Method for identifying natural musk and muskone

Abstract

The invention discloses a method for identifying natural musk and muskone. The method comprises the following step: with hexacosanoic acid and octacosanoic acid as reference substances, performing qualitative identification by adopting an ultrahigh performance bonded-phase chromatography-tandem mass spectrometry method. The method disclosed by the invention is used for identifying the natural musk and the muskone; a sample is simple in pretreatment, and rapid, accurate, and environmentally friendly in detection.

Description

technical field [0001] The invention belongs to the field of drug analysis, in particular to a method for identifying natural musk and artificial musk. Background technique [0002] Natural musk (Moschus) is a precious and rare Chinese medicinal material. It is the dry secretion in the mature male sachet of the deer animal Moschus berezovskii Flerov, Moschus sifanicus Przewalski or the original musk deer Moschus moschiferus Linnaeus. The musk deer is a first-class protected wild animal in the country. The source and output of natural musk are very limited, and most of the current clinical use is artificial musk. [0003] Artificial musk is a national first-class new drug developed according to the chemical components of natural musk. The types and contents of its chemical components are very similar to those of natural musk. . Compared with artificial musk, natural musk also contains many unknown trace components, and some of these trace components themselves have biologic...

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Problems solved by technology

[0005] Liang Ying and Wang Xiaogen (preliminary analysis of natural musk and artificial musk by GC-MS method, "New Drugs and Clinical Pharmacology of Traditional Chinese Medicine", 2005, Volume 16, No. 3, p204-205) reported that musk pyridine, 3-methylcyclo Tridecone is used as a specific ingredient, and the GC-MS method is used to identify natural musk and artificial musk. This method can accurately identify natural musk and artificial musk, but there are the following deficiencies: the extraction reagent uses benzene, which is highly toxic; the pretreatment time of the test product Long, more than 1.5h; long separation time, the retention time of specific components is more than 20min

[0006] Zhou Jian et al. (Using infrared spectroscopy to identify the authenticity of traditional Chinese medicine musk, Spectroscopy and Spectral Analysis, 2010, Volume 30, No. 9, p2368-2371) reported the use of Fourier transform-infrared spectroscopy (FTR-IR) Research on natural musk and artificial musk, found that the infrared spectra of the two are very similar, have common absorption peaks and most of the intensity is consistent, it is difficult to identify; although using the comparison and analysis of the second order derivative spectrum, it is found Musk has differences in some peaks, but the differences are small and it is difficult to distinguish the two definitely

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