Preparation method for immobilized microbe oil-spill repairing agent

A technology for immobilizing microorganisms and repairing agents, applied in chemical instruments and methods, immobilizing on or in inorganic carriers, immobilizing on/in organic carriers, etc. qualitative effect

Active Publication Date: 2014-07-16
ZHEJIANG OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The peanut shell-based activated carbon has a porous structure, which can improve mass transfer, and the peanut shell-based activated carbon is added to sodium alginate-CaCl 2 In the embedding method, to improve the mechanical strength of the preparation, there is no report at home and abroad

Method used

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  • Preparation method for immobilized microbe oil-spill repairing agent
  • Preparation method for immobilized microbe oil-spill repairing agent
  • Preparation method for immobilized microbe oil-spill repairing agent

Examples

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Embodiment Construction

[0030] The present invention will be further described in detail below in conjunction with the accompanying drawings and embodiments.

[0031] Implementation Example 1

[0032] A preparation method of an immobilized microbial oil spill restoration agent using sodium alginate-calcium chloride-peanut shell-based activated carbon as an embedded and immobilized material, the specific steps are:

[0033] 1. Preparation of peanut shell-based activated carbon

[0034] 1) Clean, sieve, dry and pulverize raw peanut shells, pass through a 30-mesh sieve, and set aside.

[0035] 2) Weigh the above crushed peanut shells, add it to a zinc chloride activator solution with a mass fraction of 40% (the mass ratio of peanut shells to zinc chloride solution is 1:3), stir and mix, and mix the mixed The feed liquid is soaked at room temperature for about 1 hour;

[0036] 3) Put the impregnated material liquid into a high-temperature muffle furnace, raise it from room temperature to the required ...

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Abstract

A preparation method for an immobilized microbe oil-spill repairing agent is characterized by comprising the following steps: preparing peanut shell-based active carbon; mixing the peanut shell-based active carbon with a seed bacteria liquid to performing adsorption until adsorption is saturated, so as to form a bacteria suspension; adding a sterilized sodium alginate solution with a temperature of 30-40 DEG C into the above bacteria suspension, uniformly mixing, injecting into a CaCl2 solution with a certain concentration by employing an injector, dispersing into balls and performing crosslinking for 12-24 h, so as to obtain immobilized microbe microballoons; and finally flushing the immobilized microbe microballoons with normal saline for 2-4 times to obtain a finished product. According to the preparation method, the biology-based active carbon modified peanut shell is added into a sodium alginate-calcium chloride embedded immobilized carrier for the first time, the mechanical strength and the mass transfer property of the agent are improved, the activity and the degradation efficiency of microbes are improved; also the cost is low, the technology is simple and easy to operate, the reaction conditions are mild, microbes of the prepared repairing agent is not easy to leak, and the prepared repairing agent is good in stability and reusability, has relatively high microbe activity and cell capacity, and has petroleum hydrocarbon degradation rate of 75-92%.

Description

technical field [0001] The invention belongs to the technical field of bioremediation of petroleum and petroleum product pollution, and relates to a preparation method of an immobilized microbial oil spill restoration agent, in particular to an embedding and immobilization agent using sodium alginate-calcium chloride-peanut shell-based activated carbon The preparation method of the material-immobilized microbial oil spill remediation agent is used for the microbial remediation of oceans polluted by petroleum and petroleum products. Background technique [0002] With the development of offshore oil development and marine transportation, oil spill accidents caused by blowouts, oil spills from transport ships, shipwrecks, and oil pipeline leaks have occurred frequently. After the oil spill accident, although some surface oil spills can be removed by physical methods (such as fence recovery, etc.) and chemical methods (spraying oil dispersants, etc.), the remaining petroleum hyd...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/14C12N11/10C02F3/34C02F101/32
Inventor 陈庆国包木太刘梅胡海燕刘艳萍
Owner ZHEJIANG OCEAN UNIV
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