Halomonas huanghegensis BJGMM-B45 and application thereof
A technology of BJGMM-B45 and Halomonas, applied in the field of microorganisms, can solve problems that restrict agricultural development and affect sustainable development
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Embodiment 1
[0036] The isolation culture method of embodiment 1 Halomonas yellow river BJGMM-B45
[0037] Soil samples were collected from undisturbed saline-alkali areas in the Yellow River Delta Nature Reserve. Weigh about 1g of soil sample, add it to 9ml of 0.9% normal saline, and shake the bacteria at 30°C for 24h. Then perform gradient dilution, spread on beef extract peptone medium (0.5g beef extract, 1.0g peptone, 0.5g NaCl, 1.5-2.0g agar, add water to 100ml, pH7.2), separate and cultivate soil sample bacteria. On beef extract peptone medium with a NaCl concentration of 150g / l, flip the plate and place it in an incubator at 30°C for 2 days, then isolate and cultivate Halomonas huanghegensis (Halomonas huanghegensis) BJGMM-B45 of the present invention, its preservation number It is CGMCC NO.7022. The colonies are white, round, with neat edges, raised and smooth surface, and 0.5-1cm in diameter.
Embodiment 2
[0038] The salt tolerance test of embodiment 2 Halomonas yellow river BJGMM-B45
[0039] 1.1 Test method
[0040] Using LB liquid medium with different NaCl concentrations (0, 10, 20, 50, 80, 100, 110, 130, 150, 180 and 200g / l) to identify the optimal salinity tolerance of Halomonas yellow Bacillus (E.coli K12, CGMCC1.365) was used as a control. Prepare the seed solution, insert it into LB liquid medium with 5% inoculum amount, cultivate it at 30°C, 200r / min for 12 hours, measure the OD value at 600nm, and compare their growth conditions.
[0041] 1.2 Results
[0042] The OD value at 600nm of the strain BJGMM-B45 was 6 times higher than that of the control strain in the salt environment of 80, 100, 110, and 130g / L NaCl respectively (Table 2 and figure 2 ).
[0043] Table 2. The growth of Halomonas yellowheus BJGMM-B45 under different salt concentrations
[0044]
[0045] Note: ND means not determined.
Embodiment 3
[0046] Example 3 Antibiotic tolerance test of Halomonas yellowheus BJGMM-B45
[0047] 1.1 Test method
[0048] The LB liquid medium of different antibiotics was used to test the antibiotic resistance of Halomonas yellowheus: the working concentrations of chloramphenicol, streptomycin, ampicillin, rifampicin and tetracycline hydrochloride were: 25μg / ml, 50μg / ml , 50 μg / ml, 150 μg / ml and 50 μg / ml.
[0049] 1.2 Results
[0050] Halomonas yellow river BJGMM-B45 has antibiotic resistance, shows good resistance to chloramphenicol (25 μ g / ml) and streptomycin (50 μ g / ml) ( image 3 ).
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