific effects

Active Publication Date: 2014-07-23
BEIJING TRT HEALTH PHARMA QINGHAI CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the DPPH method can only analyze the total antioxidant activity in the extract, and cannot know the antioxidant active ingredients, and cannot compare the antioxidant activity of each active substance

Method used

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  • Method for rapidly detecting antioxidant ingredients in rhubarb seeds

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1 A method for rapidly detecting antioxidant components in rhubarb seeds, including the following steps:

[0021] ⑴ After crushing rhubarb seeds to 40 meshes, the rhubarb seed powder is obtained.

[0022] ⑵The rhubarb seed powder was ultrasonically extracted with 50 times its mass of methanol with a volume concentration of 50% at a power of 200W for 50 minutes, and the rhubarb seed extract was obtained after filtration.

[0023] ⑶ The rhubarb seed extract is separated by a high performance liquid chromatography column, and the separated components are first passed through the first UV detector and then used as a sample. The sample and the second pump are pumped as mobile phase with a mass concentration of 10 mg / After L’s antioxidant 1,1-diphenyl-2 trinitrophenylhydrazine is derivatized in the reaction ring, it enters the second ultraviolet detector, and then the first ultraviolet detector and the second ultraviolet detector are The recorded chromatograms are com...

Embodiment 2

[0028] Example 2 A method for rapidly detecting antioxidant components in rhubarb seeds, including the following steps:

[0029] (1) After crushing the rhubarb seeds to 60 meshes, the rhubarb seed powder is obtained.

[0030] (2) The rhubarb seed powder was ultrasonically extracted with 80% methanol with a volume concentration of 100 times its mass under the condition of 300W for 30 minutes, and the rhubarb seed extract was obtained after filtration.

[0031] ⑶ The rhubarb seed extract is separated by a high performance liquid chromatography column, and the separated components first flow through the first UV detector and then used as a sample. The mass concentration of the sample and the second pump as the mobile phase pumped is 100 mg / After L’s antioxidant 1,1-diphenyl-2 trinitrophenylhydrazine is derivatized in the reaction ring, it enters the second ultraviolet detector, and then the first ultraviolet detector and the second ultraviolet detector are The recorded chromatogr...

Embodiment 3

[0036] Example 3 A method for rapidly detecting antioxidant components in rhubarb seeds, including the following steps:

[0037] ⑴ After crushing rhubarb seeds to 40 meshes, the rhubarb seed powder is obtained.

[0038] (2) The rhubarb seed powder was ultrasonically extracted with methanol with a volume concentration of 100%, which was 100 times its mass, under the condition of a power of 300W for 30 minutes, and the rhubarb seed extract was obtained after filtration.

[0039] ⑶ The rhubarb seed extract is separated by a high performance liquid chromatography column, and the separated components are first passed through the first UV detector and then used as a sample. The mass concentration of the sample and the second pump as the mobile phase pumped is 200 mg / After L’s antioxidant 1,1-diphenyl-2 trinitrophenylhydrazine is derivatized in the reaction ring, it enters the second ultraviolet detector, and then the first ultraviolet detector and the second ultraviolet detector are ...

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Abstract

The invention relates to a method for rapidly detecting antioxidant ingredients in rhubarb seeds. The method comprises the following steps: (1) crushing the rhubarb seeds to obtain rhubarb seed powder; (2) extracting the rhubarb seed powder by using methanol, and filtering to obtain a rhubarb seed extracting solution; (2) performing chromatographic column separation on the rhubarb seed extracting solution by using a high performance liquid chromatography, enabling the separated ingredients to flow through a first ultraviolet detector to serve as a sample, deriving the sample and an antioxidant, namely 1,1-biphenyl-2trinitrobenzene hydrazine which is pumped, serves as a mobile phase in a second pump and has the mass concentration of 10-200mg / L in a reactive loop, enabling the product to enter a second ultraviolet detector, and comparing chromatogram maps recorded by the first ultraviolet detector and the second ultraviolet detector, wherein when a compound with a negative peak occurs in the chromatogram map recorded by the second ultraviolet detector, the compound is the antioxidant ingredient in the extract. The method is simple and rapid, and the antioxidant ingredients in rhubarb seeds can be effectively evaluated.

Description

technical field [0001] The invention relates to a method for rapidly detecting antioxidant components, in particular to a method for rapidly detecting antioxidant components in rhubarb seeds. Background technique [0002] The traditional Chinese medicine Rhubarb is the dried root and rhizome of Rheum tanguticum Maxim. ex Balf . , Rheum palmatum Linn. , or Rh. of f icinale Baill. Tangut rhubarb, also known as Junmuzha (Tibetan name), or rhubarb with chicken feet, belongs to the authentic medicinal materials in Qinghai Province. There are also distributions in the northwest of Sichuan. Rhubarb palmatum is mainly produced in Gansu, Qinghai, Tibet, Sichuan and other places. It is mainly cultivated and has the largest yield. Medicinal rhubarb is mainly produced in Sichuan, Guizhou, Yunnan, Hubei, Shaanxi and other places, cultivated or wild. [0003] The seeds of medicinal materials are the source of production and development of medicinal materials, the internal factors that ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
Inventor 董琦谭亮胡风祖肖远灿迟晓峰耿丹丹
Owner BEIJING TRT HEALTH PHARMA QINGHAI CO LTD
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