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Degradable chitosan biomembrane as well as preparation method and application thereof

A biofilm and chitosan technology, applied in the field of biomedical materials, can solve the problems of the inability to exert the characteristics of the biofilm, the inability to use the cell scaffold, and the reduction of the strength of the biofilm, and achieve good natural antibacterial properties and biocompatibility. Sexuality, good economic and social benefits, and the effect of preventing congestion and necrosis

Active Publication Date: 2014-07-02
QINGDAO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The crushing of crab shells undoubtedly destroys the natural reticular and braided forms formed by chitosan itself, and this structure and function of crab shells cannot be utilized. At present, the method of making biofilms with shrimp and crab shells is generally for extracting Chitin and chitosan are synthesized, and a pore-filling agent is added to form a plurality of pores inside the treated biofilm, but the pores are not connected to each other and are sealed pores, and the crushed crab shells When extracting chitin and chitosan, it is necessary to add glacial acetic acid for treatment. During the treatment, glacial acetic acid destroys the chemical bonds of chitin, resulting in a greatly reduced strength of the synthesized biofilm, and the airtightness of the pores cannot seal the biofilm itself. The characteristics of these materials are brought into play, leading to the inability to use guided tissue regeneration and cell scaffolds in the medical field

Method used

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  • Degradable chitosan biomembrane as well as preparation method and application thereof
  • Degradable chitosan biomembrane as well as preparation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0042] Such as figure 2 As shown, a degradable chitosan biofilm comprises a multi-layered structure obtained after decalcification, deproteinization and deacetylation of a complete crab shell, which can be torn off sequentially. A transparent or translucent colorless chitosan multilayer structure film body frame 5 has a plurality of unevenly distributed natural micropores 3 along the thickness and width direction of the chitosan multilayer structure film body frame 5, so The chitosan multilayer structure membrane body frame 5 has a thickness of 1 μm to 12 μm, and the natural micropores 3 have a pore diameter of 1 μm to 50 μm.

[0043] Preparation method: Weigh 50 g of washed and dried Alaska snow crab shells, decalcify with 5% HCl solution with a mass fraction of 10%, and decalcify at 30°C, soak for 1 hour each time, repeat twice, and wash with water twice to medium Sex, then dehydration. Then use 8% NaOH solution and heat in a water bath at 40° C. for 4 hours to deproteini...

Embodiment 2

[0049] Weigh 20 g of crab shells washed and dried, decalcify 3 times with 6% HCl solution, bath ratio 10%, 30° C., soak for 4 hours each time, repeat 3 times after changing the liquid, and wash with water until neutral. Then use 10% NaOH solution and heat in a water bath at 50° C. for 6 hours to deproteinize to obtain a biodegradable chitin biofilm. Then use 12% NaOH solution to deacetylate by heating in a water bath at 99°C for 18 hours to prepare a biodegradable chitosan biofilm. Soak the chitin biofilm or chitosan biofilm in soft water for 4 hours, quickly decompress and stratify, and use a rapid decompression device to make the pressure on the chitosan biofilm from 1 to 12 MPa within 0.01 to 0.1 seconds Rapidly drop to 0.1MPa, and then tear off the chitosan biofilm of corresponding thickness by means of manual splitting. Can produce transparent biofilm or translucent biofilm. With 0.8% KMnO 4 solution, with a bath ratio of 10%, soaked at 60° C. for 6 hours for decoloriz...

Embodiment 3

[0051] Wash the hairy crab shells, decalcify with 7% HCl solution, power 1000w, ultrasonic for 180s, repeat 3 times. After washing, use 12% NaOH by mass fraction, heat in a water bath at 100°C for 16 hours, deproteinize, and then wash with water until neutral to obtain a degradable chitin biofilm; use 15% NaOH by mass fraction for this degradable chitin biofilm solution, deacetylated by heating in a water bath at 100°C for 24h, and then washed to neutrality to obtain a chitosan biofilm; 4 Soak in the solution for 6 hours to decolorize, then apply 5% HCl solution and 10% NaOH solution successively and put them in a microwave oven for 6 minutes, and then perform secondary decalcification and deproteinization treatments respectively. Through the method of embodiment 1, 2, the transparent or translucent chitosan biofilm that thickness is 12 μ m, and pore is 50 μ m is prepared by rapid decompression and manual splitting method.

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Abstract

The invention discloses a degradable chitosan biomembrane as well as a preparation method and an application thereof. The degradable chitosan biomembrane comprises a chitosan multi-layer structural membrane main body frame which is provided with nature micropores and a multi-layer structure. The chitosan biomembrane is prepared by decalcifying, deproteinizing, removing acetophenetidin and manually layering in sequence. The biomembrane is degradable. Compared with an aporate structural biomembrane, the chitosan biomembrane serving as a tissue adhesion preventing membrane has the advantages that the porous structure is beneficial for the exchange of nutrient substances, and the biological tissue below the membrane can be prevented from extravasated blood and necrosis; the chitosan biomembrane serving as a guided tissue regeneration membrane has the beneficial effects that the porous structure is beneficial for the adhering and growth of osteoblast and osteocyte; the membrane does not need the secondary surgery for taking out and is the membrane material with high biocompatibility and safety and without toxicity and irritation, and the membrane material can be degraded and absorbed; the unique surface structure characteristics enable the effective separation of surgical wound and surrounding tissue, and thus the surgical wound can be protected effectively.

Description

technical field [0001] The invention belongs to the field of biomedical materials, and in particular relates to a chitin or chitosan biofilm used for medicine and cosmetics, a preparation process and application. Background technique [0002] In the coastal areas, a huge amount of crab aquatic products is caught every year. With the increase of freshwater and seawater crab culture products in our country, there are a large number of by-products of crab shells every year. After the crab meat is extracted by aquatic product companies, only a small part of the crab shells are simple. Dried and crushed for the feed industry as an additive to feed calcium sources. Shrimp and crab shells are solid wastes produced by the aquaculture industry. With the development of my country's seafood and artificially cultured shrimp and crab industries, the amount of these wastes is increasing, and has caused serious environmental pollution. If it is recycled for the production of high value-ad...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L31/06A61L31/14A61L31/16A61L27/20A61L27/54A61L27/56
Inventor 陈韶娟金光硕李扬杜西超
Owner QINGDAO UNIV
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