Descendant determining method for the development of yellow catfishes, snakehead super male fishes and all male fishes
A progeny determination and yellow catfish technology, which is applied to the field of progeny determination developed by yellow catfish, snakehead super male and full male fish, can solve the problems of deviation of the sex ratio of the sampling group from the theoretical ratio, uncertainty of the determination result, etc. Simple operation, low breeding risk, and the effect of improving the efficiency of test crosses
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[0014] In order for those skilled in the art to better understand the technical solutions provided by the present invention, the following will be described in conjunction with specific embodiments.
[0015] The progeny determination method for the development of yellow catfish and snakehead super male fish and all male fish provided by the embodiments of the present invention comprises the following steps:
[0016] 1. Construction of an all-female population
[0017] (1) Induction of candidate pseudo-male populations: In the breeding season, male and female parents of normal-sexed yellow catfish or black snakehead are taken, artificially induced, and the dose of induced labor is the first injection of LHRH-A2 (progesterone-releasing hormone A2) for the female parent of yellow catfish. 3ug / Kg, inject the second injection of LHRH-A210ug / Kg+DOM (diomone) 5mg / Kg+HCG1300IU / Kg 12 hours later, the male fish will be injected with oxytocic agent at the second injection of female fish,...
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