Micro-fluidic chip for multi-index detection

Abstract

The invention discloses a micro-fluidic chip for multi-index detection. The micro-fluidic chip comprises a substrate and a cover plate which is matched with the substrate in a sealed mode, wherein a through hole is formed in the center of the micro-fluidic chip; one or more wave-shaped main channels are formed in the substrate; one end of each main channel is communicated with a sample introduction hole formed in the substrate, and the other end of each main channel is communicated with an air outlet in the substrate; wave troughs of the main channels are arranged away from the direction of the through hole; wave crests of the main channels are arranged close to the direction of the through hole; any one wave trough of the main channel is communicated with a reaction pool through a connecting pipeline; a buffer pool is arranged on the connecting pipeline. The micro-fluidic chip can be detected by instruments through fluorescence, turbidity and development or is directly observed by naked eyes, can be detected in the reaction process in real time and also can be detected after the reaction is ended.

Description

technical field [0001] The invention relates to a microfluidic chip for multi-index detection, which belongs to the field of microfluidic chips and the field of biological detection. Background technique [0002] Microfluidic chips are currently a hot field in the development of Micro Total Analysis Systems (μ-TAS). It uses chips as an operating platform, and through the combination of technologies such as biology, chemistry, and drug screening, it completes the The whole process including loading, separation, reaction, detection, etc. In recent years, with the rapid development of biochip technology, microfluidic chips are playing an increasingly important role in the fields of life sciences, analytical chemistry and biomedicine. [0003] In order to detect samples efficiently, quickly, and with high throughput, the chip is required to have multiple sets of reaction wells and an effective delivery method for delivering samples to each reaction well. Generally, the microfl...

AI Technical Summary

Problems solved by technology

The design of the main channel, that is, the design of the main channel with the same width between the mountain and the valley still fails to solve the problem of the uniformity of liquid dosing in each sampling hole; in addition, the design of the valley is too wide than the mountain, resulting in There is a problem of air bubbles in the main flow

In addition, by adding the waste liquid chamber to carry excess solution, although the consistency of the liquid volume distributed in each sample hole is achieved to a certain extent, it cannot guarantee that the liquid is always full of the sample hole during the reaction process, and it needs to be filled in the microfluidic pipeline. Local surface modification treatment is carried out inside, that is, the hydrophilic treatment of the inner surface of the branch flow channel connected to the sample hole, and the hydrophobic treatment of the inner surface of the branch flow channel connected with the waste liquid chamber that collects the remaining solution, which increases the complexity of chip manufacturing. Sex, processing cost and processing difficulty

Images