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Lycium exsertum tissue culture and rapid propagation method

A tissue culture and rapid technology, applied in the field of plant tissue culture, can solve problems such as expansion and reproduction, and achieve the effect of improving survival rate, reducing cost and good effect.

Active Publication Date: 2014-06-11
甘肃鼎鑫农业科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The primary problem faced after the introduction of this species of wolfberry is to expand and reproduce

Method used

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  • Lycium exsertum tissue culture and rapid propagation method
  • Lycium exsertum tissue culture and rapid propagation method
  • Lycium exsertum tissue culture and rapid propagation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] A. Explant treatment

[0033] Collect the young stems of Lycium exsertum as explants, cut off the leaves and leave a small amount of petioles, cut each 2-3 buds into a section, wash the stems with washing powder to remove stains, and then rinse them with tap water for 5 minutes. -10min. Then put it into an ultra-clean workbench for disinfection, cut off both ends of the stem after disinfection, and put it in a petri dish for later use.

[0034] B. Primary culture

[0035] 30 bottles were inoculated for each treatment, and the growth status of the explants was recorded every 7 days after inoculation.

[0036] Based on MS medium, add 0.05mg / L NAA, 3% sucrose and 4g agar, and adjust the pH of the medium to 5.8 with NaOH or HCl. The culture conditions are 3000 lx full light for 24 hours, a temperature of 25°C, and a humidity of 70%RH. The proportion of adventitious bud growth reached 93.3%.

[0037] C. Subculture

[0038] The culture materials were single seedlings or...

Embodiment 2

[0044] The difference from Example 1 is that the rooting culture steps are different: use the leaves of the cultured plants in step B as the material to carry out cluster bud proliferation culture, take 1 / 2MS medium as the base medium, add 1.0 mg / L of 6-benzylaminoadenine, 0.5 mg / L indolebutyric acid (IBA); the culture condition is to first culture at 25°C in dark for 7 days, then at 25°C, with a light intensity of 3000lx full light, and a humidity of 70%RH until cluster buds grow.

Embodiment 3

[0046] The difference from Example 1 is that the steps of rooting culture are different: the leaves of the cultured plants in step C are used as materials to carry out cluster bud proliferation culture, and 1 / 2MS medium is used as basal medium, and 1.0 mg / L of 6-benzylaminoadenine, 0.5 mg / L indolebutyric acid (IBA); the culture condition is to first culture at 25°C in dark for 7 days, then at 25°C, with a light intensity of 3000lx full light, and a humidity of 70%RH until cluster buds grow. Under this condition, the multiplication coefficient of cluster buds is the highest, the growth is the most vigorous, and the multiplication coefficient can reach more than 5.0.

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Abstract

The invention discloses a lycium exsertum tissue culture and rapid propagation method, aiming at solving the propagation problem of introduced lycium exsertum. The method comprises the following steps: explant culturing, primary culturing, subculturing, rooting culturing, seedling hardening and transplanting. The lycium exsertum tissue culture and rapid propagation method has the advantages that only is 6-Benzylaminopurine blended with indole-3-acetic acid when subculturing is performed on the lycium exsertum, so that the influence of hormone accumulation on multiple subculturing is reduced, and the cost is also lowered; no hormone is added in the adopted culture medium when rooting culturing is performed on subcultured seedlings of lycium exsertum, so that the preparation of the culture medium is simplified, the cost is lowered, and the effect is good; peat, vermiculite and perlite in the transplanting medium are mixed according to the ratio of 1:1:1, so that water retaining and heat preservation effects can be achieved in the transplanting process, and the survival rate of transplanting can be increased.

Description

technical field [0001] The invention belongs to the field of plant tissue culture, in particular to a Lycium exsertum tissue culture and rapid propagation method. Background technique [0002] Solanaceae Lycium barbarum Lycium exsertum It is a plant of the Solanaceae Lycium genus introduced from the United States, mainly distributed in the Arizona desert area, and its evolution level is higher than that of Chinese wolfberry. In terms of reproductive evolution, hermaphrodites and self-incompatible diploids have evolved to self-compatible polyploids, while my country is still in the evolutionary stage of self-incompatible diploids. The introduction of this kind of wolfberry has great significance for the improvement of germplasm resources of Chinese wolfberry. The primary problem faced after the introduction of this kind of wolfberry is to expand and reproduce. Contents of the invention [0003] The object of the present invention is to provide a kind of Lycium exsertum t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01G31/00
Inventor 李捷冯丽丹王有科张宝琳张广忠蔡国军
Owner 甘肃鼎鑫农业科技有限公司
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