Application of methyl benzenediol in phellinus igniarius to resisting H5N1 avian influenza virus

A technology of cresyldiol and Phylophora spp., applied in antiviral agents, applications, preparation of organic compounds, etc.

Inactive Publication Date: 2014-05-28
QINGDAO AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003]At present, the types of anti-avian influenza virus drugs mainly focus on monoclonal antibodies, which are single and have certain limitations

Method used

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  • Application of methyl benzenediol in phellinus igniarius to resisting H5N1 avian influenza virus

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0031] (1) The fermentation medium formula is in grams / 100 milliliters:

[0032] Corn Starch 1% Glucose 1%

[0033] Peptone 0.1% Yeast Extract 0.1%

[0034] Magnesium sulfate 0.1% Potassium dihydrogen phosphate 0.01%

[0035] (2) Inoculate the Phyloporus spp. strains into the Erlenmeyer flask with the liquid medium in a conventional method, with a temperature of 25° C., a shaking flask rotation speed of 110 r / min, and pH 7 conditions, and vibrate for 7 days; cultivate When the pH value drops to 3, inoculate the seeds in the shake flask into the culture solution of a 50L fermenter, stir at a temperature of 25°C, a fermenter pressure of 0.1 kg / cm2, a pH of 3, and an air flow of 0.5-1.1vvm. Under the condition of a speed of 100 r / min, after culturing for 7 days, the crude extract of Pheronotus can be prepared by using the whole liquid fermented with the mycelia of Ph.

[0036] (3) Take the fermented whole solution of Phylophorus mycelium obtained in step (2), and concentrate i...

example 2

[0048] (1) The fermentation medium formula is in grams / 100 milliliters:

[0049] Corn Starch 3% Glucose 2%

[0050] Peptone 0.5% Yeast Extract 0.5%

[0051] Magnesium sulfate 0.5% Potassium dihydrogen phosphate 0.05%

[0052] (2) Inoculate the Phylophorus spp. strain into the Erlenmeyer flask with liquid medium in a conventional way, with a temperature of 30° C., a shaker flask rotation speed of 180 r / min, and pH 6 conditions, and vibrate for 15 days; during the cultivation When the pH value drops to 2.5, inoculate the seeds in the shake flask into the culture solution of a 50L fermenter, with a temperature of 30°C, a fermenter pressure of 0.2 kg / cm2, pH 3, an air flow of 0.5-1.1vvm, and a stirring speed Under the condition of 180 rev / min, after culturing for 15 days, the crude extract of Pheronotus can be prepared by using the fermented whole solution of Phyloporea mycelium.

[0053] (3) Take the fermented whole solution of the Phlomophorus mycelium obtained in step (2), and...

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Abstract

The invention discloses methyl benzenediol with an avian influenza-resisting activity, which is separated from fermentation broth and fruiting bodies of phellinus igniarius (LexFr) Quel (phellinus linteus (BerketCurt) Teng, phellinus baumii and phellinus hartigii (AlleschetSchnabl) Imaz). The invention firstly discloses a new activity of methyl benzenediol and proves that the methyl benzenediol has an avian influenza-resisting action. Compared with the application of a general compound, the method has the advantages that the special new activity of methyl benzenediol is found, and the methyl benzenediol has the action of resisting the avian influenza virus; a new drug is added for treatment of avian influenza in poultry production.

Description

technical field [0001] The present invention relates to a method for extracting and preparing active substances from Phylonotus spp., which is resistant to avian influenza H5N1 virus, especially the S. General Microbiology Center of the Preservation Management Committee", the deposit number is CGMCC No.8108, the taxonomic name is Phellinus igniarius, and the deposit address is No. 3, No. 1, Beichen West Road, Chaoyang District, Beijing, Institute of Microbiology, Chinese Academy of Sciences. Background technique [0002] Hydroquinone can volatilize with steam and is a strong reducing agent. It can burn when exposed to open flames. It emits toxic and corrosive odors when exposed to high heat. It can cause poisoning through the skin and can sublimate. It has a stimulating effect on the central nervous system and respiratory system. Rat oral LD50 is 3890mg / kg, which is different from resorcinol and hydroquinone. [0003] At present, the types of anti-avian influenza virus dr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/05A61P31/16C07C39/08C07C37/70A01G1/04
Inventor 赵晨宋爱荣孔超黄芳梁大勇
Owner QINGDAO AGRI UNIV
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