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Glutathione sensor, preparation method thereof and application thereof in capillary electrophoresis amperometric detection

A glutathione and sensor technology, applied in the field of sensors, can solve the problems of high detection limit and low sensitivity, and achieve the effects of fast response speed, high sensitivity and wide linear range

Inactive Publication Date: 2014-05-21
SHANDONG NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, these detection limits are high and the sensitivity is not high

Method used

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  • Glutathione sensor, preparation method thereof and application thereof in capillary electrophoresis amperometric detection
  • Glutathione sensor, preparation method thereof and application thereof in capillary electrophoresis amperometric detection
  • Glutathione sensor, preparation method thereof and application thereof in capillary electrophoresis amperometric detection

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Experimental program
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Effect test

Embodiment 1

[0024] Embodiment 1 Preparation of glutathione sensor

[0025] Before chemical modification, the surface of the carbon fiber electrode must be cleaned. The specific method is to carefully polish it on metallographic sandpaper to make the surface of the electrode smooth, and then ultrasonically in secondary water, ethanol, and secondary water for 5 minutes each. Let dry at room temperature. Before each test, the graphene oxide solution was ultrasonically dispersed in an ultrasonic cleaner for 30 minutes. In order to prepare graphene oxide-modified carbon fiber electrodes, we immersed the cross-section of the carbon fiber electrodes in the graphene oxide solution for 15 seconds. Avoid immersing the carbon fiber electrodes in the graphene oxide solution too deeply, so as not to expand the active surface of the electrode. Take out the electrode and let it dry at room temperature. Then put the dried graphene oxide modified electrode into 0.1mol / L phosphate buffer solution contain...

Embodiment 2

[0027]Before chemical modification, the surface of the carbon fiber electrode must be cleaned. The specific method is to carefully polish it on metallographic sandpaper to make the surface of the electrode smooth, and then ultrasonically in the secondary water, ethanol, and secondary water for 3 minutes. Let dry at room temperature. Before each test, the graphene oxide solution was ultrasonically dispersed in an ultrasonic cleaner for 30 minutes. In order to prepare graphene oxide-modified carbon fiber electrodes, we immersed the cross-section of the carbon fiber electrodes in the graphene oxide solution for 20 s. Avoid immersing the carbon fiber electrodes in the graphene oxide solution too deeply, so as not to expand the active surface of the electrode. Take out the electrode and let it dry at room temperature. Then put the dried graphene oxide modified electrode into 0.1mol / L phosphate buffer solution containing pH 7.0, and electrodeposit it at -1.1V for 200s. After takin...

Embodiment 3

[0029] Before chemical modification, the surface of the carbon fiber electrode must be cleaned. The specific method is to carefully polish it on metallographic sandpaper to make the surface of the electrode smooth, and then ultrasonically in the secondary water, ethanol, and secondary water for 3 minutes. Let dry at room temperature. Before each test, the graphene oxide solution was ultrasonically dispersed in an ultrasonic cleaner for 20 minutes. In order to prepare graphene oxide-modified carbon fiber electrodes, we immersed the cross-section of the carbon fiber electrodes in the graphene oxide solution for 18s. Avoid immersing the carbon fiber electrodes in the graphene oxide solution too deeply, so as not to expand the active surface of the electrode. Take out the electrode and let it dry at room temperature. Then put the dried graphene oxide modified electrode into 0.1mol / L phosphate buffer solution containing pH 7.0, and electrodeposit it at -0.8V for 300s. After takin...

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PUM

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Abstract

The invention relates to a glutathione sensor, a preparation method thereof and application thereof in capillary electrophoresis amperometric detection. The preparation method comprises the following steps: polishing the cross section of a carbon fiber micro-disk electrode to be smooth, carrying out ultrasonic cleaning on the polished carbon fiber micro-disk electrode, and immersing the cleaned cross section of the carbon fiber micro-disk electrode into a 0.3gm / mL-0.7mg / mL of graphene oxide solution for 10-20s, and then drying in the air; reducing the graphene oxide on the carbon fiber micro-disk electrode, wherein the electro-deposition potential is at constant potential of -1.2 to -0.7V, the electricity reduction time is 150-370s, and the obtained electrode is the glutathione sensor. The sensor electrode is wide in linear range, high in sensitivity and fast in response speed, electroactive materials possible in actual samples such as dopamine, epinephrine, cysteine and the like have no interference to the determination of the glutathione.

Description

technical field [0001] The present invention relates to a sensor, especially a glutathione sensor, its preparation method and its application in the determination of glutathione content in glutathione eye drops by capillary electrophoresis amperometry, which belongs to electroanalytical chemical detection technology field. Background technique [0002] Chemically modified electrodes are various modifications on conductors or semiconductor electrodes, so as to endow the electrodes with certain special properties, which can have more sensitive response signals to certain substances, and overcome the overpotential that unmodified electrodes tend to appear in the measurement It has been widely used in the field of analytical chemistry, especially in the preparation of biosensors. [0003] Commonly used chemically modified electrodes include chemically modified carbon paste microelectrodes, metal and nonmetal nanoparticle modified microelectrodes, surface molecular film modified...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/447
Inventor 王晓蕾王军刘冬菊赵满
Owner SHANDONG NORMAL UNIV
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