Probiotics micro-ecological tablet and preparation method thereof
A technology of probiotic microecology and probiotics, which is applied in food preparation, pharmaceutical formulations, medical raw materials derived from bacteria, etc., can solve the problems of insufficient health care effects and decreased bacterial vitality, and achieve enrichment of intestinal flora, The effect of diverse strains and storage stability
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Embodiment 1
[0027] 1) Preparation of probiotic starter
[0028] ① Preparation of bifidobacteria
[0029] Inoculate the frozen Bifidobacterium adolescent powder into MRS lactose medium, culture at 37°C for 24 hours, and the microscopic examination conforms to the standard form of bifidobacteria, and the growth state is good. Inoculate 3% of the inoculum into MRS lactose medium For activation, culture at 37°C for 24 hours and save for later use.
[0030] ② Preparation of Kefir starter
[0031] Use skim milk powder to make 10% skim milk solution, after sterilizing at 115°C for 30 minutes, cool to 25°C, inoculate kefir grains with 0.1% of the volume of skim milk, ferment at 23°C for 18 hours and store for later use .
[0032] 2) Mixed fermentation
[0033] Take concentrated whey protein 10g, glucose 6.7g, yeast powder 3.8g, calcium carbonate 3.4g, magnesium sulfate 0.2g, manganese sulfate 0.015g, whey powder 14g, inulin 2.5g, ginseng polysaccharide 0.5g, soybean oligosaccharide 1g, add d...
Embodiment 2
[0040] 1) Preparation of probiotic starter
[0041] ① Preparation of bifidobacteria
[0042] Inoculate the frozen Bifidobacterium adolescent powder and Bifidobacterium infantis powder into MRS lactose medium respectively, culture at 37°C for 24 hours, and the microscopic examination conforms to the standard form of Bifidobacterium, and the growth state is good. 5%, were inoculated in MRS lactose medium for activation, 37 ℃, cultured for 20h. Mix Bifidobacterium adolescentis and Bifidobacterium infantis in a volume ratio of 1:1, and save for future use.
[0043] ② Preparation of Kefir starter
[0044] Use skim milk powder to make 10% skim milk solution, after sterilizing at 115°C for 30 minutes, cool to 23°C, inoculate kefir direct-throwing starter at 0.3% of the volume of skim milk, and ferment at 26°C Save for later use after 16 hours.
[0045] 2) Mixed fermentation
[0046]Take concentrated whey protein 18g, glucose 5g, yeast powder 4.29g, calcium carbonate 4.0g, magnes...
Embodiment 3
[0053] 1) Preparation of probiotic starter
[0054] ① Preparation of bifidobacteria
[0055] The frozen Bifidobacterium adolescent powder, Bifidobacterium longum powder and Bifidobacterium infantis powder were respectively inoculated in MRS lactose medium at 37°C. After culturing for 24 hours, the standard form of Bifidobacterium was found by microscope inspection. The growth state is good, according to the inoculum size of 6%, inoculated in MRS lactose medium for activation, 37 ℃, cultured for 20h. Mix Bifidobacterium adolescentis, Bifidobacterium longum and Bifidobacterium infantis in a volume ratio of 2:1:1, and store for later use.
[0056] ② Preparation of Kefir starter
[0057] Use skim milk powder to prepare 10% skim milk solution, after sterilizing at 115°C for 20 minutes, cool to 20°C, inoculate 0.5% of the volume of skim milk with kefir starter, and ferment at 28°C Save for later use after 14 hours.
[0058] 2) Mixed fermentation
[0059] Take concentrated whey ...
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