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Method for in vitro and rapid propagation of Dieffenbachia tiger-eye with flower stalk as explant

A technology of explants and explants, which can be used in botany equipment and methods, horticultural methods, plant regeneration, etc., can solve unfavorable subculture, low reproduction coefficient, and limit the application of tissue culture technology of tiger eye. problems, to achieve the effect of low cost, efficient regeneration system, and low variation of regenerated plants

Inactive Publication Date: 2016-02-17
BEIJING UNIV OF AGRI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the previous experiments on the evergreen tissue culture have been initially successful, bulbs are usually used as explants to induce callus. Because bulbs are used as explants to grow leaves and roots faster, it is not conducive to subculture. lower
This has limited the application of the tissue culture technology of evergreen evergreen in actual production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] 1. Inoculation of explants and induction of callus: cut off the young flower stalk at the lower end of the tiger's eye flower, put the young flower stalk into a sterile ultra-clean bench, and use 75% (volume / volume) alcohol Soak for 1 minute, soak in 0.2% (mass / volume) mercuric solution for 5 minutes, and rinse with sterile water 5 times. The young flower stalks were cut into small pieces with a length of 3 to 5 mm, and inoculated on MS medium containing 6-BA 2 mg / L, IBA 0.15 mg / L, sucrose 3.5% (mass / volume), and pH 6.2. 20 ℃ ~ 30 ℃ 4 ~ 6 hours of fluorescent light every day, light intensity 1500 ~ 2000Lux. Subculture once every 25 days, and differentiation can be induced when yellow-green callus grows.

[0013] 2. Induction of adventitious buds: transfer the yellow-green callus to MS containing NAA (0.15mg / L)+KT (2.0mg / L)+agar (7g / L)+4.0% sucrose (mass / volume) Induction differentiation medium, pH 6.1, subculture once every 40 days, 20 ℃ ~ 30 ℃ daily fluorescent light...

Embodiment 2

[0017] 1. Inoculation of explants and induction of callus: cut off the young flower stalk at the lower end of the tiger's eye flower, put the young flower stalk into a sterile ultra-clean bench, and use 75% (volume / volume) alcohol Soak for 2 minutes, soak with 0.1% (mass / volume) mercuric chloride solution for 10 minutes, and rinse with sterile water for 5 times. The young flower stalks were cut into small pieces with a length of 3-5 mm, and inoculated in MS medium containing 6-BA 2.2 mg / L, IBA 0.1 mg / L, sucrose 3.8% (mass / volume), and pH 5.8 On, 20 ℃ ~ 30 ℃ 4 ~ 6 hours of fluorescent light every day, light intensity 1500 ~ 2000Lux. Subculture once every 28 days, and differentiation can be induced even when yellow-green callus grows.

[0018] 2. Induction of adventitious buds: transfer the yellow-green callus to MS containing NAA (0.4 mg / L) + KT (2.0 mg / L) + agar (5 g / L) + 3.5% sucrose (mass / volume). Induction differentiation medium, pH 5.5, subculture once every 45 days, 20 ...

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Abstract

The invention belongs to flower propagation methods and in particular relates to rapid vegetative propagation method of flowers. A small and tender flower stalk of ornithogalum saundersiae serves as an explant, and the dedifferentiation rate and differentiation rate can be over 70-75 percent. More than 300 cluster buds can be differentiated by performing repeated subculture on callus induced by the explant. The rooting rate of the cluster buds can be over 90 percent, and the transplanting survival rate in a proper environment is over 95 percent. Therefore, ornithogalum saundersiae plants with neat appearance are propagated in a large scale in a relatively short time, and the regeneration plant variation is low; the regeneration system is highly efficient; the average cost of single regeneration plants is extremely low, and the method is suitable for large-scale high-efficiency rapid propagation of ornithogalum saundersiae and is greatly applied to industrialized production.

Description

technical field [0001] The invention belongs to a flower propagation method, in particular to a method for asexual rapid propagation of flowers. Background technique [0002] Tiger eye evergreen, alias sea onion, bird milk flower. It is a perennial herb of the genus Liliaceae, native to southern Africa. Bulbs ovoid, green, up to 10 cm in diameter. There are 5-6 leaves, band-shaped or elongated-lanceolate, 30-60 cm long and 2.5-5 cm wide. It also has high ornamental value as an ornamental flower. Tiger's Eye Evergreen contains tiger's-eye saponin (OSW-1 for short), and its in vitro anticancer activity was found to be ten times stronger than clinically used anticancer drugs such as paclitaxel, doxorubicin, and camptothecin in physiological activity screening experiments. times or even a hundred times. And it is not toxic to normal cells. The saponin OSW-1 isolated from Dieffenbachia has obvious anticancer activity against lung cancer and breast cancer, which is 10-100 tim...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 赵和文崔金腾张克中李月华王杰陈建梅
Owner BEIJING UNIV OF AGRI
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