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Continuous cropping resistant composite microbial agent and preparation method thereof

A compound microbial bacterial agent and anti-cropping technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems such as unsatisfactory effects, and achieve the goal of promoting photosynthesis, increasing fibrous roots, and promoting rapid growth Effect

Inactive Publication Date: 2014-07-02
山东金利丰生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing microbial agents are not very effective in the process of anti-cropping

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] The activation medium of peptone, beef extract, sodium chloride, agar and sterile water with a mass percentage of 10:3:5:15:1000 and the mass percentage of 200:10:5:400:300:100:10 The production fermentation medium of peptone, beef extract, magnesium chloride, corn flour, sucrose, starch, ferrous sulfate, potassium dihydrogen phosphate and sterile water of 4:2000 was sterilized at 121 ℃ for 30 minutes respectively, and the activation The pH of the culture medium and the production fermentation medium are both 7.5. Bacillus subtilis and Bacillus licheniformis were respectively inoculated in the activation medium, activated and cultivated in a constant temperature shaking incubator at 30°C for 15 hours, and then the cultured strains were Ferment and proliferate in the production fermentation medium for 24 hours, stir once every 2 hours during this period, respectively prepare the Bacillus subtilis bacterial liquid and the Bacillus licheniformis bacterial liquid, respective...

Embodiment 2

[0037] The activation medium and the mass percentage of peptone, beef extract, sodium chloride, agar and sterile water of 10:3:5:15:1000 and the mass percentage of 200:10:5:400:300:100:10 The production fermentation medium of peptone, beef extract, magnesium chloride, corn flour, sucrose, starch, ferrous sulfate, potassium dihydrogen phosphate and sterile water of 4:2000 was sterilized at 121 ℃ for 35 minutes respectively, and the activation The pH of the culture medium and the production fermentation medium are both 7.2. Inoculate Bacillus lateralis and Bacillus megaterium in the activation medium respectively, activate and cultivate them in a constant temperature shaking incubator at 20°C for 36 hours, and then inoculate the cultured bacteria Seeds were fermented and multiplied in the production fermentation medium for 72 hours, during which they were stirred once every 2 hours to obtain Bacillus lateralis bacterium liquid and Bacillus megaterium bacterium liquid respectively...

Embodiment 3

[0041] The activation medium and the mass percentage of peptone, beef extract, sodium chloride, agar and sterile water of 10:3:5:15:1000 and the mass percentage of 200:10:5:400:300:100:10 The production fermentation medium of peptone, beef extract, magnesium chloride, corn flour, sucrose, starch, ferrous sulfate, potassium dihydrogen phosphate and sterile water of 4:2000 was sterilized at 121 ℃ for 33 minutes respectively, and the activation The pH of the culture medium and the production fermentation medium are both 7.4. Bacillus coliformis and Bacillus amyloliquefaciens were respectively inoculated in the activation medium, activated and cultivated in a constant temperature shaking incubator at 28°C for 30 hours, and then the cultured bacteria were The seeds were fermented and multiplied in the production fermentation medium for 65 hours, during which they were stirred once every 2 hours to obtain the Bacillus colloidus bacterium and the Bacillus amyloliquefaciens bacterium r...

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Abstract

The invention belongs to the technical field of biological fertilizers, and in particular relates to a continuous cropping resistant composite microbial agent and a preparation method thereof. The continuous cropping resistant composite microbial agent is prepared from the following raw materials in percentage by weight: 20-60% of composite microbial agent, 2-10% of trace elements, 20-70% of calcium carbonate and 5-10% of drying agent. The preparation method for the continuous cropping resistant composite microbial agent comprises the following steps: (1) culture of bacterial liquid of various bacilli; (2) preparation of the composite microbial agent; (3) preparation of the continuous cropping resistant composite microbial agent. The production process provided by the invention is refined; the product provided by the invention can promote quick growth of crops, adjust the vital movement of the crops, increase both production and income, obviously improve the quality of fruits, and decompose organic matters and toxic and harmful matters so as to form a protective barrier in the root environment of the crops and enhance the stress resistance of the crops.

Description

technical field [0001] The invention belongs to the technical field of biological fertilizers, and in particular relates to a compound microbial bacterial agent for resisting heavy cropping and a preparation method thereof. Background technique [0002] In recent years, with the increasingly serious situation of desertification and desertification in the world, the arable land has become less and less, and the contradiction of more people and less land in our country has become more prominent. Increasing the multiple cropping index (double cropping) is the only way to solve the problem. However, the ensuing cropping diseases such as soil-borne diseases caused large-scale production reduction and extinction of crops. The general incidence rate is 10-30%. Plants often wither, resulting in lack of seedlings and broken ridges. In severe cases, it can reach 80-90%. Repeated cropping disease has become a key topic of research and concern for agricultural experts and farmers, but ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C05G3/00C12R1/125C12R1/10C12R1/07C12R1/11
Inventor 李建林孙希园张洪礼张建国张畔东
Owner 山东金利丰生物科技股份有限公司
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