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Novel acacia melanoxylon tissue culture seedling rooting method

A technology of tissue culture seedlings and a new method, which is applied in the field of rooting of Acacia blackwood tissue culture seedlings, can solve problems such as shortening the rooting time, and achieve the effects of shortening the seedling cultivation cycle, saving production costs, and reducing losses

Inactive Publication Date: 2014-07-23
地缘(厦门)生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0002] The development of plant tissue culture and rapid propagation technology has gradually matured so far, but there are still many problems, especially the research on tissue culture and rapid propagation of Acacia blackwood has been at a stagnant stage in recent years, and it has not been possible to find an effective method that can improve The quality of the tissue-cultured seedlings can shorten the seedling cycle and reduce the cost; and the rooting of the tissue-cultured seedlings of Acacia blackwood needs to improve the quality of the root system, shorten the rooting time, and improve the survival rate of transplanting

Method used

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  • Novel acacia melanoxylon tissue culture seedling rooting method
  • Novel acacia melanoxylon tissue culture seedling rooting method
  • Novel acacia melanoxylon tissue culture seedling rooting method

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Experimental program
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specific Embodiment approach

[0031] The tissue-cultured seedlings of Acacia blackwood were derived from the excellent clone seedlings of Acacia blackwood from Jiyuan (Xiamen) Biotechnology Co., Ltd. The experimental site was the domestication room of Jiyuan (Xiamen) Biotechnology Co., Ltd. )°C, humidity 80%-90%.

[0032] Embodiment 1: Acacia blackwood tissue culture seedling takes root

Embodiment 1

[0033] Operation method: First, use inoculation tweezers to poke holes about 1.0~1.5cm deep evenly on the watered transplanting substrate (a mixture of peat soil + sawdust + perlite, the volume ratio is 6:3:1). Small hole. Take out the rootless stem buds of Acacia blackwood cultivated by strong seedlings from the tissue culture bottle, clean the base medium with tap water, cut off the stem buds of Acacia blackwood from the base with an inoculation knife, and cut the leaves with an inoculation knife When there are 3~5 pinnate leaves left near the terminal buds, use tweezers to hold the seedling cuttings and insert them into the substrate and compact the substrate, exposing the terminal buds and leaves by 1.5~2.0cm, and finally place the culture box at 90~135μmol·m -2 ·s -1, the acclimatization room with the light duration of 12~16h can be cultivated.

[0034] Pay attention to the following points after cutting: 1. Spray the surface of the substrate with 0.1% carbendazim or ch...

Embodiment 2

[0036] A. The effect of different transplanting substrates on the open rooting of Acacia blackwood tissue culture seedlings: Consider 4 substrates: ① peat soil; ② peat soil + sawdust (2:1); ③ peat soil + perlite (3:1) ; ④ peat soil + sawdust + perlite (6:3:1). The rootless shoots of Acacia blackwood are not treated with any rooting promoter, and the light intensity for cultivation is 90 μmol·m -2 ·s -1 , the culture photoperiod is 12h / 12h (light / dark).

[0037] Through experiments, it is concluded that the best transplanting substrate suitable for open rooting of excellent clone tissue cultured seedlings of Acacia black wood is peat soil + sawdust + perlite (6:3:1),

[0038] B, the influence of the different water content of transplanting substrate on the open rooting of Acacia safflower tissue culture seedlings: pouring and substrate volume ratio are respectively 1:14, 1:12, 1:10, 1:8 tap water, until water Mix well with the matrix, take soil evenly in each box, do 3 repet...

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Abstract

The invention discloses a novel acacia melanoxylon tissue culture seedling rooting method. The method includes the steps that small holes with the depths of 1.0-1.5 cm are uniformly formed in a watered transplanting substrate through inoculation tweezers in a pricking mode firstly; an acacia melanoxylon rootless stem bud cultured through a strong seedling is taken out of a tissue culture bottle, culture media of a base portion are washed clean with tap water, then the acacia melanoxylon rootless stem bud is cut off from the base portion with an inoculation cutter, and leaves are cut off until 3-5 leatherleaves are left around an apical bud through the inoculation cutter; the acacia melanoxylon rootless stem bud is put in a rooting accelerant to be immersed for a certain period of time, then a small seedling is clamped through the tweezers to be inserted into the transplanting substrate, the transplanting substrate is pressed to be compact, and the exposed apical bud and the leaves exposed are 1.5-2.0 cm away from the upper portion of the transplanting substrate; ultimately, a culture box is placed in a domestication chamber where lighting intensity and lighting time are set. According to the method, root quality is greatly improved, loss caused by pollution is reduced, the survival rate of transplanted seedlings is improved, the seedling cycle is shortened due to the fact that rooting, domestication and transplanting are combined, and production cost is greatly saved.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture rapid propagation. In particular, it relates to a new method for rooting tissue-cultured seedlings of Acacia acacia. Background technique [0002] The development of plant tissue culture and rapid propagation technology has gradually matured so far, but there are still many problems, especially the research on tissue culture and rapid propagation of Acacia blackwood has been at a stagnant stage in recent years, and it has not been possible to find an effective method that can improve The quality of the tissue-cultured seedlings can shorten the period of seedling cultivation and reduce the cost; and the rooting of the tissue-cultured seedlings of Acacia blackwood also needs to improve the quality of the root system, shorten the rooting time, and improve the survival rate of transplanting. Contents of the invention [0003] The purpose of the present invention is to provide a new met...

Claims

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Application Information

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IPC IPC(8): A01G31/00
Inventor 刘丽林文革
Owner 地缘(厦门)生物科技有限公司
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