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Preparation method of platinum nanoclusters and application of platinum nanoclusters in tumor imaging agents and apoptosis agents

A platinum nano-imaging agent technology, applied in echo/ultrasound imaging agents, MRI/MRI contrast agents, anti-tumor drugs, etc., to reduce cytotoxicity, broad medical application prospects, and enhance targeting effects

Active Publication Date: 2014-06-18
SOUTHEAST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the process of photothermal therapy, the biocompatibility of nanomaterials is always the biggest challenge. Platinum nanoclusters have excellent biocompatibility. Imaging-guided photothermal therapy and other reports

Method used

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  • Preparation method of platinum nanoclusters and application of platinum nanoclusters in tumor imaging agents and apoptosis agents
  • Preparation method of platinum nanoclusters and application of platinum nanoclusters in tumor imaging agents and apoptosis agents
  • Preparation method of platinum nanoclusters and application of platinum nanoclusters in tumor imaging agents and apoptosis agents

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1 A rapid one-step method for the preparation of platinum fluorescent nanoclusters based on reduced glutathione:

[0028] 1) Prepare reduced glutathione (GSH) and platinum salt solution (chloroplatinic acid is used in this example).

[0029] 2) Mix an aqueous solution of reduced glutathione with a concentration of 0.05 mol / L and an aqueous solution of ascorbic acid (vitamin C) with a concentration of 0.05 mol / L, and then add an aqueous solution of platinum reagent with a concentration of 0.05 mol / L, the volume ratio of the three solutions It is 1:1:1, mix well, then heat in a water bath at 37°C, and the reaction is complete when the solution turns orange.

[0030] The platinum fluorescent nanoclusters prepared in this example have good stability and a uniform particle size of about 1.2-2.0 nm.

[0031] The fluorescence spectrum of the platinum fluorescent nanoclusters prepared in this example is as follows figure 1 As shown, curve 1 in the figure is the flu...

Embodiment 2

[0038] Example 2 A rapid one-step synthesis method for platinum fluorescent nanoclusters based on reduced glutathione:

[0039] 1) Prepare reduced glutathione (GSH) and platinum salt solution (potassium chloroplatinite is used in this example).

[0040] 2) Mix the reduced glutathione aqueous solution with a concentration of 0.1 mol / L and the ascorbic acid (vitamin C) aqueous solution with a concentration of 0.01 mol / L, and then add a platinum reagent aqueous solution with a concentration of 0.05 mol / L, the volume ratio of the three solutions It is 2:2:1, the three are fully mixed, and then heated in a water bath at 37°C, and the reaction is complete when the solution turns orange.

[0041] The platinum fluorescent nanoclusters prepared in this example have good stability and a uniform particle size of about 1.4-2.2 nm.

[0042] The emission wavelength of the platinum fluorescent nanoclusters prepared in this example is around 455nm, which is different from the Pt fluorescen...

Embodiment 3

[0043] Example 3 A rapid one-step synthesis method for platinum fluorescent nanoclusters based on reduced glutathione:

[0044] 1) Prepare reduced glutathione (GSH) and platinum salt solution (ammonium chloroplatinite is used in this example).

[0045] 2) Mix the reduced glutathione aqueous solution with a concentration of 0.01 mol / L and the ascorbic acid (vitamin C) aqueous solution with a concentration of 0.1 mol / L, and then add a platinum reagent aqueous solution with a concentration of 0.05 mol / L, the volume ratio of the three solutions It is 2:1:1, the three are fully mixed, and then heated in a water bath at 37°C, and the reaction is complete when the solution turns orange.

[0046] The platinum fluorescent nanoclusters prepared in this example have good stability and a uniform particle size of about 1.4-2.3 nm.

[0047] The emission wavelength of the platinum fluorescent nanoclusters prepared in this example is around 457nm, which is different from the Pt fluorescent...

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Abstract

The invention discloses a preparation method of platinum nanoclusters and application of the platinum nanoclusters in tumor imaging agents and apoptosis agents. The preparation method comprises the following specific steps of: mixing a platinum salt solution and ascorbic acid (VC) and reduced glutathione (GSH), and heating in a water bath, wherein the synthesis speed and the particle size of the platinum nanoclusters are controlled by adjusting the pH and temperature of a solution and a ratio of components. The prepared platinum nanoclusters have a small particle size, good permeability, and excellent biocompatibility and stable fluorescence characteristics, can be used for high spatial and temporal resolution imaging on tumors and other lesion sites, and can effectively promote tumor cell apoptosis and tumor tissue ablation; the method and the application are simple and easy to implement, the toxicity is low, the sensitivity is high, the multi-mode real-time dynamic monitoring can be carried out, and the medical clinical application value and prospect are broad.

Description

technical field [0001] The invention relates to a preparation method of platinum nano-cluster and its application in tumor imaging agent and apoptosis agent. The invention uses the prepared platinum nanocluster preparation as a probe to accurately guide tumors and other lesion sites, and can be further combined with imaging-guided photothermal therapy to achieve highly sensitive diagnosis and treatment of tumors. Background technique [0002] At present, the death of malignant tumor (cancer) still ranks first among various causes of death in the world, and it has become a major killer of people's lives today. Since the early symptoms of cancer are not obvious, it is difficult to be detected early, so the early diagnosis and treatment of cancer is still one of the most important medical problems to be solved urgently, and it is very critical to the cure of cancer. [0003] In recent years, with the development of nanoscience and technology, nanomaterials have attracted much...

Claims

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Application Information

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IPC IPC(8): A61K49/00A61K49/06A61K49/22A61K49/18A61K41/00A61K47/18A61K47/22A61P35/00A61P35/02
Inventor 王雪梅陈东华革伟高生平
Owner SOUTHEAST UNIV
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