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Lactobacillus johnsonii BS15 and application thereof in preventing and treating non-alcoholic fatty liver

A technology of Lactobacillus johnsonii and BS15, which can be used in the prevention and treatment of non-alcoholic fatty liver, and can solve the problems of unreported probiotics

Active Publication Date: 2014-07-09
SICHUAN AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few reports on probiotics, especially Lactobacillus johnsonii BS15, in the prevention and treatment of NAFLD

Method used

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  • Lactobacillus johnsonii BS15 and application thereof in preventing and treating non-alcoholic fatty liver
  • Lactobacillus johnsonii BS15 and application thereof in preventing and treating non-alcoholic fatty liver
  • Lactobacillus johnsonii BS15 and application thereof in preventing and treating non-alcoholic fatty liver

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1 Acquisition and identification of "Lactobacillus johnsonii CCTCC 2013663" of the present invention

[0022] 1. Isolation of strains

[0023] Take the traditional yogurt from Hongyuan Grassland in Aba Prefecture and dilute it with 0.8% normal saline in equal proportions, and after gradient dilution with PBS, take 100uL and spread it on the MRS plate, and culture it anaerobically at 37°C for 36 hours, and pick a typical single colony for Gram staining observation , and streaked and purified, and then picked a single colony in MRS broth to expand culture, and named as lactic acid bacteria BS15.

[0024] 2. Acid resistance and bile salt resistance experiment of lactic acid bacteria BS15

[0025] Inoculate the 3rd generation of activated lactic acid bacteria BS15 liquid culture medium at pH 3.0 and pH 6.4 at an inoculum of 2% and culture anaerobically at 37°C for 12 h; at the same time inoculate at 0.3% The MRS liquid medium of ox bile salt was cultured anaerobic...

Embodiment 2

[0036] Example 2 Application of "Lactobacillus johnsonii CCTCC 2013663" of the present invention in the prevention and treatment of non-alcoholic fatty liver

[0037] The basic feed in the present invention was purchased from Chengdu Dashuo Biotechnology Co., Ltd.

[0038] The bacterial solution containing "Lactobacillus johnsonii CCTCC 2013663" of the present invention was inoculated with MRS culture solution at a 2% inoculum amount, counted viable bacteria after anaerobic static culture at 37°C for 36 hours, centrifuged at 3000r / min for 15min to retain the bacterial sludge, and buffered with PBS dilute to 2 x 10 8 and 2×10 7 CFU / 0.2mL, store at -20°C for standby, prepare the bacterial solution once a week, activate the bacterial solution in warm water at 25°C before gavage, and use it.

[0039] The high-fat feed in the present invention is prepared into granular feed by 80% basic feed, 0.5% cholesterol, 6.3% lard, 13% egg yolk powder, and 0.2% cholate.

[0040] The prese...

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Abstract

The invention discloses application of ((i) lactobacillus johnsonii( / i)) BS15 with collection number of CCTCC NO:M2013663 in preventing and treating non-alcoholic fatty liver (NAFLD) caused by high-fat food. The lactobacillus johnsonii BS15 remarkably lowers degeneration degree of fat mouse liver fat and apoptosis rate of liver cells.

Description

technical field [0001] The invention relates to a new bacterial strain of the genus Lactobacillus and its application in the prevention and treatment of non-alcoholic fatty liver. Background technique [0002] With the improvement of living standards, obesity and obesity-related non-alcoholic fatty liver disease (NAFLD) have become urgent problems to be addressed by the World Health Organization. Over the past 20 years, NAFLD has grown to 20-30% of adults and 3-10% of children. [0003] At present, studies have found that emodin, VC and VE have a certain improvement effect on NAFLD. However, NAFLD is a chronic metabolic disease, and long-term drug treatment will cause serious damage to the body. Therefore, the development of green and environmentally friendly biological products has become an important technical means to deal with NAFLD. A large number of research reports at home and abroad have confirmed that probiotics have important functions such as anti-cancer, enhan...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K35/74A61P1/16C12R1/225A61K35/747
Inventor 倪学勤曾东辛金鸽曾燕王鹤松祝辉周梦佳李涛潘康成景波
Owner SICHUAN AGRI UNIV
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