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Method for extraction and separation of xanthophyll and flavones in marigold

A technology of lutein and marigold, which is applied in the field of extraction and separation of lutein and flavonoids, can solve the problems of large amount of organic solvent, low product purity, complex extraction process, etc., and achieve the effects of reducing dosage, reducing production cost, and low energy consumption

Inactive Publication Date: 2014-07-23
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to overcome the problems of complex extraction process of lutein and flavonoids in current marigolds, large amount of organic solvent, and low product purity, and provide a two-phase aqueous extraction with simple process flow, low production cost and easy industrialization A method for extracting lutein and flavonoids from marigolds simultaneously with saponification, so that marigold resources can be better developed and utilized

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] A method for extracting and separating lutein and flavonoids, using marigold petal powder as raw material, comprising the following steps:

[0018] (1) Weigh 3.2g potassium hydroxide and add 9.2g water to prepare an aqueous solution, then add 7.6g isopropanol to prepare isopropanol-potassium hydroxide two-phase system, add 0.01g marigold petal powder to isopropanol - In the potassium hydroxide two-phase aqueous system, shake and mix, react at 45°C in the dark for 1 hour, stop stirring, filter the reactant and keep it in the dark;

[0019] (2) After the phase separation is complete, separate the upper and lower phases in step (1), wherein the upper phase is a lutein-rich phase, and the lower phase is a flavonoid-rich phase. Put the two phases in different colorimetric tubes respectively, add 2 times the volume of isopropanol to each, shake and stand in the dark for 4 hours at 0°C, then filter;

[0020] (3) Rotate the filtrate in step (2) and dry it in vacuum to obtain c...

Embodiment 2

[0022] A method for extracting and separating lutein and flavonoids, using marigold petal powder as raw material, comprising the following steps:

[0023] (1) Weigh 3.6g potassium hydroxide and add 8.4g water to prepare an aqueous solution, then add 8.0g isopropanol to prepare isopropanol-potassium hydroxide two-phase system, add 0.03g marigold petal powder to isopropanol - In the potassium hydroxide two-phase aqueous system, shake and mix, react at 50°C in the dark for 1.5 hours, stop stirring, filter the reactant and keep it in the dark;

[0024] (2) After the phase separation is complete, separate the upper and lower phases in step (1), wherein the upper phase is a lutein-rich phase, and the lower phase is a flavonoid-rich phase. Put the two phases in different colorimetric tubes respectively, add 3 times the volume of isopropanol to each, shake and stand in the dark at 2.5°C for 5 hours before filtering;

[0025] (3) Rotate the filtrate in step (2) and dry it in vacuum to...

Embodiment 3

[0027] A method for extracting and separating lutein and flavonoids, using marigold petal powder as raw material, comprising the following steps:

[0028] (1) Weigh 4.0g potassium hydroxide and add 7.6g water to prepare an aqueous solution, then add 8.4g isopropanol to prepare isopropanol-potassium hydroxide two-phase system, add 0.05g marigold petal powder to isopropanol - In the potassium hydroxide two-phase aqueous system, oscillate and mix, react at 55°C in the dark for 2 hours, stop stirring, filter the reactant and keep it in the dark;

[0029] (2) After the phase separation is complete, separate the upper and lower phases in step (1), wherein the upper phase is a lutein-rich phase, and the lower phase is a flavonoid-rich phase. Put the two phases in different colorimetric tubes respectively, add 4 times the volume of isopropanol to each, shake and stand in the dark at 5°C for 6 hours before filtering;

[0030] (3) Rotate the filtrate in step (2) and dry it in vacuum to...

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Abstract

The invention relates to a method for extraction and separation of xanthophyll and flavones in marigold, and belongs to the technical field of extraction of the xanthophyll and flavonoids compounds. According to the method, marigold petal powder is added into an isopropanol-potassium hydroxide two-aqueous-phase system for one-step extraction to separate an upper phase and a lower phase, and a final product is prepared by dealkalization, filtration and evaporation. The method greatly simplifies the process of extraction and separation of the xanthophyll and the flavones, and overcomes the defects of large use amount of organic solvents, high material consumption and high energy consumption. The extraction rate of the xanthophyll in the upper phase reaches 86.73%, and the xanthophyll ester saponification rate reaches 93.98%, and the extraction rate of the flavonoids compounds in the lower phase reaches 78.31%. The method is easy to operate, and green, can realize simultaneous xanthophyll saponification and extraction, and can be used for effective separation of the flavonoids compounds.

Description

technical field [0001] The invention relates to the technical field of extraction of lutein and flavonoids, in particular to a method for extracting and separating lutein and flavonoids from marigold petals. Background technique [0002] Marigold is rich in lutein and flavonoids. Lutein, also known as "plant lutein", is a natural food coloring. Studies have found that lutein is an excellent antioxidant, which has the effects of eye protection, skin care, and cancer prevention. Lutein can also be used as a colorant to adjust the color of food and beverages. It can also be added to poultry feed as a feed additive. Regular consumption of foods rich in lutein can prevent age-related macular degeneration, treat or prevent cataracts, regulate human immunity, and prevent cancer. Flavonoids are a large class of natural products widely distributed in plants, mainly distributed in dicotyledonous plants and gymnosperms. Flavonoids in plants mainly exist in the form of glycosides co...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C403/24A61K36/28
Inventor 王赟吴迎春韩娟胡晓玮倪良刘燕张文莉胡钰涛
Owner JIANGSU UNIV
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