Multi-strain microorganism leavening agent and preparation method thereof
A microbial starter and multi-strain technology, which is applied in the field of multi-strain microbial starter and its preparation, can solve the problems of unreachable production and insufficient supply, and achieve the effects of improving quality, increasing production and increasing production
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Embodiment 1
[0014] Bacillus subtilis, Bacillus megaterium, Aspergillus niger, Streptococcus lactis, Candida genus, and Corynebacterium glutamicum were respectively activated and cultured on slant surfaces, wherein the culture medium of Bacillus subtilis was starch 0.15%+ Glucose 0.5% + urea 0.1% + dipotassium hydrogen phosphate 0.3% + potassium dihydrogen phosphate 0.15% + magnesium sulfate 0.05% + yeast extract 0.02% + ferric chloride 0.01% + calcium carbonate 0.01% + soybean meal 1%; Bacillus megaterium The culture medium is 5.0 grams of beef extract, 8.0 grams of peptone, 2.0 grams of glucose, 0.5 grams of magnesium sulfate, 3.0 grams of sodium chloride, 1.0 grams of dipotassium hydrogen phosphate, 1000ml of water, pH = 7.0-7.5, temperature 34 ℃; vegetable milk The culture medium for Bacillus is 5.0 grams of beef extract, 2.5 grams of yeast powder, 10.0 grams of glucose, 0.5 grams of Tween-80, 1.0 grams of ammonium citrate, 2.5 grams of sodium acetate, 0.05 grams of manganese sulfate, 0...
Embodiment 2
[0017] Bacillus subtilis, Bacillus megaterium, Aspergillus niger, Streptococcus lactis, Candida aeruginosa, and Corynebacterium glutamicum are the same as in Example 1 for strain activation and slant culture.
[0018] The ratio of each microbial strain according to the quantity and percentage: Bacillus subtilis 10%, Bacillus megaterium 11%, Lactobacillus plantarum 15%, Streptococcus lactis 17%, Candida genus 28%, Corynebacterium glutamicum 19% , mixed into a conical flask, placed in a shaker, cultured at 28°C for 3 days, controlled at pH 6.5-7.0, so that the number of bacteria reached 600-800 million / ml, and the multi-strain Microbial starter 2.
Embodiment 3
[0020] Bacillus subtilis, Bacillus megaterium, Aspergillus niger, Streptococcus lactis, Candida aeruginosa, and Corynebacterium glutamicum are the same as in Example 1 for strain activation and slant culture.
[0021] The ratio of each microbial strain according to the quantity and percentage: Bacillus subtilis 13%, Bacillus megaterium 16%, Lactobacillus plantarum 11%, Streptococcus lactis 12%, Candida genus 24%, Corynebacterium glutamicum 24% , mixed into a conical flask, placed in a shaker, cultured at 28°C for 2 days, controlling the pH at 6.5-7.0, so that the number of bacteria reached 600-800 million / ml, and the multi-strain Microbial starter 3.
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