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Descendant determining method for the development of yellow catfishes, snakehead super male fishes and all male fishes

A progeny determination and yellow catfish technology, which is applied to the field of progeny determination developed by yellow catfish, snakehead super male and full male fish, can solve the problems of deviation of the sex ratio of the sampling group from the theoretical ratio, uncertainty of the determination result, etc. Simple operation, low breeding risk, and the effect of improving the efficiency of test crosses

Active Publication Date: 2014-05-21
FOSHAN NANHAI BAIRONG AQUATIC SEED +1
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Problems solved by technology

However, in the actual breeding operation process, due to the occurrence of breeding conditions, sampling quantity, and the occurrence of killings accompanying the fry breeding process, the sex ratio of the sampling population deviates seriously from the theoretical ratio, which makes it necessary to carry out parental genetic analysis strictly based on the sex ratio of offspring. There are great uncertainties in the judgment results of the method of type distinction

Method used

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Embodiment Construction

[0014] In order for those skilled in the art to better understand the technical solutions provided by the present invention, the following will be described in conjunction with specific embodiments.

[0015] The progeny determination method for the development of yellow catfish and snakehead super male fish and all male fish provided by the embodiments of the present invention comprises the following steps:

[0016] 1. Construction of an all-female population

[0017] (1) Induction of candidate pseudo-male populations: In the breeding season, male and female parents of normal-sexed yellow catfish or black snakehead are taken, artificially induced, and the dose of induced labor is the first injection of LHRH-A2 (progesterone-releasing hormone A2) for the female parent of yellow catfish. 3ug / Kg, inject the second injection of LHRH-A210ug / Kg+DOM (diomone) 5mg / Kg+HCG1300IU / Kg 12 hours later, the male fish will be injected with oxytocic agent at the second injection of female fish,...

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Abstract

The invention discloses a descendant determining method for the development of yellow catfishes, snakehead super male fishes and all male fishes. The method comprises the following steps: (1) controlling the sex by the hormone of the yellow catfishes and the snakehead super male fishes; (2) testcrossing and screening pseudo males; (3) obtaining all female groups and pseudo female groups; (4) testcrossing by the pseudo males and screening the pseudo females; (5) testcrossing by the all female groups and screening YY males and YY females to form super male lines. Compared with the prior art, the descendant determining method for the development of the yellow catfishes, the snakehead super male fishes and the all male fishes has the advantages of easiness in operation, high efficiency, low risk in breeding and high testcross efficiency, overcomes the problem of the uncertainty for traditionally developing the all male offspring seeds of XX-XY genotype farmed animals, and reduces the counting difficult.

Description

technical field [0001] The invention relates to the field of genetic breeding of aquatic animals, in particular to a method for determining the descendants of yellow catfish, snakehead super-male and all-male development. Background technique [0002] In aquaculture, there are differences in economic traits between male and female individuals of many cultured species, such as growth rate, individual size and size of edible (medicinal) parts. Therefore, the production of single-sex seedlings for breeding can significantly improve economic benefits by controlling sex. For example, yellow catfish or black snakehead, male fish grow faster than female fish, and the final individual is larger. Therefore, selecting all-male or male-majority fry during the breeding process can significantly increase the breeding efficiency. [0003] At present, the conventional methods for cultivating parthenogenetic seedlings mainly include hormone feeding method, distant hybridization, artificial...

Claims

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Application Information

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IPC IPC(8): A01K61/00
CPCY02A40/81
Inventor 韩林强吴建开江谢武古勇明胡重江许治冲李朝兵
Owner FOSHAN NANHAI BAIRONG AQUATIC SEED
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