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Application of fullerene as conditioning agent for conditioning growth or metabolism of microorganism

A technology of fullerenes and microorganisms, applied in the field of microorganisms, can solve the problems that there are no reported beneficial regulation effects of fullerenes, and the structure and function of the fullerene community of carbon nanomaterials are not disclosed, so as to improve the physical and chemical properties and flavor, and improve the function , the effect of promoting efficiency

Inactive Publication Date: 2014-05-14
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, none of these studies reported beneficial modulatory effects of fullerenes on mixed or pure microbial fermentation
That is, there is no disclosure of the carbon nanomaterial fullerene in regulating the community structure and function of the mixed microbial community, as well as the new use and method of regulating the growth and metabolic activities of microorganisms

Method used

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  • Application of fullerene as conditioning agent for conditioning growth or metabolism of microorganism
  • Application of fullerene as conditioning agent for conditioning growth or metabolism of microorganism
  • Application of fullerene as conditioning agent for conditioning growth or metabolism of microorganism

Examples

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Embodiment 1

[0040] This embodiment relates to the preparation of a carbon nanomaterial fullerene aqueous solution.

[0041] Reagents and instruments: Fullerene powder with a purity of 99.9% (Mer Corporation, USA), chromatographically pure toluene (Merck KGaA, Germany), ultrapure water system (Millipore water purification unit, Milli Q, USA), rotary evaporator (Switzerland) Buchi, Buchi Rotavapor).

[0042] Method: Weigh 40mg of fullerene (C 60 ) The powder is dissolved in 100 mL of toluene, and the container is placed on a shaker to shake and dissolve for 2 hours. After the fullerene is completely dissolved in toluene, add 400 mL of deionized water. The density of toluene is less than that of water, and the color of toluene floating on the upper layer is purple red. Seal the bottle tightly with parafilm to prevent toluene volatilization. The container was placed on an ultrasonic cleaner and sonicated for 96 hours, and the fullerene in toluene was transferred into the water phase by the holes...

Embodiment 2

[0045] This embodiment relates to the inhibitory effect of carbon nano-material fullerene on the growth of E. coli.

[0046] Reagents and equipment: Minimal Davis (MD) medium (1L): 7.0g K2HPO4, 3.0g KH2PO4, 1.0g (NH4)2SO4, 0.5g sodium citrate trihydrate, 0.1g MgSO4·7H2O, 0.02g arginine, 0.02g tryptophan, 2g glucose. 1mg / L nC 60 , 5mg / L nC 60 , Automatic microbial growth curve tester (Finland Bioscreen C, MBR), supporting multi-well plate.

[0047] Method: Inoculate logarithmic phase E.coli into 1000μL MD medium containing 1mg / L and 5mg / L according to 1% inoculation amount, and the system is marked for easy spotting. After vortexing to mix well, transfer the microsystem inoculated with each sample to a microwell plate with 300μL for each sample inlet and three replicates for each sample. After the self-test of the Bioscreen automatic growth curve analyzer is completed, place the sampled multiwell plate in the multiwell plate rack in the instrument. Open the program setting softwa...

Embodiment 3

[0050] This embodiment relates to the growth-promoting effect of fullerene on Bacillus cereus.

[0051] Reagents and equipment: Minimal Davis medium (the formula is the same as in Example 2), 1mg / L nC 60 , 5mg / L nC 60 Health, automatic microbial growth curve analyzer (Finland Bioscreen C, MBR), supporting multi-well plate.

[0052] Method: Inoculate logarithmic phase Bacillus cereus into 1000μL MD medium containing 1mg / L and 5mg / L according to 1% inoculation amount, and the system is marked for easy spotting. After vortexing to mix well, transfer the microsystem inoculated with each sample to a microwell plate with 300μL for each sample inlet and three replicates for each sample. After the self-test of the Bioscreen automatic growth curve analyzer is completed, place the sampled multiwell plate in the multiwell plate rack in the instrument. Open the program setting software, set the incubation temperature to 37°C, and with medium-intensity continuous shaking, the incubation time i...

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Abstract

The invention discloses an application of fullerene as a conditioning agent for conditioning growth or metabolism of microorganisms. The study on biological effects of fullerene confirms that fullerene has the functions of conditioning the growth and metabolism of microorganisms, a novel application of a fullerene material is developed, and the application of fullerene in microorganisms and relevant fields is prompted. The invention shows that fullerene has the following specific applications: 1, inhibiting growth of pathogenic bacteria or harmful bacteria; 2, prompting growth and metabolic activity of functional bacteria, and improving expression of intracellular total protein amount; 3, conditioning the community structures of microorganisms so as to improve the functions of communities; 4, improving soil fertility; 5, improving the physicochemical property and favor of a fermented product; and 6, improving the efficiency in environment remediation such as sewage treatment.

Description

Technical field [0001] The invention belongs to the technical field of microorganisms, and specifically relates to the use of fullerenes as a regulator for regulating the growth or metabolism of microorganisms. Background technique [0002] Nanomaterials refer to the general term for natural or man-made materials in three-dimensional space that have one dimension and above with nano-scale standard dimensions, or are composed of nano-scale particulate matter as units. Nanomaterials can be divided into metallic nanomaterials and non-metallic nanomaterials according to their composition. Among them, carbon nanomaterials are a kind of non-metallic nanomaterials that are widely studied and applied. As a typical carbon nanomaterial, fullerene has a wide range of applications in practice. [0003] Fullerene (Fullerene) is a hollow molecule composed entirely of carbon, with a spherical, ellipsoidal, cylindrical or tubular shape. C has been found 44 , C 50 , C 60 , C 76 , C 80 , C 84 , C...

Claims

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Application Information

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IPC IPC(8): C12N1/38
Inventor 张晓君黄飞葛玲赵立平张波何义亮
Owner SHANGHAI JIAO TONG UNIV
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