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Method for efficiently and quickly producing bletilla striata seedling

A Bletilla striata and fast technology, applied in the field of efficient and rapid production of Bletilla striata seedlings, can solve the problems of long production cycle, low seed germination rate, slow growth and development of protocorm, etc. developmental effects

Inactive Publication Date: 2014-05-28
NANJING INST FOR THE COMPREHENSIVE UTILIZATION OF WILD PLANTS CHINA COOP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the existing rapid propagation of seedlings, the seeds are usually sown on a solid medium during the entire production process, and then undergo stages such as differentiation and rooting, which lead to problems such as low seed germination rate, slow growth and development of protocorms, and long production cycles.

Method used

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  • Method for efficiently and quickly producing bletilla striata seedling
  • Method for efficiently and quickly producing bletilla striata seedling
  • Method for efficiently and quickly producing bletilla striata seedling

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Soak the Bletilla striata capsules in alcohol for 35 seconds, then soak them in an aqueous solution of sodium hypochlorite with an available chlorine concentration of 0.8% for 10 minutes, and then rinse them with sterile water for 3 to 4 times.

[0021] Cut the sterilized capsules, sow the Bletilla striata seeds in the liquid medium, and culture them with vibration; see the growth condition of the seeds after germination. figure 1 A; At the same time, the seeds were sown on the solid medium as a control group; the liquid medium was composed of: 1 / 4MS medium + 0.6 mg / L of 6-benzylpurine + 3.1 mg / L of naphthaleneacetic acid + 150 grams of potato juice / L + sucrose 30 g / L + pH 6.2; the composition of the solid medium is 7 g / L of agar, and the other components are the same as the liquid medium; the liquid culture conditions are 19 degrees Celsius, 220 rpm, and 2200 lux light 1. Under the condition of 6 hours / day of light time, shake culture for 19 days, the seeds germinate...

Embodiment 2

[0026] Soak the Bletilla striata capsules in alcohol for 35 seconds, then soak them in 0.5% sodium hypochlorite aqueous solution for 15 minutes, and then rinse them with sterile water for 3 to 4 times.

[0027] Cut the sterilized capsules, sow the Bletilla striata seeds in the liquid medium, and culture them with vibration; see the growth condition of the seeds after germination. figure 1 B; The composition of the liquid medium is: 1 / 4MS medium + 0.3 mg / L of 6-benzylpurine + 2.5 mg / L of naphthalene acetic acid + 140 g / L of potato juice + 25 g / L of sucrose + pH 6.0; liquid culture conditions For, under the conditions of 18 degrees Celsius, rotating speed of 180 rpm, illuminance of 1800 lux, and light time of 4 hours / day, shaking culture for 15 days, the seeds germinated and formed protocorms, see figure 2 B, the fresh weight of 500 protocorms is 2.358 grams, and the dry weight is 0.246 grams.

[0028] Place the obtained protocorms in the differentiation medium and culture th...

Embodiment 3

[0032] Soak the Bletilla striata capsules in alcohol for 50 seconds, then soak them in an aqueous solution of sodium hypochlorite with an available chlorine concentration of 0.6% for 12 minutes, and then rinse them with sterile water for 3 to 4 times.

[0033] Cut the sterilized capsules, sow the Bletilla striata seeds in the liquid medium, and culture them with vibration; see the growth condition of the seeds after germination. figure 1 C; The composition of the liquid medium is: 1 / 4MS medium + 6-benzylpurine 0.8 mg / L + naphthalene acetic acid 2.8 mg / L + potato juice 150 g / L + sucrose 25 g / L + pH 5.8; liquid culture conditions For, under the conditions of 18 degrees Celsius, rotating speed of 230 rpm, illuminance of 2200 lux, and light time of 4 to 5 hours / day, shake culture for 20 days, the seeds germinate and form protocorms, see figure 2 C, the fresh weight of 500 protocorms is 2.246 grams, and the dry weight is 0.231 grams.

[0034] Place the obtained protocorms in the...

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Abstract

The invention aims at providing a method for efficiently and quickly producing a bletilla striata seedling. The method comprises (1) a liquid culture stage and (2) a solid medium culture stage, wherein (1) the liquid culture stage comprises the particular steps that a bletilla striata capsule is disinfected via sodium hypochlorite; the capsule is cut; a seed is sown in a liquid medium for culture; the seed is developed into a protocorm; (2) the solid medium culture stage comprises the particular steps that the protocorm is induced to be differentiated into a root-free seedling, and then transferred to a rooting culture medium for rooting culture, and a cultivation substrate transplanted into a greenhouse is domesticated before field transplanting. The method realizes efficient and quick production of the bletilla striata seedling; compared with whole-course solid culture, the method overcomes the defect that the seed germination rate is low when an early seed is cultured on a solid medium; the seed germination rate is increased significantly and reaches 99%; the fresh weight and the dry weight of the protocorm are increased greatly; and the whole production cycle is shortened significantly, and can be shortened to about 110 days.

Description

technical field [0001] The invention belongs to the technical field of rapid plant propagation, and in particular relates to a method for efficiently and rapidly producing bletilla striata seedlings. Background technique [0002] Bletilla striata ( Bletilla Striata (Thunb.) Reichb.f.) is a perennial herbaceous plant of the genus Bletilla striata. It is a national key protected wild plant. It not only has high ornamental value, but is also one of the commonly used traditional Chinese medicines. Bletilla striata is used as medicine with dried tubers, which has the effects of astringent and hemostasis, detumescence and granulation; it has good curative effect on hemoptysis, hematemesis, blood in the stool and trauma hemostasis, sore swelling, cracked hands and feet, etc. It is widely used in the treatment of pulmonary tuberculosis and bronchiectasis hemoptysis , gastric ulcer bleeding and other diseases, bletilla striata is mainly sold to hospitals, biopharmaceutical companies...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 孙力军张卫明姚正颖赵伯涛
Owner NANJING INST FOR THE COMPREHENSIVE UTILIZATION OF WILD PLANTS CHINA COOP
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