High efficient composite inoculant for producing ethanol from straws, preparation and application thereof

A technology of compound bacterial agent and straw, which is applied in the field of environmental protection, can solve the problems of low degradation efficiency, large environmental impact, and high operation cost, and achieve the effects of simple equipment, improved utilization efficiency, and improved efficiency

Inactive Publication Date: 2014-07-23
NANKAI UNIV +1
View PDF6 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the method of using straw to produce ethanol has high requirements on equipment, high energy consumption, high operating cost and great impact on the environment; in contrast, the biological treatment method consumes less energy and is environmentally friendly, but there are The disadvantage of low degradation efficiency

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] A high-efficiency composite bacterial agent for ethanol production from straw, which is composed of No. I bacterial agent and No. II bacterial agent. The mass ratio is 1:1:1; No. II bacterial agent is composed of Saccharomyces cerevisiae and Hansenula yeast, and the mass ratio of Saccharomyces cerevisiae and Hansenula yeast is 2:1; The mass percentage of No. I bacterial agent in the composite bacterial agent is 50%.

[0024] The preparation method of the high-efficiency composite microbial agent for producing ethanol from straws has the following steps:

[0025] 1) After the selected strains were inoculated in the corresponding culture medium, shake culture at 170 rpm in a shaker at 40°C for 36 h, and then diluted into each individual strain;

[0026] 2) Mix the above-mentioned single-strain bacterial liquids according to the mass ratio of No. I bacterial agent and No. II bacterial agent in the composite bacterial agent, each of which is 50%, to prepare a composite bac...

Embodiment 2

[0036] A high-efficiency composite bacterial agent for ethanol production from straw, which is composed of No. I bacterial agent and No. II bacterial agent. The mass ratio of bacteria is 1:1:2; No. II bacterial agent is composed of Saccharomyces cerevisiae and Candida, and the mass ratio of Saccharomyces cerevisiae and Candida is 2:1; the mass of No. I bacterial agent in the composite bacterial agent The percentage is 60%.

[0037] The preparation method of the high-efficiency composite microbial agent for producing ethanol from straws has the following steps:

[0038] 1) After the selected strains were inoculated in the corresponding culture medium, shake culture at 170 rpm in a shaker at 40°C for 72 hours, and then diluted into each individual strain;

[0039] 2) The above-mentioned single-strain bacterial liquids were mixed according to the mass ratio of No. I bacterial agent and No. II bacterial agent in the composite bacterial agent to be 60% and 40% respectively, to pre...

Embodiment 3

[0049] A high-efficiency composite bacterial agent for producing ethanol from straw, which is composed of No. I bacterial agent and No. II bacterial agent. The mass ratio of bacteria is 1:1:2; No. II bacterial agent is composed of Hansenula and Candida, and the mass ratio of Hansenula and Candida is 1:1; the mass of No. I bacterial agent in the composite bacterial agent The percentage is 60%.

[0050] The preparation method of the high-efficiency composite microbial agent for producing ethanol from straws has the following steps:

[0051] 1) After the selected strains were inoculated in the corresponding culture medium, shake culture at 170 rpm in a shaker at 40°C for 50 hours, and then diluted into each individual strain;

[0052] 2) Mix the above-mentioned single-strain bacterial liquid according to the mass ratio of No. I and No. II bacterial agents in the composite bacterial agent to be 70% and 30% respectively, to prepare a composite bacterial agent.

[0053] Should The...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
particle diameteraaaaaaaaaa
particle diameteraaaaaaaaaa
particle diameteraaaaaaaaaa
Login to view more

Abstract

The invention discloses a high efficient composite inoculant for producing ethanol from straws. The inoculant is composed of an inoculant I and an inoculant II; wherein the inoculant I is composed of one or more components selected from trichoderma, blue mould, aspergillus, and actinomycetes according to any ratio, the inoculant II is composed of one or more components selected from beer yeast, hansenula, and candida according to any ratio, and the inoculant I accounts for 50 to 70% of the total weight of the composite inoculant. The composite inoculant can be used to ethanol production through straw fermentation. The invention has the advantages that: (1) the straw powder is processed by being soaked in imidazole acetate so as to improve the cellulose dissolving performance, and thus the cellulose is easier to absorb; (2) the composite inoculant is used to co-ferment the straw to produce ethanol, during the degradation process, different microorganisms secrete complementary enzymes to completely degrade the substrate through metabolism, and thus the fermentation efficiency is improved; (3) the equipment and the technology is simple, the technology is environment-friendly, and the biomass resource utilization rate is improved.

Description

technical field [0001] The invention relates to the technical field of environmental protection, in particular to a high-efficiency composite bacterial agent for ethanol production from straw and an application method thereof. Background technique [0002] With the gradual depletion of disposable fossil fuel resources and the increasing tension in energy and resources, how to efficiently and comprehensively utilize and digest renewable energy—biomass energy has become a topic of widespread concern around the world. Microbial fermentation to produce fuel ethanol as a renewable energy has attracted people's attention. At present, large-scale production of fuel ethanol at home and abroad is based on sugary and starchy crops such as sugar cane, molasses, cassava, and corn. In my country, corn, aged wheat and rice are mainly used as raw materials, which is undoubtedly a heavy burden for my country's relatively scarce food. Cellulosic biomass resources are very rich, but most of...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12N1/20C12P7/08C12R1/885C12R1/865C12R1/78C12R1/72
Inventor 刘乐鞠美庭李维尊邵超峰韩国林夏元东江洋侯其东刘益良
Owner NANKAI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap